文摘
The objective of the present study was to investigate the transport and removal of Escherichia coli, Bacillus subtilis, Staphylococcus aureus, bacteriophage MS2, and bacteriophage Phix174 in the soils and pyrophyllite-amended soils. Laboratory columns experiments were performed under saturated flow conditions. Our results showed that bacteriophages passed through the soils more easily than bacteria under the given experimental conditions (pulse injection = 15 min, flow rate = 0.5 mL/min, column length = 20 cm, inner diameter = 2.5 cm, pH = 7.6, electrical conductivity (EC) = 150.1 μS/cm); the log removals of bacteria were in the range of 0.44 to 1.72, whereas the log removals of bacteriophages were between 0.01 and 0.13. Our results also demonstrated that the transport of bacteria and bacteriophages in the soil columns could be reduced considerably in the presence of pyrophyllite. Under the same column experimental conditions above, the log removals for MS2 and Phix174 in 50% soil + 50% pyrophyllite were 2.64 and 3.05, respectively, whereas the log removals in 100% pyrophyllite were 5.70 for MS2 and 5.10 for Phix174; those values were far greater than the log removals in 100% soil (MS2 = 0.063, Phix174 = 0.128). Additional column experiments (step injection, flow rate = 0.3 mL/min, column length = 30 cm, inner diameter = 2.5 cm, solution pH = 8.4, EC = 39.8 mS/cm) showed that the log removals for B. subtilis (1.72) and Phix174 (1.48) in the pyrophyllite were greater than those in the soil (B. subtilis = 1.41; Phix174 = 0.39). This study demonstrated that the pyrophyllite amendment method could be used for protecting groundwater from microbial contamination by animal carcass burial soils.