Involvement of sulfates from cruzipain, a major antigen of Trypanosoma cruzi, in the interaction with immunomodulatory molecule Siglec-E

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• 作者：Maximiliano R. Ferrero ; Anja M. Heins…
• 关键词：Trypanosoma cruzi ; Siglecs ; Cruzipain ; Sialic acid ; Sulfates ; Immunomodulation
• 刊名：Medical Microbiology and Immunology
• 出版年：2016
• 出版时间：February 2016
• 年：2016
• 卷：205
• 期：1
• 页码：21-35
• 全文大小：2,449 KB
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• 作者单位：Maximiliano R. Ferrero (1)
  Anja M. Heins (2)
  Luciana L. Soprano (1)
  Diana M. Acosta (1)
  Mónica I. Esteva (1)
  Thomas Jacobs (2)
  Vilma G. Duschak (1)
  
  1. Área de Bioquímica de Proteínas y Glicobiología de Parásitos, Departamento de Investigación, Instituto Nacional de Parasitología “Dr. Mario Fatala Chaben”, ANLIS-Malbrán, Ministerio de Salud de la Nación, Av. Paseo Colón 568 (1063), Buenos Aires, Argentina
  2. Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Biomedicine
  Medical Microbiology
  Immunology
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1432-1831

文摘

In order to investigate the involvement of sulfated groups in the Trypanosoma cruzi host–parasite relationship, we studied the interaction between the major cysteine proteinase of T. cruzi, cruzipain (Cz), a sulfate-containing sialylated molecule and the sialic acid-binding immunoglobulin like lectin-E (Siglec-E). To this aim, ELISA, indirect immunofluorescence assays and flow cytometry, using mouse Siglec-E–Fc fusion molecules and glycoproteins of parasites, were performed. Competition assays verified that the lectins, Maackia amurensis II (Mal II) and Siglec-E–Fc, compete for the same binding sites. Taking into account that Mal II binding remains unaltered by sulfation, we established this lectin as sialylation degree control. Proteins of an enriched microsomal fraction showed the highest binding to Siglec-E as compared with those from the other parasite subcellular fractions. ELISA assays and the affinity purification of Cz by a Siglec-E column confirmed the interaction between both molecules. The significant decrease in binding of Siglec-E–Fc to Cz and to its C-terminal domain (C-T) after desulfation of these molecules suggests that sulfates contribute to the interaction between Siglec-E–Fc and these glycoproteins. Competitive ELISA assays confirmed the involvement of sulfated epitopes in the affinity between Siglec-E and Cz, probably modified by natural protein environment. Interestingly, data from flow cytometry of untreated and chlorate-treated parasites suggested that sulfates are not primary receptors, but enhance the binding of Siglec-E to trypomastigotic forms. Altogether, our findings support the notion that sulfate-containing sialylated glycoproteins interact with Siglec-E, an ortholog protein of human Siglec-9, and might modulate the immune response of the host, favoring parasitemia and persistence of the parasite. Keywords Trypanosoma cruzi Siglecs Cruzipain Sialic acid Sulfates Immunomodulation