Identification and in silico Analysis of NADPH Oxidase Homologues Involved in Allergy from an Olive Pollen Transcriptome

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• 作者：Mar铆a Jos茅 Jim茅nez-Quesada (20)
  Jose 脕ngel Traverso (20) (22)
  Adoraci贸n Zafra (20)
  Jos茅 C. Jimenez-Lopez (20)
  Rosario Carmona (20) (21)
  M. Gonzalo Claros (21)
  Juan de Dios Alch茅 (20)
  
  20. Estaci贸n Experimental del Zaid铆n (CSIC) ; Granada ; Spain
  22. Departamento Biolog铆a Celular ; Universidad de Granada ; Granada ; Spain
  21. Departamento de Biolog铆a Molecular y Bioqu铆mica ; Universidad de M谩laga ; M谩laga ; Spain
  
• 关键词：allergy ; NADPH oxidase ; pollen ; Rboh ; ROS ; superoxide
• 刊名：Lecture Notes in Computer Science
• 出版年：2015
• 出版时间：2015
• 年：2015
• 卷：9043
• 期：1
• 页码：450-459
• 全文大小：376 KB
• 参考文献：1. Liccardi, G., D鈥橝mato, M., D鈥橝mato, G. (1996) Oleaceae pollinosis: a review. International Archives of Allergy and Immunology 111: pp. 210-217 CrossRef
  2. Villalba, M., Rodriguez, R., Batanero, E.: The spectrum of olive pollen allergens. From structures to diagnosis and treatment. Methods (San Diego, Calif.) 66, 44鈥?4 (2014)
  3. Speranza, A., Scoccianti, V. (2012) New insights into an old story: pollen ROS also play a role in hay fever. Plant Signal Behav. 7: pp. 994-998 CrossRef
  4. Boldogh, I., Bacsi, A., Choudhury, B.K., Dharajiya, N., Alam, R., Hazra, T.K., Mitra, S., Goldblum, R.M., Sur, S. (2005) ROS generated by pollen NADPH oxidase provide a signal that augments antigen-induced allergic airway inflammation. J. Clin. Invest. 115: pp. 2169-2179 CrossRef
  5. Dharajiya, N., Boldogh, I., Cardenas, V., Sur, S. (2008) Role of pollen NAD(P)H oxidase in allergic inflammation. Current Opinion in Allergy and Clinical Immunology 8: pp. 57-62 CrossRef
  6. Bright, J., Hiscock, S.J., James, P.E., Hancock, J.T. (2009) Pollen generates nitric oxide and nitrite: a possible link to pollen-induced allergic responses. Plant Physiol. Biochem. 47: pp. 49-55 CrossRef
  7. Wang, X.L., Takai, T., Kamijo, S., Gunawan, H., Ogawa, H., Okumura, K. (2009) NADPH oxidase activity in allergenic pollen grains of different plant species. Biochem. Biophys. Res. Commun. 387: pp. 430-434 CrossRef
  8. Lamb, C., Dixon, R.A. (1997) The oxidative burst in plant disease resistance. Annual Review of Plant Physiology and Plant Molecular Biology 48: pp. 251-275 CrossRef
  9. Lambeth, J.D. (2004) NOX enzymes and the biology of reactive oxygen. Nature Reviews. Immunology 4: pp. 181-189 CrossRef
  10. Bedard, K., Lardy, B., Krause, K.H. (2007) NOX family NADPH oxidases: not just in mammals. Biochimie 89: pp. 1107-1112 CrossRef
  11. Dangl, J.L., Jones, J.D. (2001) Plant pathogens and integrated defence responses to infection. Nature 411: pp. 826-833 CrossRef
  12. Torres, M.A., Onouchi, H., Hamada, S., Machida, C., Hammond-Kosack, K.E., Jones, J.D. (1998) Six Arabidopsis thaliana homologues of the human respiratory burst oxidase (gp91phox). Plant J. 14: pp. 365-370 CrossRef
  13. Sagi, M., Fluhr, R. (2006) Production of reactive oxygen species by plant NADPH oxidases. Plant Physiol. 141: pp. 336-340 CrossRef
  14. Kaya, H., Nakajima, R., Iwano, M., Kanaoka, M.M., Kimura, S., Takeda, S., Kawarazaki, T., Senzaki, E., Hamamura, Y., Higashiyama, T., Takayama, S., Abe, M., Kuchitsu, K.: Ca2+-activated Reactive Oxygen Species production by Arabidopsis RbohH and RbohJ is essential for proper pollen tube tip growth. The Plant Cell Online (2014)
  15. Lassig, R., Gutermuth, T., Bey, T.D., Konrad, K.R., Romeis, T.: Pollen tube NAD(P)H oxidases act as a speed control to dampen growth rate oscillations during polarized cell growth. The Plant Journal (2014)
  16. Pazmandi, K., Kumar, B.V., Szabo, K., Boldogh, I., Szoor, A., Vereb, G., Veres, A., Lanyi, A., Rajnavolgyi, E., Bacsi, A.: Ragweed Subpollen Particles of Respirable Size Activate Human Dendritic Cells. PLoS ONE 7, e52085 (2012)
  17. Bacsi, A., Dharajiya, N., Choudhury, B.K., Sur, S., Boldogh, I. (2005) Effect of pollen-mediated oxidative stress on immediate hypersensitivity reactions and late-phase inflammation in allergic conjunctivitis. The Journal of Allergy and Clinical Immunology 116: pp. 836-843 CrossRef
  18. Shalaby, K.H., Allard-Coutu, A., O鈥橲ullivan, M.J., Nakada, E., Qureshi, S.T., Day, B.J., Martin, J.G. (2013) Inhaled birch pollen extract induces airway hyperresponsiveness via oxidative stress but independently of pollen-intrinsic NADPH oxidase activity, or the TLR4-TRIF pathway. Journal of Immunology 191: pp. 922-933 CrossRef
