Determination of UCP1 expression in subcutaneous and perirenal adipose tissues of patients with hypertension

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• 作者：Xueqin Li ; Juan Liu ; Gongcheng Wang ; Jing Yu ; Yunlu Sheng ; Chen Wang ; Yifan Lv…
• 关键词：Uncoupling protein 1 ; Perirenal adipose tissue ; Hypertension
• 刊名：Endocrine
• 出版年：2015
• 出版时间：November 2015
• 年：2015
• 卷：50
• 期：2
• 页码：413-423
• 全文大小：2,519 KB
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• 作者单位：Xueqin Li (1) (2)
  Juan Liu (1)
  Gongcheng Wang (3)
  Jing Yu (1)
  Yunlu Sheng (1)
  Chen Wang (1)
  Yifan Lv (1)
  Shan Lv (1)
  Hanmei Qi (1)
  Wenjuan Di (1)
  Changjun Yin (4)
  Guoxian Ding (1)
  
  1. Department of Geratology, The First Hospital Affiliated to Nanjing Medical University, 300 Guangzhou Road, Nanjing, 210029, People’s Republic of China
  2. Department of Geratology, Huai’an First People’s Hospital, Nanjing Medical University, 6 Beijing Road West, Huai’an, 223300, Jiangsu, People’s Republic of China
  3. Departments of Urology, Huai’an First People’s Hospital, Nanjing Medical University, 6 Beijing Road West, Huai’an, 223300, Jiangsu, People’s Republic of China
  4. Department of Urology, The First Hospital Affiliated to Nanjing Medical University, 300 Guangzhou Road, Nanjing, 210029, People’s Republic of China
  
• 刊物主题：Endocrinology; Diabetes; Internal Medicine; Science, general;
• 出版者：Springer US
• ISSN：1559-0100

文摘

The objective of this study is to determine the property of human perirenal adipose tissue (PAT) and assess the adipose property of PAT in hypertension. Ninety-four patients, including 64 normotensive patients (T-NP) and 30 hypertensive patients (HP), who underwent renal surgery were included. Expression analysis was performed using quantitative real-time polymerase chain reaction, Western blot, and immunohistochemistry in PAT and back subcutaneous adipose tissue (bSAT) depots. Compared with bSAT, PAT adipocytes were smaller, and the expressions of uncoupling protein-1 (UCP1) mRNA and protein were markedly higher, while the mRNA expressions of markers for classic beige and white adipocytes were lower in PAT. Immunohistochemistry analysis showed more multilocular UCP1-positive adipocytes in PAT than in bSAT. UCP1 expressions were lower in PAT in HP than in the T-NP or age- and body mass index-matched NP groups. Bigger unilocular adipocytes with less UCP1 staining in PAT were detected in HP than in NP group, although no such difference was observed in bSAT. PAT acts as a brown-like fat. UCP1 expression of PAT was lower in HP than in normotensive patients. UCP1 expression of PAT may serve as a protective indicator for hypertension.