Immobilization of active lipase B from Candida antarctica on the surface of polyhydroxyalkanoate inclusions

详细信息    查看全文

• 作者：Anika C. Jahns (1) (2) (4)
  Bernd H. A. Rehm (1) (2) (3)
  
  1. Institute of Fundamental Sciences ; Massey University ; Private Bag 11222 ; Palmerston North ; 4442 ; New Zealand
  2. PolyBatics Ltd ; BioCommerce Centre ; Dairy Farm Road ; Palmerston North ; 4442 ; New Zealand
  4. Department of Medical Biosciences ; Ume氓 University ; 90185 ; Ume氓 ; Sweden
  3. MacDiarmid Institute for Advanced Materials and Nanotechnology ; Kelburn Parade ; Wellington ; 6140 ; New Zealand
  
• 关键词：Beads ; Lipase ; PHA synthase (PhaC) ; Polyhydroxyalkanoate beads
• 刊名：Biotechnology Letters
• 出版年：2015
• 出版时间：April 2015
• 年：2015
• 卷：37
• 期：4
• 页码：831-835
• 全文大小：552 KB
• 参考文献：1. Chen, B, Hu, J, Miller, EM, Xie, W, Cai, M, Gross, RA (2008) Candida antarctica lipase B chemically immobilized on epoxy-activated micro鈥攁nd nanobeads: catalysts for polyester synthesis. Biomacromolecules 9: pp. 463-471 CrossRef
  2. Goodman, LP, Dugan, LR (1969) The effect of sonication on lipase activity. Lipids 5: pp. 362-365 CrossRef
  3. Grage, K, Jahns, AC, Parlane, N, Palanisamy, R, Rasiah, IA, Atwood, JA, Rehm, BHA (2009) Bacterial polyhydroxyalkanoate granules: biogenesis, structure, and potential use as nano-/micro-beads in biotechnological and biomedical applications. Biomacromolecules 10: pp. 660-669 CrossRef
  4. Jaeger, K-E, Reetz, MT (1998) Microbial lipases form versatile tools for biotechnology. Trends Biotechnol 16: pp. 396-403 CrossRef
  5. Jahns, AC, Haverkamp, RG, Rehm, BHA (2008) Multifunctional inorganic-binding beads self-assembled inside engineered bacteria. Bioconjug Chem 19: pp. 2072-2080 CrossRef
  6. Mateo, C, Palomo, JM, Fernandez-Lorente, G, Guisan, JM, Ferndandez-Lafuente, R (2007) Improvement of enzyme activity, stability and selectivity via immobilization techniques. Enzyme Microb Technol 40: pp. 1451-1463 CrossRef
  7. Mei, Y, Miller, L, Gao, W, Gross, RA (2003) Imaging the distribution and secondary structure of immobilized enzymes using infrared microspectroscopy. Biomacromolecules 4: pp. 70-74 CrossRef
  8. Rathi, P, Saxena, RK, Gupta, R (2001) A novel alkaline lipase from Burkholderia cepacia for detergent formulation. Process Biochem 37: pp. 187-192 CrossRef
  9. Rehm, BHA (2003) Polyester synthases: natural catalysts for plastics. Biochem J 376: pp. 15-33 CrossRef
  10. Rehm, BHA (2006) Genetics and biochemistry of polyhydroxyalkanoate granule self-assembly: the key role of polyester synthases. Biotechnol Lett 28: pp. 207-213 CrossRef
  11. Sheldon, RA (2007) Enzyme immobilization: the quest for optimum performance. Adv Synth Catal 349: pp. 1289-1307 CrossRef
  12. Svendsen, A, Clausen, IG, Patkar, SA, Borch, K, Thellersen, M (1997) Protein engineering of microbial lipases of industrial interest. Methods Enzymol 284: pp. 317-340 CrossRef
  13. Tan, T, Hu, J, Nie, K, Deng, L, Wang, F (2010) Biodiesel production with immobilized lipase: a review. Biotechnol Adv 28: pp. 628-634 CrossRef
  14. Uppenberg, J, Hansen, MT, Patkar, S, Jones, TA (1994) The sequence, crystal structure determination and refinement of two crystal forms of lipase B from Candida antarctica. Sequence 2: pp. 293-308
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Microbiology
  Biotechnology
  Applied Microbiology
  Biochemistry
  
• 出版者：Springer Netherlands
• ISSN：1573-6776

文摘

Polyhydroxyalkanoate (PHA) beads, recombinantly produced in Escherichia coli, were functionalized to display lipase B from Candida antarctica as translational protein fusion. The respective beads were characterized in respect to protein content, functionality, long term storage capacity and re-usability. The direct fusion of the PHA synthase, PhaC, to lipase B yielded active PHA lipase beads capable of hydrolyzing glycerol tributyrate. Lipase B beads showed stable activity over several weeks and re-usability without loss of function.