  19. Alch茅, J.D., M鈥檙ani-Alaoui, M., Castro, A.J., Rodr铆guez-Garc铆a, M.I.: Ole e 1, the major allergen from olive (Olea europaea L.) pollen, increases its expression and is released to the culture medium during in vitro germination. Plant and Cell Physiology 45,1149鈥?157 (2004)
  20. McWilliam, H., Li, W., Uludag, M., Squizzato, S., Park, Y.M., Buso, N., Cowley, A.P., Lopez, R.: Analysis Tool Web Services from the EMBL-EBI. Nucleic Acids Research 41,W597鈥揥600 (2013)
  21. Gouy, M., Guindon, S., Gascuel, O. (2010) SeaView Version 4: A Multiplatform Graphical User Interface for Sequence Alignment and Phylogenetic Tree Building. Molecular Biology and Evolution 27: pp. 221-224 CrossRef
  22. Darriba, D., Taboada, G.L., Doallo, R., Posada, D. (2012) jModelTest 2: more models, new heuristics and parallel computing. Nat. Meth. 9: pp. 772-772 CrossRef
  23. Chou, K.C., Shen, H.B.: Plant-mPLoc: a top-down strategy to augment the power for predicting plant protein subcellular localization. PLoS One 5,e11335 (2010)
  24. Heazlewood, J.L., Durek, P., Hummel, J., Selbig, J., Weckwerth, W., Walther, D., Schulze, W.X.: PhosPhAt: a database of phosphorylation sites in Arabidopsis thaliana and a plant-specific phosphorylation site predictor. Nucleic Acids Research 36, D1015鈥揇1021 (2008)
  25. Lee, T.-Y., Bretana, N., Lu, C.-T.: PlantPhos: using maximal dependence decomposition to identify plant phosphorylation sites with substrate site specificity. BMC Bioinformatics 12, 261 (2011)
  26. Xue, Y., Liu, Z., Gao, X., Jin, C., Wen, L., Yao, X., Ren, J.: GPS-SNO: Computational Prediction of Protein S-Nitrosylation Sites with a Modified GPS Algorithm. PLoS ONE 5, e11290 (2010)
  27. Martinez, A., Traverso, J.A., Valot, B., Ferro, M., Espagne, C., Ephritikhine, G., Zivy, M., Giglione, C., Meinnel, T.: Extent of N-terminal modifications in cytosolic proteins from eukaryotes. Proteomics 8, 2809鈥?831 (2008)
  28. Kelley, L.A., Sternberg, M.J.: Protein structure prediction on the Web: a case study using the Phyre server. Nature Protocols 4, 363鈥?71 (2009)
  29. Oda, T., Hashimoto, H., Kuwabara, N., Akashi, S., Hayashi, K., Kojima, C., Wong, H.L., Kawasaki, T., Shimamoto, K., Sato, M., Shimizu, T. (2010) Structure of the N-terminal regulatory domain of a plant NADPH oxidase and its functional implications. J. Biol. Chem. 285: pp. 1435-1445 CrossRef
  30. Wass, M.N., Kelley, L.A., Sternberg, M.J.: 3DLigandSite: predicting ligand-binding sites using similar structures. Nucleic Acids Research 38,W469鈥揥473 (2010)
  31. Boisson-Dernier, A., Lituiev, D.S., Nestorova, A., Franck, C.M., Thirugnanarajah, S., Grossniklaus, U.: ANXUR receptor-like kinases coordinate cell wall integrity with growth at the pollen tube tip via NADPH oxidases. PLoS Biology 11, e1001719 (2013)
  32. Potocky, M., Jones, M.A., Bezvoda, R., Smirnoff, N., Zarsky, V.: Reactive oxygen species produced by NADPH oxidase are involved in pollen tube growth. New Phytol. 174, 742鈥?51 (2007)
  33. Potocky, M., Pejchar, P., Gutkowska, M., Jimenez-Quesada, M.J., Potocka, A., Alche Jde, D., Kost, B., Zarsky, V.: NADPH oxidase activity in pollen tubes is affected by calcium ions, signaling phospholipids and Rac/Rop GTPases. J. Plant Physiol. 169, 1654鈥?663 (2012)
  34. Zafra, A., Rodriguez-Garcia, M.I., Alch茅, J.D.: Cellular localization of ROS and NO in olive reproductive tissues during flower development. BMC Plant Biol. 10, 36 (2010)
  35. Yun, B.W., Feechan, A., Yin, M., Saidi, N.B., Le Bihan, T., Yu, M., Moore, J.W., Kang, J.G., Kwon, E., Spoel, S.H., Pallas, J.A., Loake, G.J.: S-nitrosylation of NADPH oxidase regulates cell death in plant immunity. Nature 478, 264鈥?68 (2011)
  36. Drerup, M.M., Schlucking, K., Hashimoto, K., Manishankar, P., Steinhorst, L., Kuchitsu, K., Kudla, J.: The calcineurin B-like calcium sensors CBL1 and CBL9 together with their interacting protein kinase CIPK26 regulate the Arabidopsis NADPH oxidase RBOHF. Mol. Plant (2013)
  37. Ogasawara, Y., Kaya, H., Hiraoka, G., Yumoto, F., Kimura, S., Kadota, Y., Hishinuma, H., Senzaki, E., Yamagoe, S., Nagata, K., Nara, M., Suzuki, K., Tanokura, M., Kuchitsu, K.: Synergistic activation of the Arabidopsis NADPH oxidase AtrbohD by Ca2+ and phosphorylation. J. Biol. Chem. 283, 8885鈥?892 (2008)
  
