Autophagy protects meniscal cells from glucocorticoids-induced apoptosis via inositol trisphosphate receptor signaling

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• 作者：Chao Shen ; Wen Gu ; Gui-Quan Cai ; Jian-Ping Peng ; Xiao-Dong Chen
• 关键词：Autophagy ; Meniscus ; Inositol trisphosphate receptor ; Dexamethasone ; Arthritis
• 刊名：Apoptosis
• 出版年：2015
• 出版时间：September 2015
• 年：2015
• 卷：20
• 期：9
• 页码：1176-1186
• 全文大小：5,063 KB
• 参考文献：1.Salata MJ, Gibbs AE, Sekiya JK (2010) A systematic review of clinical outcomes in patients undergoing meniscectomy. Am J Sports Med 38:1907鈥?916PubMed View Article
  2.Englund M, Roemer FW, Hayashi D, Crema MD, Guermazi A (2012) Meniscus pathology, osteoarthritis and the treatment controversy. Nat Rev Rheumatol 8:412鈥?19PubMed View Article
  3.MacMullan PA, McCarthy GM (2010) The meniscus, calcification and osteoarthritis: a pathologic team. Arthritis Res Ther 12:116PubMed Central PubMed View Article
  4.Cyriax J, Troisier O (1953) Hydrocortone and soft-tissue lesions. Br Med J 2:966鈥?68PubMed Central PubMed View Article
  5.Murrell GA, Doland MM, Jang D, Szabo C, Warren RF, Hannafin JA (1996) Nitric oxide: an important articular free radical. J Bone Joint Surg Am 78:265鈥?74PubMed
  6.Dragoo JL, Danial CM, Braun HJ, Pouliot MA, Kim HJ (2012) The chondrotoxicity of single-dose corticosteroids. Knee Surg Sports Traumatol Arthrosc 20:1809鈥?814PubMed View Article
  7.Braun HJ, Wilcox-Fogel N, Kim HJ, Pouliot MA, Harris AH, Dragoo JL (2012) The effect of local anesthetic and corticosteroid combinations on chondrocyte viability. Knee Surgery Sports Traumatol Arthrosc 20:1689鈥?695View Article
  8.Mistiaen WP, Somers P, Knaapen MW, Kockx MM (2006) Autophagy as mechanism for cell death in degenerative aortic valve disease. Autophagy 2:221鈥?23PubMed View Article
  9.Bredesen DE, Rao RV, Mehlen P (2006) Cell death in the nervous system. Nature 443:796鈥?02PubMed Central PubMed View Article
  10.Mizushima N, Komatsu M (2011) Autophagy: renovation of cells and tissues. Cell 147:728鈥?41PubMed View Article
  11.Shen C, Yan J, Jiang LS, Dai LY (2011) Autophagy in rat annulus fibrosus cells: evidence and possible implications. Arthritis Res Ther 13:R132PubMed Central PubMed View Article
  12.Carames B, Hasegawa A, Taniguchi N, Miyaki S, Blanco FJ, Lotz M (2012) Autophagy activation by rapamycin reduces severity of experimental osteoarthritis. Ann Rheum Dis 71:575鈥?81PubMed Central PubMed View Article
  13.Carames B, Taniguchi N, Otsuki S, Blanco FJ, Lotz M (2010) Autophagy is a protective mechanism in normal cartilage, and its aging-related loss is linked with cell death and osteoarthritis. Arthritis Rheum 62:791鈥?01PubMed Central PubMed View Article
  14.Shen C, Yan J, Erkocak OF, Zheng XF, Chen XD (2014) Nitric oxide inhibits autophagy via suppression of JNK in meniscal cells. Rheumatology 53:1022鈥?033PubMed View Article
  15.Yu L, Wan F, Dutta S et al (2006) Autophagic programmed cell death by selective catalase degradation. Proc Natl Acad Sci USA 103:4952鈥?957PubMed Central PubMed View Article
  16.White C, Li C, Yang J et al (2005) The endoplasmic reticulum gateway to apoptosis by Bcl-X(L) modulation of the InsP3R. Nat Cell Biol 7:1021鈥?028PubMed Central PubMed View Article
  17.Szlufcik K, Bultynck G, Callewaert G, Missiaen L, Parys JB, De Smedt H (2006) The suppressor domain of inositol 1,4,5-trisphosphate receptor plays an essential role in the protection against apoptosis. Cell Calcium 39:325鈥?36PubMed View Article
  18.Camandola S, Cutler RG, Gary DS, Milhavet O, Mattson MP (2005) Suppression of calcium release from inositol 1,4,5-trisphosphate-sensitive stores mediates the anti-apoptotic function of nuclear factor-kappaB. J Biol Chem 280:22287鈥?2296PubMed View Article
  19.Song YW, Zhang T, Wang WB (2012) Gluococorticoid could influence extracellular matrix synthesis through Sox9 via p38 MAPK pathway. Rheumatol Int 32:3669鈥?673PubMed View Article
