Towards pain-free diagnosis of skin diseases through multiplexed microneedles: biomarker extraction and detection using a highly sensitive blotting method

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• 作者：Keng Wooi Ng ; Wing Man Lau ; Adrian C. Williams
• 关键词：Immunodiagnostic microneedles ; Microprojection arrays ; Biomarkers ; Immobilised antibody ; Densitometry ; Skin diagnosis
• 刊名：Drug Delivery and Translational Research
• 出版年：2015
• 出版时间：August 2015
• 年：2015
• 卷：5
• 期：4
• 页码：387-396
• 全文大小：4,217 KB
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• 作者单位：Keng Wooi Ng (1)
  Wing Man Lau (2)
  Adrian C. Williams (2)
  
  1. School of Pharmacy and Biomolecular Sciences, University of Brighton, Huxley Building, Lewes Road, Brighton, BN2 4GJ, UK
  2. Reading School of Pharmacy, University of Reading, Whiteknights, P.O. Box 226, Reading, RG6 6AP, UK
  
• 刊物主题：Pharmaceutical Sciences/Technology;
• 出版者：Springer US
• ISSN：2190-3948

文摘

Immunodiagnostic microneedles provide a novel way to extract protein biomarkers from the skin in a minimally invasive manner for analysis in vitro. The technology could overcome challenges in biomarker analysis specifically in solid tissue, which currently often involves invasive biopsies. This study describes the development of a multiplex immunodiagnostic device incorporating mechanisms to detect multiple antigens simultaneously, as well as internal assay controls for result validation. A novel detection method is also proposed. It enables signal detection specifically at microneedle tips and therefore may aid the construction of depth profiles of skin biomarkers. The detection method can be coupled with computerised densitometry for signal quantitation. The antigen specificity, sensitivity and functional stability of the device were assessed against a number of model biomarkers. Detection and analysis of endogenous antigens (interleukins 1α and 6) from the skin using the device was demonstrated. The results were verified using conventional enzyme-linked immunosorbent assays. The detection limit of the microneedle device, at ?0?pg/mL, was at least comparable to conventional plate-based solid-phase enzyme immunoassays.