Transmission electron microscopy study on the effects of the ultrasound contrast agent Levovist on hepatic cells

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• 作者：Etsuo Takada (1)
  Nobuki Kudo (2)
  Nobuyuki Endoh (3)
  Hiroyuki Hachiya (4)
  Shin-ichi Takeuchi (5)
  Takenobu Tsuchiya (3)
  Michiya Natori (6)
  
• 关键词：Microbubble ; Ultrasonic contrast agent ; Liver damage
• 刊名：Journal of Medical Ultrasonics
• 出版年：2012
• 出版时间：July 2012
• 年：2012
• 卷：39
• 期：3
• 页码：107-113
• 全文大小：1250KB
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  9. Shigeta K, Taniguchi N, Omoto K, Madoiwa S, Sakata Y, Mori M, Hatake K, Itoh K. In vitro platelet activation by an echo contrast agent. J Ultrasound Med. 2003;22:365-3.
  
• 作者单位：Etsuo Takada (1)
  Nobuki Kudo (2)
  Nobuyuki Endoh (3)
  Hiroyuki Hachiya (4)
  Shin-ichi Takeuchi (5)
  Takenobu Tsuchiya (3)
  Michiya Natori (6)
  
  1. Center of Medical Ultrasonics, Dokkyo Medical University School of Medicine, 880 Kitakobayashi, Mibu, Tochigi, 321-0293, Japan
  2. Laboratory of Biomedical Engineering, Graduate School of Information Science and Technology, Hokkaido University, Sapporo, Japan
  3. Department of Electronics and Informatics Frontiers, Faculty of Engineering, Kanagawa University, Yokohama, Japan
  4. Graduate School of Science and Engineering, Tokyo Institute of Technology, Tokyo, Japan
  5. Department of Clinical Engineering, Faculty of Biomedical Engineering, Toin University of Yokohama, Yokohama, Japan
  6. Research Institute, National Center for Child Health and Development, Tokyo, Japan
  

文摘

Purpose The Ultrasound Equipment and Safety Committee of The Japan Society of Ultrasonics in Medicine performed experiments to confirm whether contrast-enhanced ultrasonography damages liver cells. Methods Rats were injected with 0.1?ml of 300?mg/ml ultrasound contrast agent (UCA). Diagnostic ultrasound pulses with a center frequency of 6?MHz and a mechanical index of 1.9 were applied to rat livers with a water bag as a coupler to maintain a distance of 2-?cm between the ultrasound probe surface and the liver. Contrast-enhanced ultrasonography was carried out for 10?s to visualize the entire liver. Then, specimens of liver tissue were fixed using two types of fixation: immersion and perfusion fixation. Results Although some variations were found in electron micrographs of liver tissue fixed using immersion fixation, none of three blinded readers found any significant differences between micrographs of liver tissue from rats receiving UCA with sonication and those from sham-treated control rats. Changes observed were not thought to be group-specific but instead due to differences between individual rats. When the livers were fixed using perfusion fixation and the hepatic vein was cut after injection of physiological saline for perfusion, a large number of vacuoles ??μm in diameter were observed. This finding suggested that hepatic cell damage observed in this study was caused by high perfusion pressure during the liver fixation process rather than by sonication with UCA. Conclusion Blinded readings of electron micrographs showed no clear evidence that the use of Levovist in ADI mode ultrasonography causes significant damage to liver tissue.