Identification of Distinctive Variants of the Olive Pollen Allergen Ole e 5 (Cu,Zn Superoxide Dismutase) throughout the Analysis of the Olive Pollen Transcriptome

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• 作者：Adoraci贸n Zafra (20)
  Rosario Carmona (20) (21)
  Jos茅 C. Jimenez-Lopez (20)
  Amada Pulido (22)
  M. Gonzalo Claros (21)
  Juan de Dios Alch茅 (20)
  
  20. Estaci贸n Experimental del Zaid铆n (CSIC) ; Granada ; Spain
  21. Departamento de Biolog铆a Molecular y Bioqu铆mica ; Universidad de M谩laga ; M谩laga ; Spain
  22. Departamento de Fisiolog铆a Vegetal ; Universidad de Granada ; Granada ; Spain
  
• 关键词：allergen ; deletion ; Ole e 5 ; pollen ; Superoxide Dismutase
• 刊名：Lecture Notes in Computer Science
• 出版年：2015
• 出版时间：2015
• 年：2015
• 卷：9043
• 期：1
• 页码：460-470
• 全文大小：1,480 KB
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• 作者单位：Bioinformatics and Biomedical Engineering
• 丛书名：978-3-319-16482-3
• 刊物类别：Computer Science
• 刊物主题：Artificial Intelligence and Robotics
  Computer Communication Networks
  Software Engineering
  Data Encryption
  Database Management
  Computation by Abstract Devices
  Algorithm Analysis and Problem Complexity
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1611-3349

文摘

Ole e 5 is an olive pollen allergen displaying high identity with Cu,Zn superoxide dismutases. Previous studies characterized biochemical variability in this allergen, which may be of relevance for allergy diagnosis and therapy. The generation of an olive pollen transcriptome allowed us to identify eight Ole e 5 sequences, one of them including a 24 nt deletion. Further in silico analysis permitted designing primers for PCR amplification and cloning from both cDNA and gDNA. A large number of sequences were retrieved, which experimentally validated the predictive NGS sequences, including the deleted enzyme. The PCR-obtained sequences were used for further scrutiny, including sequence aligment and phylogenetic analysis. Two model sequences (a complete sequence and a deleted one) were used to perform 3-D modeling and a prediction of the T- and B-cell epitopes. These predictions interestingly foreseed relevant differences in the antigenicity/allergenicity of both molecules. Clinical relevance of differences is discussed.