Zinc Distribution and Expression Pattern of ZnT3 in Mouse Brain

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• 作者：Hui Shen (1)
  Fudi Wang (1)
  Yuanxun Zhang (2)
  Jiajun Xu (3)
  Jiangang Long (1)
  Haigong Qin (1)
  Fang Liu (3)
  Junsheng Guo (1)
  
• 关键词：Zinc ; Zinc transporter 3(ZnT3) ; Synchrotron radiation x ; ray fluorescence (SRXRF) ; Reverse transcription polymerase chain reaction (RT ; PCR) ; In situ hybridization (ISH)
• 刊名：Biological Trace Element Research
• 出版年：2007
• 出版时间：November 2007
• 年：2007
• 卷：119
• 期：2
• 页码：166-174
• 全文大小：409KB
• 参考文献：1. Frederickson CJ, Klitenick MA, Manton WI, Kirkpatrick JB (1983) Cytoarchitectonic distribution of zinc in the hippocampus of man and the rat. Brain Res 273:335-39 CrossRef
  2. Wallwork JC, Sandstead HH (1993) Zinc and brain function. Prog Clin Biol Res 380:65-0
  3. Wang FD, Zhao FJ, Guo JS, Jing NH, Ma XF, Mei ZT (1998) Effects of zinc on zinc deficiency during pregnancy and lactation of maternal mice on learning ability and hippocampal long-term potentiation of offsprings. Chin J Neurosci 14:20-3
  4. Wang FD, Bian W, Kong LW, Zhao FJ, Guo JS, Jing NH (2001) Maternal zinc deficiency impairs brain nestin expression in prenatal and postnatal mice. Cell Res 11:135-41 CrossRef
  5. Chimienti F, Aouffen M, Favier A, Seve M (2003) Zinc homeostasis-regulating proteins: new drug targets for triggering cell fate. Curr Drug Targets 4:323-38 CrossRef
  6. Kagi JH, Valee BL (1960) Metallothionein: a cadmium- and zinc-containing protein from equine renal cortex. J Biol Chem 235:3460-465
  7. Cherian MG, Howell SB, Imura N, Klaassen CD, Koropatnick J, Lazo JS, Waalkes MP (1994) Role of metallothionein in carcinogenesis. Toxicol Appl Pharmacol 126:1- CrossRef
  8. Eide DJ (1997) Molecular biology of iron and zinc uptake in eukaryotes. Curr Opin Cell Biol 9:573-77 CrossRef
  9. Eide DJ (2004) The SLC39 family of metal ion transporters. Pflugers Arch 447:796-00 CrossRef
  10. Kambe T, Yamaguchi-Iwai Y, Sasaki R, Nagao M (2004) Overview of mammalian zinc transporters. Cell Mol Life Sci 61:49-8 CrossRef
  11. Seve M, Chimienti F, Devergnas S, Favier A (2004) In silico identification and expression of SLC30 family genes: an expressed sequence tag data mining strategy for the characterization of zinc transporters-tissue expression. BMC Genomics 5:32 CrossRef
  12. Palmiter RD, Cole TB, Quaife CJ, Findley SD (1996) ZnT-3, a putative transporter of zinc into synaptic vesicles. Proc Natl Acad Sci U S A 93:14934-4939 CrossRef
  13. Zhang Y, Cheng F, Li D, Wang Y, Zhang G, Liao W, Tang T, Huang Y, He W (2005) Investigation of elemental content distribution in femoral head slice with osteoporosis by SRXRF microprobe. Biol Trace Elem Res 103:177-85 CrossRef
  14. Dagerlind A, Friberg K, Bean AJ, Hokfelt T (1992) Sensitive mRNA detection using unfixed tissue: combined radioactive and non-radioactive in situ hybridization histochemistry. Histochemistry 98:39-9 CrossRef
  15. Shi JY, Chen YX, Huang YY, He W (2004) SRXRF microprobe as a technique for studying elements distribution in / Elsholtzia splendends. Micron 35:557-64 CrossRef
  16. Frederickson CJ (1989) Neurobiology of zinc and zinc-containing neurons. Int Rev Neurobiol 31:145-38 CrossRef
  17. Takeda A (2000) Movement of zinc and its functional significance in the brain. Brain Res Brain Res Rev 34:137-48 CrossRef
  18. Thompson RB, Peterson D, Mahoney W, Cramer M, Maliwal BP, Suh SW, Frederickson C, Fierke C, Herman P (2002) Fluorescent zinc indicators for neurobiology. J Neurosci Methods 118:63-5 CrossRef
  19. Gaither LA, Eide DJ (2001) The human ZIP1 transporter mediates zinc uptake in human K562 erythroleukemia cells. J Biol Chem 276:22258-2264 CrossRef
  20. Taylor KM, Nicholson RI (2003) The LZT proteins: the LIV-1 subfamily of zinc transporters. Biochim Biophys Acta 1611:16-0 CrossRef
  21. Jo SM, Danscher G, Daa-Schroder H, Won MH, Cole TB (2000) Zinc-enriched (ZEN) terminals in mouse spinal cord: immunohistochemistry and autometallography. Brain Res 870:163-69 CrossRef
  22. Wang ZY, Danscher G, Dahlstrom A, Li JY (2003) Zinc transporter 3 and zinc ions in the rodent superior cervical ganglion neurons. Neuroscience 120:605-16 CrossRef
  23. Danscher G, Wang Z, Kim YK, Kim SJ, Sun Y, Jo SM (2003) Immunocytochemical localization of zinc transporter 3 in the ependyma of the mouse spinal cord. Neurosci Lett 342:81-4 CrossRef
  24. Suh SW, Jo SM, Vajda Z, Danscher G (2005) Adrenalectomy-induced ZnT3 downregulation in mouse hippocampus is followed by vesicular zinc depletion. Neurosci Lett 377:164-69 CrossRef
  
• 作者单位：Hui Shen (1)
  Fudi Wang (1)
  Yuanxun Zhang (2)
  Jiajun Xu (3)
  Jiangang Long (1)
  Haigong Qin (1)
  Fang Liu (3)
  Junsheng Guo (1)
  
  1. Department of Military Hygiene, Second Military Medical University, Shanghai, 200433, China
  2. Institute of Applied Physics, Chinese Academy of Sciences, Shanghai, 201800, China
  3. Department of Anatomy, Second Military Medical University, Shanghai, 200433, China
  

文摘

To explore the relationship between the zinc distribution and zinc transporter 3 (ZnT3) mRNA expression in the mouse brain, zinc contents and its distribution were determined by synchrotron radiation x-ray fluorescence (SRXRF), and ZnT3 mRNA expression was examined by reverse-transcription polymerase chain reaction and in situ hybridization. The results showed that the zinc contents were not distributed evenly in various brain tissues. The zinc contents in cerebral cortex and hippocampus were nearly 5-0 times higher than that in other neural locations. Correspondingly, ZnT3 mRNA expression was observed in high abundance in the cerebral cortex, hippocampus, and testis, but was not detected in other organs and tissues. In the nervous system, ZnT3 mRNA was detected mainly in hippocampus, cerebral cortex, and spinal ganglion. The present results show the coincident distribution of zinc and ZnT3 mRNA in mouse brain. The high zinc contents might be determined by the high expression of ZnT3. More meaningfully, the results showed the feasibility of applying of SRXRF in examining the distribution of minerals in different organs and tissues. In addition, it was observed for the first time that ZnT3 mRNA was expressed in the facial nucleus. The function of ZnT3 in facial nucleus awaits further study.