Nitric oxide heme interactions in nitrophorin from Cimex lectularius

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• 作者：R. Christmann ; H. Auerbach ; R. E. Berry ; F. A. Walker…
• 关键词：Nitric oxide ; Mössbauer spectroscopy ; Density functional theory ; Nitrophorin ; Heme
• 刊名：Hyperfine Interactions
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：237
• 期：1
• 全文大小：1,216 KB
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• 作者单位：R. Christmann (1)
  H. Auerbach (1)
  R. E. Berry (2)
  F. A. Walker (2)
  V. Schünemann (1)
  
  1. Department of Physics, University of Kaiserslautern, Erwin-Schrödinger-Str. 46, 67663, Kaiserslautern, Germany
  2. Department of Chemistry and Biochemistry, The University of Arizona, Tucson, AZ, 85721-0041, USA
  
• 刊物类别：Physics and Astronomy
• 刊物主题：Physics
  Nuclear Physics, Heavy Ions and Hadrons
  Atoms, Molecules, Clusters and Plasmas
  Condensed Matter
  Surfaces and Interfaces and Thin Films
  
• 出版者：Springer Netherlands
• ISSN：1572-9540

文摘

The nitrophorin from the bedbug Cimex lectularius (cNP) is a nitric oxide (NO) carrying protein. Like the nitrophorins (rNPs) from the kissing bug Rhodnius prolixus, cNP forms a stable heme Fe(III)-NO complex, where the NO can be stored reversibly for a long period of time. In both cases, the NPs are found in the salivary glands of blood-sucking bugs. The insects use the nitrophorins to transport the NO to the victim’s tissues, resulting in vasodilation and reduced blood coagulation. However, the structure of cNP is significantly different to those of the rNPs from Rhodnius prolixus. Furthermore, the cNP can bind a second NO molecule to the proximal heme cysteine when present at higher concentrations. High field Mössbauer spectroscopy on 57Fe enriched cNP complexed with NO shows reduction of the heme iron and formation of a ferrous nitric oxide (Fe(II)-NO) complex. Density functional theory calculations reproduce the experimental Mössbauer parameters and confirm this observation.