Loss of the lac Operon Contributes to Salmonella Invasion of Epithelial Cells Through Derepression of Flagellar Synthesis

详细信息    查看全文

• 作者：Lingyan Jiang ; Zhiwei Ni ; Lei Wang ; Lu Feng ; Bin Liu
• 刊名：Current Microbiology
• 出版年：2015
• 出版时间：March 2015
• 年：2015
• 卷：70
• 期：3
• 页码：315-323
• 全文大小：388 KB
• 参考文献：1. Beltran P, Musser JM, Helmuth R, Farmer JR, Frerichs WM, Wachsmuth IK, Ferris K, McWhorter AC, Wells JG, Cravioto A, Et A (1988) Toward a population genetic analysis of / Salmonella: genetic diversity and relationships among strains of serotypes / S. choleraesuis, / S. derby, / S. dublin, / S. enteritidis, / S. heidelberg, / S. infantis, / S. newport, and / S. typhimurium. Proc Natl Acad Sci U S A 85:7753-757 CrossRef
  2. Bera A, Biswas R, Herbert S, Gotz F (2006) The presence of peptidoglycan O-acetyltransferase in various / staphylococcal species correlates with lysozyme resistance and pathogenicity. Infect Immun 74:4598-604 CrossRef
  3. Brenner FW, Villar RG, Angulo FJ, Tauxe R, Swaminathan B (2000) / Salmonella nomenclature. J Clin Microbiol 38:2465-467
  4. Briones V, Tellez S, Goyache J, Ballesteros C, Del PLM, Dominguez L, Fernandez-Garayzabal JF (2004) / Salmonella diversity associated with wild reptiles and amphibians in Spain. Environ Microbiol 6:868-71 CrossRef
  5. Chattopadhyay S, Paul S, Kisiela DI, Linardopoulou EV, Sokurenko EV (2012) Convergent molecular evolution of genomic cores in / Salmonella enterica and / Escherichia coli. J Bacteriol 194:5002-011 CrossRef
  6. Coburn B, Grassl GA, Finlay BB (2007) / Salmonella, the host and disease: a brief review. Immunol Cell Biol 85:112-18 CrossRef
  7. Crosa JH, Brenner DJ, Ewing WH, Falkow S (1973) Molecular relationships among the / Salmonelleae. J Bacteriol 115:307-15
  8. de Jong HK, Parry CM, van der Poll T, Wiersinga WJ (2012) Host-pathogen interaction in invasive salmonellosis. PLoS Pathog 8:e1002933 CrossRef
  9. Doolittle RF, Feng DF, Tsang S, Cho G, Little E (1996) Determining divergence times of the major kingdoms of living organisms with a protein clock. Science 271:470-77 CrossRef
  10. Eswarappa SM, Karnam G, Nagarajan AG, Chakraborty S, Chakravortty D (2009) / lac repressor is an antivirulence factor of / Salmonella enterica: its role in the evolution of virulence in / Salmonella. PLoS ONE 4:e5789 CrossRef
  11. Finlay BB, Brumell JH (2000) / Salmonella interactions with host cells: in vitro to in vivo. Philos Trans R Soc Lond B Biol Sci 355:623-31 CrossRef
  12. Fookes M, Schroeder GN, Langridge GC, Blondel CJ, Mammina C, Connor TR, Seth-Smith H, Vernikos GS, Robinson KS, Sanders M, Petty NK, Kingsley RA, Baumler AJ, Nuccio SP, Contreras I, Santiviago CA, Maskell D, Barrow P, Humphrey T, Nastasi A, Roberts M, Frankel G, Parkhill J, Dougan G, Thomson NR (2011) / Salmonella bongori provides insights into the evolution of the / Salmonellae. PLoS Pathog 7:e1002191 CrossRef
  13. Giannella RA, Washington O, Gemski P, Formal SB (1973) Invasion of HeLa cells by / Salmonella typhimurium: a model for study of invasiveness of / Salmonella. J Infect Dis 128:69-5 CrossRef
  14. Guise AJ, Budayeva HG, Diner BA, Cristea IM (2013) Histone deacetylases in herpesvirus replication and virus-stimulated host defense. Viruses 5:1607-632 CrossRef
  15. Hensel M (2004) Evolution of pathogenicity islands of / Salmonella enterica. Int J Med Microbiol 294:95-02 CrossRef
  16. Huang W, Wang Z, Lei QY (2014) Acetylation control of metabolic enzymes in cancer: an updated version. Acta Biochim Biophys Sin (Shanghai) 46:204-13 CrossRef
  
• 作者单位：Lingyan Jiang (1) (2)
  Zhiwei Ni (1) (2)
  Lei Wang (1) (2) (3) (4)
  Lu Feng (1) (2)
  Bin Liu (1) (2) (3)
  
  1. TEDA Institute of Biological Sciences and Biotechnology, Nankai University, Tianjin, 300457, People’s Republic of China
  2. The Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Tianjin, People’s Republic of China
  3. Tianjin Key Laboratory of Microbial Functional Genomics, Tianjin, People’s Republic of China
  4. State Key Laboratory of Medicinal Chemical Biology, Nankai University, Tianjin, People’s Republic of China
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Microbiology
  Biotechnology
  
• 出版者：Springer New York
• ISSN：1432-0991

文摘

Salmonella, a genus that is closely related to Escherichia coli, includes many pathogens of humans and other animals. A notable feature that distinguishes Salmonella from E. coli is lactose negativity, because the lac operon is lost in most Salmonella genomes. Here, we expressed the lac operon in Salmonella enterica serovar Typhimurium and compared the virulence of the Lac+ strain to that of the wild-type strain in a murine model, invasion assays, and macrophage replication assays. We showed that the Lac+ strain is attenuated in vivo and the attenuation of virulence is caused by its defect in epithelial cell invasion. However, the invasion-defective phenotype is unrelated to lactose utilization. Through sequencing and the comparison of the transcriptome profile between the Lac+ and wild-type strains during invasion, we found that most flagellar genes were?markedly downregulated in the Lac+ strain, while other genes associated with invasion, such as the majority of genes encoded in Salmonella pathogenicity island 1, were not differentially expressed. Moreover, we discovered that lacA is the major repressor of flagellar gene expression in the lac operon. In conclusion, these data demonstrate that the lac operon decreases Salmonella invasion of epithelial cells through repression of flagellar biosynthesis. As the ability to invade epithelial cells is a critical virulence determinant of Salmonella, our results provide important evidence that the loss of the lac operon contributes to the evolution of Salmonella pathogenicity.