  20.Decuypere J-P, Welkenhuyzen K, Luyten T et al (2011) IP3 receptor-mediated Ca2+ signaling and autophagy induction are interrelated. Autophagy 7:1472鈥?489PubMed Central PubMed View Article
  21.Harr MW, McColl KS, Zhong F, Molitoris JK, Distelhorst CW (2010) Glucocorticoids downregulate Fyn and inhibit IP(3)-mediated calcium signaling to promote autophagy in T lymphocytes. Autophagy 6:912鈥?21PubMed Central PubMed View Article
  22.Vicencio JM, Ortiz C, Criollo A et al (2009) The inositol 1,4,5-trisphosphate receptor regulates autophagy through its interaction with Beclin 1. Cell Death Differ 16:1006鈥?017PubMed View Article
  23.Criollo A, Maiuri MC, Tasdemir E et al (2007) Regulation of autophagy by the inositol trisphosphate receptor. Cell Death Differ 14:1029鈥?039PubMed
  24.Biederbick A, Kern HF, Elsasser HP (1995) Monodansylcadaverine (MDC) is a specific in vivo marker for autophagic vacuoles. Eur J Cell Biol 66:3鈥?4PubMed
  25.Smolen JS, Landewe R, Breedveld FC et al (2014) EULAR recommendations for the management of rheumatoid arthritis with synthetic and biological disease-modifying antirheumatic drugs: 2013 update. Ann Rheum Dis 73:492鈥?09PubMed Central PubMed View Article
  26.Hirsch G, Kitas G, Klocke R (2013) Intra-articular corticosteroid injection in osteoarthritis of the knee and hip: factors predicting pain relief鈥揳 systematic review. Semin Arthritis Rheum 42:451鈥?73PubMed View Article
  27.Ishikawa K (1981) Effect of intra-articular corticosteroid on the meniscus. A histological and histochemical study in rabbit knees. J Bone Joint Surg Am 63:120鈥?30PubMed
  28.Chrysis D, Ritzen EM, Savendahl L (2003) Growth retardation induced by dexamethasone is associated with increased apoptosis of the growth plate chondrocytes. J Endocrinol 176:331鈥?37PubMed View Article
  29.Annefeld M (1992) Changes in rat epiphyseal cartilage after treatment with dexamethasone and glycosaminoglycan-peptide complex. Pathol Res Pract 188:649鈥?52PubMed View Article
  30.Evans JF, Shen CL, Pollack S, Aloia JF, Yeh JK (2005) Adrenocorticotropin evokes transient elevations in intracellular free calcium ([Ca2+]i) and increases basal [Ca2+]i in resting chondrocytes through a phospholipase C-dependent mechanism. Endocrinology 146:3123鈥?132PubMed View Article
  31.Yao W, Dai W, Jiang JX, Lane NE (2013) Glucocorticoids and osteocyte autophagy. Bone 54:279鈥?84PubMed Central PubMed View Article
  32.Ivanova H, Vervliet T, Missiaen L, Parys JB, De Smedt H, Bultynck G (2014) Inositol 1,4,5-trisphosphate receptor-isoform diversity in cell death and survival. Biochimica et Biophysica Acta 1843(10):2164鈥?183PubMed View Article
  33.Luciani DS, Gwiazda KS, Yang TL et al (2009) Roles of IP3R and RyR Ca2+ channels in endoplasmic reticulum stress and beta-cell death. Diabetes 58:422鈥?32PubMed Central PubMed View Article
  34.Steinmann C, Landsverk ML, Barral JM, Boehning D (2008) Requirement of inositol 1,4,5-trisphosphate receptors for tumor-mediated lymphocyte apoptosis. J Biol Chem 283:13506鈥?3509PubMed Central PubMed View Article
  35.Criollo A, Vicencio JM, Tasdemir E, Maiuri MC, Lavandero S, Kroemer G (2007) The inositol trisphosphate receptor in the control of autophagy. Autophagy 3:350鈥?53PubMed View Article
  36.Harr MW, Rong Y, Bootman MD, Roderick HL, Distelhorst CW (2009) Glucocorticoid-mediated inhibition of Lck modulates the pattern of T cell receptor-induced calcium signals by down-regulating inositol 1,4,5-trisphosphate receptors. J Biol Chem 284:31860鈥?1871PubMed Central PubMed View Article
  37.Davis MC, McColl KS, Zhong F, Wang Z, Malone MH, Distelhorst CW (2008) Dexamethasone-induced inositol 1,4,5-trisphosphate receptor elevation in murine lymphoma cells is not required for dexamethasone-mediated calcium elevation and apoptosis. J Biol Chem 283:10357鈥?0365PubMed Central PubMed View Article
  