• 作者单位：Bioinformatics and Biomedical Engineering
• 丛书名：978-3-319-16482-3
• 刊物类别：Computer Science
• 刊物主题：Artificial Intelligence and Robotics
  Computer Communication Networks
  Software Engineering
  Data Encryption
  Database Management
  Computation by Abstract Devices
  Algorithm Analysis and Problem Complexity
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1611-3349

文摘

Reactive oxygen species generated by pollen NADPH oxidases are present in numerous allergenic pollen species. The superoxide generated by this enzyme has been suggested as a key actor in the induction of allergic inflammation. However, this enzyme has been characterized in Arabidopsis thaliana pollen only, where two pollen-specific genes (RbohH and RbohJ) have been described. The olive (Olea europaea L.) pollen is an important source of allergy in Mediterranean countries. We have assembled and annotated an olive pollen transcriptome, which allowed us to determine the presence of at least two pollen-specific NADPH oxidase homologues. Primers were designed to distinguish between the two homologues, and full-length sequences were obtained through a PCR strategy. Complete in silico analysis of such sequences, including phylogeny, 3-D modeling of the N-terminus, and prediction of cellular localization and post-translational modifications was carried out with the purpose of shed light into the involvement of olive pollen-intrinsic NADPH oxidases in triggering allergy symptoms.