The interplay between glucose and fatty acids on tube formation and fatty acid uptake in the first trimester trophoblast cells, HTR8/SVneo

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• 作者：Sanjay Basak ; Mrinal K. Das ; Vilasagar Srinivas…
• 关键词：Glucose ; Tube formation ; Fatty acid uptake ; First trimester trophoblast ; MMP9 ; Docosahexaenoic acid ; FABP4 ; HTR8/SVneo
• 刊名：Molecular and Cellular Biochemistry
• 出版年：2015
• 出版时间：March 2015
• 年：2015
• 卷：401
• 期：1-2
• 页码：11-19
• 全文大小：597 KB
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• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Biochemistry
  Medical Biochemistry
  Oncology
  Cardiology
  
• 出版者：Springer Netherlands
• ISSN：1573-4919

文摘

The study aims to investigate the impacts of hyperglycemia in the presence of fatty acids on early placentation processes that involves tube formation, cellular growth and proliferation, and metabolic activities of the first trimester trophoblast cells. Effects of maternal circulatory glucose levels that mimic physiological (5.5?mM), pre-diabetic (11?mM) and diabetic (?5?mM) phenotypes on tube formation (as a measure of angiogenesis in vitro), cellular viability and proliferation, fatty acid uptake and expression of genes associated with invasion, angiogenesis and fatty acid metabolism were examined using HTR8/SVneo cells. Glucose (25?mM) induced tube formation, viability, and proliferation of the first trimester trophoblast cells, HTR8/SVneo. Tube formation was, however, disintegrated in the presence of high glucose (40?mM) which was partially protected by eicosapentaenoic acid, 20:5n-3 (EPA) and docosahexaenoic acid, 22:6n-3 in vitro. Glucose (25?mM)-mediated induction in tube formation was favored by increased cellular uptake of [14C]EPA (p?mRNA and protein level of matrix metalloproteinase-9 (MMP9) (p?p?