Cell viability study of thermo-responsive core–shell superparamagnetic nanoparticles for multimodal cancer therapy

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• 作者：Saqlain A. Shah (1)
  A. Majeed (2)
  M. A. Shafique (3)
  K. Rashid (4)
  Saif-Ullah Awan (5)
  
• 关键词：Biomaterials ; Magnetic materials ; Nanostructures ; Polymers
• 刊名：Applied Nanoscience
• 出版年：2014
• 出版时间：February 2014
• 年：2014
• 卷：4
• 期：2
• 页码：227-232
• 全文大小：363 KB
• 作者单位：Saqlain A. Shah (1)
  A. Majeed (2)
  M. A. Shafique (3)
  K. Rashid (4)
  Saif-Ullah Awan (5)
  
  1. Department of Physics, Forman Christian College (University), Lahore, Pakistan
  2. Department of Internal Medicine, Nazareth Hospital, Philadelphia, USA
  3. Center for Advanced Studies in Physics, GC University, Lahore, Pakistan
  4. Pakistan Council of Scientific and Industrial Research, Lahore, Pakistan
  5. Department of Physics, Quaid-i-Azam University, Islamabad, Pakistan
  
• ISSN：2190-5517

文摘

This is a vital extension of our previously published work. Thermo-responsive copolymer coated superparamagnetic MnFe2O4 nanoparticles are tested for cell viability and affinity on HeLa carcinoma cells under different conditions. Nanoparticles were loaded with anticancer drug doxorubicin. Composite nanoparticles of average diameter 45?nm were of core–shell structure having magnetic core of about 18?nm. Magnetic hyperthermia effects on cell viability and drug delivery were studied by exposing the cell suspension to high frequency magnetic field, and living cells were quantified using MTT method. There was almost absence of drug release at 37?°C. Drug was released at temperatures above lower critical solution temperature (LCST) by magnetic heating. LCST of the thermo-responsive copolymer was observed to be around 39?°C. Below this temperature, copolymer was hydrophilic and swelled. But above LCST, copolymer could become hydrophobic, expel water and drug and shrink in volume. Combination of hyperthermia and drug delivery effectively treated cancer cells.