Influence of inorganic phosphate on the production of free radicals in UV-irradiated frozen tyrosine solutions

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• 作者：T. A. Lozinova (1)
  A. V. Lander (2)
  
• 关键词：EPR ; tyrosine ; inorganic phosphate ; free radicals
• 刊名：Biophysics
• 出版年：2011
• 出版时间：August 2011
• 年：2011
• 卷：56
• 期：4
• 页码：590-595
• 全文大小：250KB
• 参考文献：1. E. N. Degtyarev, O. N. Brzhevskaya, and O. S. Nedelina, Dokl. RAN 410(4), 542 (2006).
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• 作者单位：T. A. Lozinova (1)
  A. V. Lander (2)
  
  1. Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, Moscow, 119334, Russia
  2. International Institute of Earthquake Prediction Theory and Mathematical Geophysics, Russian Academy of Sciences, Moscow, 117997, Russia
  
• ISSN：1555-6654

文摘

The role of inorganic phosphate as a catalyzer of the production of tyrosyl radical in frozen tyrosine solutions irradiated with near UV light at 77 K has been demonstrated by the EPR method. It was shown that the increase in the yield of tyrosyl radicals at pH < 7 correlates with the production of H-/sup> atoms and can be explained by the fact that phosphate acts as an acceptor of photoejected electrons. At pH > 7, the increase in the yield of tyrosyl radicals is accompanied by the production of phosphate radicals and OH-/sup> and is caused, presumably, by the catalysis of the formation of triplet states of tyrosine molecules by the HP form of phosphate, the fact shown by a number of authors. A quantitative estimation of relative concentrations of photosensitized paramagnetic products was carried out on the basis of computer analysis of resultant EPR signals.