GhDRIN1, a novel drought-induced gene of upland cotton (Gossypium hirsutum L.) confers abiotic and biotic stress tolerance in transgenic tobacco

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• 作者：Gurusamy Dhandapani (1) (2)
  Azhagiyamanavalan Lakshmi Prabha (2)
  Mogilicherla Kanakachari (1) (2)
  Mullapudi Lakshmi Venkata Phanindra (1)
  Narayanasamy Prabhakaran (1) (2)
  Sellamuthu Gothandapani (1)
  Kethireddy Venkata Padmalatha (1)
  Amolkumar U. Solanke (1)
  Polumetla Ananda Kumar (1) (3)
  
  1. National Research Centre on Plant Biotechnology ; LBS Building ; Pusa Campus ; New Delhi ; 110012 ; India
  2. Department of Plant Science ; Bharathidasan University ; Tiruchirappalli ; 620024 ; Tamil Nadu ; India
  3. Institute of Biotechnology ; Acharya N. G. Ranga Agricultural University ; Rajendranagar ; Hyderabad ; 500030 ; Telangana ; India
  
• 关键词：Drought ; induced ; Fungal pathogens ; Gossypium hirsutum ; Helicoverpa armigera ; Salt stress ; Transgenic tobacco ; Water ; deficit stress
• 刊名：Biotechnology Letters
• 出版年：2015
• 出版时间：April 2015
• 年：2015
• 卷：37
• 期：4
• 页码：907-919
• 全文大小：1,358 KB
• 参考文献：1. Artico, S, Nardeli, SM, Brilhante, O, Grossi-de-Sa, MF, Alves-Ferreira, M (2010) Identification and evaluation of new reference genes in Gossypium hirsutum for accurate normalization of real-time quantitative RT-PCR data. BMC Plant Biol 10: pp. 49 CrossRef
  2. Bartels, D, Sunkar, R (2005) Drought and salt tolerance in plants. Crit Rev Plant Sci 24: pp. 23-58 CrossRef
  3. Dhandapani G, Kanakachari M, Padmalatha KV, Phanindra MLV, Singh VK, Raghavendrarao S, Jayabalan N, Lakshmi Prabha A, Kumar PA (2014) A gene encoding cold-circadian rhythm-RNA binding-like protein ( / CCR-Like) from upland cotton ( / Gossypium hirsutum L.) confers tolerance to abiotic stresses in transgenic tobacco. Plant Mol Biol Rep. doi: 10.1007/s11105-014-0729-x
  4. Dowd, C, Wilson, IW, McFadden, H (2004) Gene expression profile changes in cotton root and hypocotyl tissues in response to infection with Fusarium oxysporum f. sp. vasinfectum. Mol Plant Microb Interact 17: pp. 654-667 CrossRef
  5. Gollery, M, Harper, J, Cushman, J, Mittler, T, Girke, T, Zhu, JK, Bailey-Serres, J, Mittler, R (2006) What makes species unique? The contribution of proteins with obscure features. Genome Biol 7: pp. 757 CrossRef
  6. Gollery, M, Harper, J, Cushman, J, Mittler, T, Mittler, R (2007) POFs: what we don鈥檛 know can hurt us. Trends Plant Sci 12: pp. 492-496 CrossRef
  7. Gupta, GP, Birah, A, Ravi, S (2004) Development of artificial diet for mass rearing of American bollworms (Helicoverpa armigera). Indian J Agric Sci 74: pp. 548-557
  8. Hirayama, T, Shinozaki, K (2010) Research on plant abiotic stress responses in the post-genome era: past, present and future. Plant J 61: pp. 1041-1052 CrossRef
  9. Horan, K, Jang, C, Bailey-Serres, J, Mittler, R, Shelton, C, Harper, JF, Zhu, JK, Cushman, JC, Gollery, M, Girke, T (2008) Annotating genes of known and unknown function by large-scale coexpression analysis. Plant Physiol 147: pp. 41-57 CrossRef
  10. Luhua, S, Ciftci-Yilmaz, S, Harper, J, Cushman, J, Mittler, R (2008) Enhanced tolerance to oxidative stress in transgenic arabidopsis plants expressing proteins of unknown function. Plant Physiol 148: pp. 280-292 CrossRef
  11. Luhua, S, Hegie, A, Suzuki, N, Shulaev, E, Luo, X, Cenariu, D, Ma, V, Kao, S, Lim, J, Gunay, MB, Oosumi, T, Lee, SC, Harper, J, Cushman, J, Gollery, M, Girke, T, Bailey-Serres, J, Stevenson, RA, Zhu, JK, Mittler, R (2013) Linking genes of unknown function with abiotic stress responses by high-throughput phenotype screening. Physiol Plant 148: pp. 322-333 CrossRef
  12. Padmalatha, KV, Dhandapani, G, Kanakachari, M, Kumar, S, Dass, A, Patil, DP, Rajamani, V, Kumar, K, Pathak, R, Rawat, B, Leelavathi, S, Reddy, PS, Jain, N, Powar, KN, Hiremath, V, Katageri, IS, Reddy, MK, Solanke, AU, Reddy, VS, Kumar, PA (2012) Genome-wide transcriptomic analysis of cotton under drought stress reveal significant down-regulation of genes and pathways involved in fibre elongation and up-regulation of defense responsive genes. Plant Mol Biol 78: pp. 223-246 CrossRef
  13. Wang, B, Luttge, U, Ratajczak, R (2001) Effects of salt treatment and osmotic stress on V-ATPase and V-PPase in leaves of the halophyte Suaeda salsa. J Exp Bot 52: pp. 2355-2365 CrossRef
  14. Zhang, L, Li, FG, Liu, CL, Zhang, CJ, Zhang, XY (2009) Construction and analysis of cotton (Gossypium arboreum L.) drought-related cDNA library. BMC Res Notes 2: pp. 120 CrossRef
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Microbiology
  Biotechnology
  Applied Microbiology
  Biochemistry
  
• 出版者：Springer Netherlands
• ISSN：1573-6776

文摘

A novel stress tolerance cDNA fragment encoding GhDRIN1 protein was identified and its regulation was studied in cotton boll tissues and seedlings subjected to various biotic and abiotic stresses. Phylogenetic and conserved domain prediction indicated that GhDRIN1 was annotated with a hypothetical protein of unknown function. Subcellular localization showed that GhDRIN1 is localized in the chloroplasts. The promoter sequence was isolated and subjected to in silico study. Various cis-acting elements responsive to biotic and abiotic stresses and hormones were found. Transgenic tobacco seedlings exhibited better growth on amended MS medium and showed minimal leaf damage in insect bioassays carried out with Helicoverpa armigera larvae. Transgenic tobacco showed better tolerance to water-deficit and fast recovered upon rewatering. Present work demonstrated that GhDRIN1, a novel stress tolerance gene of cotton, positively regulates the response to biotic and abiotic stresses in transgenic tobacco.