Qualitative determination of bioactive metabolites through Q-TOF LC/MS in different parts and undifferentiated cultures of Ulmus wallichiana Planchon
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- 作者：Tejaswita Kumari ; Chetan Sharma ; Vikas Bajpai ; Brijesh Kumar…
- 关键词：Ulmus wallichiana ; Bone healing plant ; Endemic ; Uttarakhand ; Callus and cell suspension cultures ; Q ; TOF LC/MS
- 刊名：Plant Growth Regulation
- 出版年：2015
- 出版时间：January 2015
- 年：2015
- 卷：75
- 期：1
- 页码：331-340
- 全文大小：1,093 KB
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- 刊物主题：Life Sciences
Plant Physiology - 出版者：Springer Netherlands
- ISSN：1573-5087
文摘
Ulmus wallichiana is an endangered and folk medicinal plant used for healing fractures in India. Recently, the secondary metabolites isolated and identified from its bark revealed osteogenic activity. Considering the medicinal properties of its bark and the source for its identified and isolated metabolites, the requirement for production of alternative pathways was crucial. This study aimed to determine its bioactive metabolite using Quadrupole time-of-flight liquid chromatography-mass spectroscopy (Q-TOF LC/MS) in different parts of this plant with seasonal and altitudinal variability pertaining with the development of a protocol for induction of callus cultures and biomass production. Plant samples were collected during December–March, April–July and August–November in the year 2010-012 from three different altitudinal locations (Nainital, Almora and Bageshwar districts) of Kumaon region, Uttarakhand, India. The results obtained from Q-TOF LC/MS analysis demonstrated the presence of four bioactive metabolites known for osteogenic activity in bark and stem samples round the year. Syntheses of bioactive metabolites in leaf samples were significantly affected by sampling season as well as altitudinal locations respectively. Callus derived from leaf explants was maintained in Murashige and Skoog medium containing (0.25?mg?l?) 2,4-Dichlorophenoxy-acetic acid (2,4-D)?+?(0.05?mg?l?) Kinetin and (0.25?mg?l?) α-Naphthaleneacetic acid?+?(0.5?mg?l?) 6-Benzylaminopurine in liquid phase showed optimal conditions for accumulating biomass and synthesis of identified bioactive metabolites.