Wild Birds, Frequent Carriers of Extended-Spectrum β-Lactamase (ESBL) Producing Escherichia coli of CTX-M and SHV-12 Types

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• 作者：Leticia Alcalá ; Carla Andrea Alonso ; Carmen Simón…
• 关键词：ESBL ; CMY ; 2 ; PMQR ; Escherichia coli ; Wild birds ; Spain
• 刊名：Microbial Ecology
• 出版年：2016
• 出版时间：November 2016
• 年：2016
• 卷：72
• 期：4
• 页码：861-869
• 全文大小：419 KB
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Microbiology
  Ecology
  Geoecology and Natural Processes
  Nature Conservation
  
• 出版者：Springer New York
• ISSN：1432-184X
• 卷排序：72

文摘

To get a better insight into the role of birds as reservoirs of extended-spectrum β-lactamase (ESBL) and plasmidic AmpC β-lactamase (pAmpC) Escherichia coli producers, 100 fecal samples belonging to 15 different wild avian species from Northern Spain were analyzed. Cefotaxime-resistant (CTXR) E. coli isolates were identified in 16 of the 100 tested birds, which corresponded to 9 animal species (Gyps fulvus—griffon vulture, Larus michahellis—yellow-legged gull, Milvus migrans—black kite, Milvus milvus—red kite, Ciconia ciconia—white stork, Sturnus unicolor—spotless starling, Aquila chrysaetos—golden eagle, Cuculus canorus—common cuckoo, Tyto alba—barn owl). Fifteen isolates harbored ESBL or pAmpC-encoding genes (number of isolates): bla_SHV-12 (9), bla_CTX-M-1 (3), bla_CTX-M-14 (2), and bla_CMY-2 (1). The last CTXR isolate presented a −42-point-mutation in the chromosomal ampC promoter. Eleven out of 15 ESBL/pAmpC E. coli isolates were multiresistant (most common resistance phenotype: β-lactams-quinolones-tetracycline-sulfamethoxazole/trimethoprim). A plasmid-mediated quinolone resistance determinant (qnrS1) was identified in one E. coli from a barn owl. High genetic diversity was observed among ESBL/pAmpC E. coli isolates, with 12 different sequence types (STs), including several strains of STs frequently detected among human clinical isolates (ST38/D, ST131/B2, ST155/B1, ST10/A). The ST131 isolate belonged to the emergent ciprofloxacin-resistant H30R subclone. This study reveals a high percentage of bird as carriers of ESBL/pAmpC E. coli isolates in Spain, highlighting the elevated rate among storks, kites, and vultures. Wild birds can contribute to the global spread of ESBL/pAmpC-producing E. coli in natural ecosystems.