Reactive Oxygen Species (ROS) in human semen: determination of a reference range

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• 作者：Sheryl T. Homa ; Wayne Vessey…
• 关键词：Reactive oxygen species ; Oxidative stress ; Chemiluminescence ; Human semen ; Male infertility
• 刊名：Journal of Assisted Reproduction and Genetics
• 出版年：2015
• 出版时间：May 2015
• 年：2015
• 卷：32
• 期：5
• 页码：757-764
• 全文大小：337 KB
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• 作者单位：Sheryl T. Homa (1) (2)
  Wayne Vessey (1)
  Ana Perez-Miranda (1)
  Tripat Riyait (1)
  Ashok Agarwal (3)
  
  1. Andrology Department, The Doctors Laboratory, London, W1T 4EU, UK
  2. Andrology Solutions, London, W1G 9RT, UK
  3. Andrology Center, Cleveland Clinic, Cleveland, OH, 44195, USA
  
• 刊物类别：Medicine
• 刊物主题：Medicine & Public Health
  Gynecology
  Developmental Biology
  Human Genetics
  
• 出版者：Springer Netherlands
• ISSN：1573-7330

文摘

Purpose High levels of reactive oxygen species (ROS) are a leading cause of male factor infertility. Measurement of ROS has been hampered by a lack of standardisation and confounding variables including choice of controls and sample selection. This study aimed to determine a reference range for ROS in human semen. Methods Semen samples were obtained from men attending for routine semen analysis who gave informed consent for the study. Samples were assigned groups: Group 1 (N--4) normal semen parameters, no leucocytospermia; Group 2 (N--00) abnormal semen parameters, no leucocytospermia; Group 3 (N--1) any semen parameters with leucocytospermia. ROS levels were assayed in fresh neat semen using a chemiluminescence assay measured in a single tube luminometer. Data are reported in relative light units (RLU)/sec/106 sperm Results ROS levels were significantly different between Groups 1, 2 and 3 (19.75?±-.12, 95.03?±-3.63, 890.17?±-10.23 RLU/sec/106 sperm respectively; p-lt;-.001). Group 3 gave the highest value confirming this group as the optimum choice for positive controls. The reference range-lt;-4.1 RLU/sec/106 sperm was determined by ROC analysis that differentiates a reference population (Group 1) from a positive control group (Group 3), optimising the sensitivity and specificity (80.5 and 87.2?% respectively) of the test. Conclusions We have determined a reference range for ROS in human semen and identified a patient population that falls outside the normal range. This simple, cost effective assay can be incorporated into routine diagnostic testing to aid in the diagnosis of male infertility, especially with regard to unexplained infertility.