Light induced transmembrane proton gradient in artificial lipid vesicles reconstituted with photosynthetic reaction centers

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• 作者：Francesco Milano (1)
  Massimo Trotta (1) m.trotta@ba.ipcf.cnr.it
  Márta Dorogi (2)
  Béla Fischer (2)
  Livia Giotta (3)
  Angela Agostiano (4)
  Péter Maróti (2)
  László Kálmán (5)
  László Nagy (2)
• 关键词：Reaction centers – Proton motive force – Ionophores – Pyranine
• 刊名：Journal of Bioenergetics and Biomembranes
• 出版年：2012
• 出版时间：June 2012
• 年：2012
• 卷：44
• 期：3
• 页码：373-384
• 全文大小：381.2 KB
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• 作者单位：1. Institute for Physical and Chemical Processes (IPCF), Bari division, Italian National Research Council (CNR), Via Orabona 4, 70126 Bari, Italy2. Institute of Medical Physics and Informatics, University of Szeged, Rerrich B. tér 1, 6720 Szeged, Hungary3. Department of Material Sciences, University of Salento, Strada per Monteroni, 73100 Lecce, Italy4. Department of Chemistry, University of Bari, Via Orabona 4, 70126 Bari, Italy5. Department of Physics, Concordia University, Montreal, QC, Canada
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Bioorganic Chemistry
  Biochemistry
  Animal Anatomy, Morphology and Histology
  Animal Biochemistry
  Organic Chemistry
  
• 出版者：Springer New York
• ISSN：1573-6881

文摘

Photosynthetic reaction center (RC) is the minimal nanoscopic photoconverter in the photosynthetic membrane that catalyzes the conversion of solar light to energy readily usable for the metabolism of the living organisms. After electronic excitation the energy of light is converted into chemical potential by the generation of a charge separated state accompanied by intraprotein and ultimately transmembrane proton movements. We designed a system which fulfills the minimum structural and functional requirements to investigate the physico/chemical conditions of the processes: RCs were reconstituted in closed lipid vesicles made of selected lipids entrapping a pH sensitive indicator, and electron donors (cytochrome c₂ and K₄[Fe(CN)₆]) and acceptors (decylubiquinone) were added to sustain the photocycle. Thanks to the low proton permeability of our preparations, we could show the formation of a transmembrane proton gradient under illumination and low buffering conditions directly by measuring proton-related signals simultaneously inside and outside the vesicles. The effect of selected ionophores such as gramicidin, nigericin and valinomycin was used to gain more information on the transmembrane proton gradient driven by the RC photochemistry.