Biodistribution and PET Imaging of a Novel [68Ga]-Anti-CD163-Antibody Conjugate in Rats with Collagen-Induced Arthritis and in Controls
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- 作者：Sascha Eichendorff (1)
Pia Svendsen (1)
Dirk Bender (2)
Susanne Keiding (2) (3)
Erik I. Christensen (1)
Bent Deleuran (1)
S酶ren K. Moestrup (1) (4)
1. Department of Biomedicine ; Aarhus University ; Ole Worms Alle 3 ; Blng 1170 ; 8000 ; Aarhus ; Denmark
2. Department of Nuclear Medicine & PET Centre ; Aarhus University Hospital ; Aarhus ; Denmark
3. Department of Hepatology and Gastroenterology ; Aarhus University Hospital ; Aarhus ; Denmark
4. Department of Clinical Biochemistry ; Aarhus University Hospital ; Aarhus ; Denmark - 关键词：CD163 ; Positron emission tomography ; Arthritis ; Macrophages ; Liver
- 刊名：Molecular Imaging and Biology
- 出版年：2015
- 出版时间：February 2015
- 年：2015
- 卷：17
- 期：1
- 页码：87-93
- 全文大小：1,142 KB
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- 出版者：Springer US
- ISSN：1860-2002
文摘
Purpose The hemoglobin scavenger receptor CD163 is exclusively expressed on monocytes and tissue macrophages, also at sites of inflammation. We examined whether gallium-68 (Ga-68)-labeled anti-CD163-antibody can detect the receptor in vivo. Procedures We radiolabeled anti-CD163 antibody with Ga-68 and evaluated stability and binding specificity of the conjugate ([68Ga]ED2) in vitro. Furthermore, tracer biodistribution was assessed in vivo in healthy rats and rats with acute collagen-induced arthritis (CIA) by MicroPET and tissue analysis. Results Radiosynthesis of [68Ga]ED2 antibody yielded a tracer with high-specific activity and radiochemical purity. [68Ga]ED2 bound specifically to CD163 in vitro. In rats, [68Ga]ED2 rapidly accumulated in macrophage-rich tissues. The arthritic paws exhibited a low but significant [68Ga]ED2 uptake. Interestingly, the systemic distribution was also changed in the sense that a significantly higher liver uptake and lower spleen uptake of [68Ga]ED2 was measured in CIA rats that accordingly showed a corresponding change in level of CD163 expression. Conclusions [68Ga]ED2 specifically binds CD163 in vitro and in vivo. Biodistribution studies in CIA rats suggest that this novel tool may have applications in studies of inflammatory diseases.