Evaluation of arsenic trioxide genotoxicity in wheat seedlings using oxidative system and RAPD assays
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- 作者：Ozkan Aksakal ; Nevzat Esim
- 关键词：As2O3 ; Catalase ; MDA ; Peroxidase ; RAPD ; Superoxide dismutase
- 刊名：Environmental Science and Pollution Research
- 出版年：2015
- 出版时间：May 2015
- 年：2015
- 卷：22
- 期：9
- 页码：7120-7128
- 全文大小：552 KB
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Patra - 作者单位：Ozkan Aksakal (1)
Nevzat Esim (2)
1. Department of Biology, Science Faculty, Atatürk University, 25240, Erzurum, Turkey
2. Vocational Training School, Bing?l University, Bing?l, Turkey - 刊物类别：Earth and Environmental Science
- 刊物主题：Environment
Environment
Atmospheric Protection, Air Quality Control and Air Pollution
Waste Water Technology, Water Pollution Control, Water Management and Aquatic Pollution
Industrial Pollution Prevention - 出版者：Springer Berlin / Heidelberg
- ISSN：1614-7499
文摘
Arsenic is a metalloid that is toxic to living organisms. It is known that high concentration of arsenic causes toxic damage to cells and tissues of plants. While the toxic effect of arsenic is known, limited efforts have been made to study its genotoxic effect on the crops. In the present study, effects of arsenic trioxide (As2O3) on seed germination, root length, reactive oxygen species (ROS), lipid peroxidation (malondialdehyde (MDA)), and activities of antioxidant enzymes, as well as DNA in wheat seedlings were investigated. Seedlings were exposed to different (10 to 40?mg/L) As2O3 concentrations for 7?days. Seed germination and root elongation decreased with increase of As2O3 concentration. The values of hydrogen peroxide (H2O2), superoxide anion (O2 ·?/sup>), and MDA contents significantly increased by As2O3 concentrations. The highest values for H2O2, O2 ·?/sup>, and MDA were obtained in 40?mg/L treated wheat seedling. A significant increase of peroxidase (POX) and catalase (CAT) activity in seedlings were observed with increased concentration of As2O3, then decreased when reaching a value of 40?mg/L, whereas the activities of superoxide dismutase (SOD) were gradually enhanced with increasing As2O3 concentration. Alterations of DNA in wheat seedlings were detected using randomly amplified polymorphic DNA (RAPD) technique. The changes occurring in RAPD profiles of seedlings following As2O3 treatment included loss of normal bands and appearance of new bands in comparison to that of control seedlings. The results of our study showed that As2O3 induced DNA damage in a dose-dependent meaner, and the root cells of wheat studied showed a defense against As2O3-induced oxidative stress by enhancing their antioxidant activities.