• 作者单位：Chao Shen (1)
  Wen Gu (2)
  Gui-Quan Cai (1)
  Jian-Ping Peng (1)
  Xiao-Dong Chen (1)
  
  1. Department of Orthopedic Surgery, Xinhua Hospital, 1665 Kongjiang Road, 200092, Shanghai, China
  2. Department of Geriatrics, Xinhua Hospital, 1665 Kongjiang Road, 200092, Shanghai, China
  
• 刊物类别：Medicine
• 刊物主题：Medicine & Public Health
  Oncology
  Cancer Research
  Cell Biology
  Biochemistry
  Virology
  
• 出版者：Springer Netherlands
• ISSN：1573-675X

文摘

Intra-articular injection of glucocorticoids (GCs) has been widely used in the management of osteoarthritis and rheumatoid arthritis. Nevertheless, several studies showed that GCs had toxic effects on chondrocytes as well as synovial cells. Previously we reported the protective role of autophagy in the degeneration of meniscal tissues. However, the effects of GCs on autophagy in the meniscal cells have not been fully elucidated. To investigate whether GCs can regulate autophagy in human meniscal cells, the meniscal cells were cultured in vitro and exposed in the presence of dexamethasone. The levels of apoptosis and autophagy were investigated via flow cytometry as well as western blotting analysis. The changes of the aggrecanases were measured using real-time PCR. The role of autophagy in dexamethasone-induced apoptosis was investigated using pharmacological agents and RNA interference technique. An agonist of inositol 1,4,5-trisphosphate receptor (IP3R) was used to investigate the mechanism of dexamethasone-induced autophagy. The results showed that dexamethasone induced autophagy as well as apoptosis in normal human meniscal cells. Using RNA interference technique and pharmacological agents, our results showed that autophagy protected the meniscal cells from dexamethasone-induced apoptosis. Our results also indicated that dexamethasone increased the mRNA levels of aggrecanases. This catabolic effect of dexamethasone was enhanced by 3-MA, the autophagy inhibitor. Furthermore, our results showed that dexamethasone induced autophagy via suppressing the phosphorylation of IP3R. In summary, our results indicated that autophagy protected meniscal cells from GCs-induced apoptosis via inositol trisphosphate receptor signaling.