A novel cyclic RGD-containing peptide polymer improves serum-free adhesion of adipose tissue-derived mesenchymal stem cells to bone implant surfaces
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- 作者：Péter Tátrai (1) (2)
Bernadett Sági (3)
Anna Szigeti (4)
áron Szepesi (4)
Ildikó Szabó (5)
Szilvia B?sze (5)
Zoltán Kristóf (6)
Károly Markó (7)
Gergely Szakács (1)
István Urbán (8)
Gábor Mez? (5)
Ferenc Uher (3)
Katalin Német (2) (4) - 刊名：Journal of Materials Science Materials in Medicine
- 出版年：2013
- 出版时间：February 2013
- 年：2013
- 卷：24
- 期：2
- 页码：479-488
- 全文大小：1280KB
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Bernadett Sági (3)
Anna Szigeti (4)
áron Szepesi (4)
Ildikó Szabó (5)
Szilvia B?sze (5)
Zoltán Kristóf (6)
Károly Markó (7)
Gergely Szakács (1)
István Urbán (8)
Gábor Mez? (5)
Ferenc Uher (3)
Katalin Német (2) (4)
1. Research Center for Natural Sciences, Hungarian Academy of Sciences, Budapest, Hungary
2. Department of Experimental Gene Therapy, National Blood Transfusion Service, Diószegi út 64, Budapest, 1113, Hungary
3. Stem Cell Laboratory, National Blood Transfusion Service, Budapest, Hungary
4. Creative Cell Ltd, Budapest, Hungary
5. Research Group of Peptide Chemistry, Hungarian Academy of Sciences, Budapest, Hungary
6. Department of Plant Anatomy, E?tv?s Loránd University, Budapest, Hungary
7. Institute of Experimental Medicine, Hungarian Academy of Sciences, Budapest, Hungary
8. Department of Restorative Dentistry, Loma Linda University, Loma Linda, CA, USA - ISSN：1573-4838
文摘
Seeding of bone implants with mesenchymal stem cells (MSCs) may promote osseointegration and bone regeneration. However, implant material surfaces, such as titanium or bovine bone mineral, fail to support rapid and efficient attachment of MSCs, especially under serum-free conditions that may be desirable when human applications or tightly controlled experiments are envisioned. Here we demonstrate that a branched poly[Lys(Seri-DL-Alam)] polymer functionalized with cyclic arginyl-glycyl-aspartate, when immobilized by simple adsorption to tissue culture plastic, surgical titanium alloy (Ti6Al4V), or Bio-Oss? bovine bone substitute, significantly accelerates serum-free adhesion and enhances seeding efficiency of human adipose tissue-derived MSCs. Moreover, when exposed to serum-containing osteogenic medium, MSCs survived and differentiated on the peptide-coated scaffolds. In summary, the presented novel polypeptide conjugate can be conveniently used for coating various surfaces, and may find applications whenever quick and efficient seeding of MSCs is required to various scaffolds in the absence of serum.