Nano-size uni-lamellar lipodisq improved in situ auto-phosphorylation analysis of E. coli tyrosine kinase using 19F nuclear magnetic resonance
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  • 作者:Dong Li (1)
    Juan Li (1)
    Yonglong Zhuang (3)
    Longhua Zhang (1)
    Ying Xiong (1)
    Pan Shi (2)
    Changlin Tian (1) (2)

    1. Hefei National Laboratory for Physical Science at the Microscale & School of Life Science
    ; University of Science and Technology of China ; Hefei ; 230026 ; China
    3. Department of Chemistry
    ; Anhui University ; Hefei ; 230027 ; China
    2. High Magnetic Field Laboratory
    ; Chinese Academy of Sciences ; Hefei ; 230031 ; China
  • 刊名:Protein & Cell
  • 出版年:2015
  • 出版时间:March 2015
  • 年:2015
  • 卷:6
  • 期:3
  • 页码:229-233
  • 全文大小:423 KB
  • 参考文献:1. Barnes, CO, Pielak, GJ (2011) In-cell protein NMR and protein leakage. Proteins 79: pp. 347-351 CrossRef
    2. Bayburt, TH, Sligar, SG (2003) Self-assembly of single integral membrane proteins into soluble nanoscale phospholipid bilayers. Protein Sci 12: pp. 2476-2481 CrossRef
    3. Bayburt, TH, Sligar, SG (2010) Membrane protein assembly into Nanodiscs. FEBS Lett 584: pp. 1721-1727 CrossRef
    4. Hammill, JT, Miyake-Stoner, S, Hazen, JL, Jackson, JC, Mehl, RA (2007) Preparation of site-specifically labeled fluorinated proteins for 19F-NMR structural characterization. Nat Protoc 2: pp. 2601-2607 CrossRef
    5. Hunter, T (2009) Tyrosine phosphorylation: thirty years and counting. Curr Opin Cell Biol 21: pp. 140-146 CrossRef
    6. Jackson, JC, Hammill, JT, Mehl, RA (2007) Site-specific incorporation of a (19) F-amino acid into proteins as an NMR probe for characterizing protein structure and reactivity. J Am Chem Soc 129: pp. 1160-1166 CrossRef
    7. Johnson, H, White, FM (2012) Toward quantitative phosphotyrosine profiling in vivo. Semin Cell Dev Biol 23: pp. 854-862 CrossRef
    8. Knowles, TJ, Finka, R, Smith, C, Lin, YP, Dafforn, T, Overduin, M (2009) Membrane proteins solubilized intact in lipid containing nanoparticles bounded by styrene maleic acid copolymer. J Am Chem Soc 131: pp. 7484-7485 CrossRef
    9. Lee, DC, Zheng, J, She, YM, Jia, Z (2008) Structure of Escherichia coli tyrosine kinase Etk reveals a novel activation mechanism. EMBO J 27: pp. 1758-1766 CrossRef
    10. Li, F, Shi, P, Li, J, Yang, F, Wang, T, Zhang, W, Gao, F, Ding, W, Li, D, Li, J (2013) A genetically encoded 19F NMR probe for tyrosine phosphorylation. Angew Chem Int Ed Engl 52: pp. 3958-3962 CrossRef
    11. Orwick, MC, Judge, PJ, Procek, J, Lindholm, L, Graziadei, A, Engel, A, Grobner, G, Watts, A (2012) Detergent-free formation and physicochemical characterization of nanosized lipid-polymer complexes: Lipodisq. Angew Chem Int Ed Engl 51: pp. 4653-4657 CrossRef
    12. Orwick-Rydmark, M, Lovett, JE, Graziadei, A, Lindholm, L, Hicks, MR, Watts, A (2012) Detergent-Free incorporation of a seven-transmembrane receptor protein into nanosized bilayer Lipodisq particles for functional and biophysical studies. Nano lett 12: pp. 4687-4692 CrossRef
    13. Raschle, T, Hiller, S, Etzkorn, M, Wagner, G (2010) Nonmicellar systems for solution NMR spectroscopy of membrane proteins. Curr Opin Struct Biol 20: pp. 471-479 CrossRef
    14. Shi, P, Xi, Z, Wang, H, Shi, C, Xiong, Y, Tian, C (2010) Site-specific protein backbone and side-chain NMR chemical shift and relaxation analysis of human vinexin SH3 domain using a genetically encoded 15N/19F-labeled unnatural amino acid. Biochem Biophys Res Commun 402: pp. 461-466 CrossRef
    15. Shi, P, Wang, H, Xi, Z, Shi, C, Xiong, Y, Tian, C (2011) Site-specific (1)(9)F NMR chemical shift and side chain relaxation analysis of a membrane protein labeled with an unnatural amino acid. Protein Sci 20: pp. 224-228 CrossRef
    16. Shi, P, Li, D, Chen, H, Xiong, Y, Wang, Y, Tian, C (2012) In situ 19F NMR studies of an E. coli membrane protein. Protein Sci 21: pp. 596-600 CrossRef
  • 刊物主题:Biochemistry, general; Protein Science; Cell Biology; Stem Cells; Human Genetics; Developmental Biology;
  • 出版者:Springer Berlin Heidelberg
  • ISSN:1674-8018
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