Lipid and fatty acid profiles of Pseudomonas aurantiaca DNA-bound lipids determined by mass spectrometry

详细信息    查看全文

• 作者：R. I. Zhdanov (1) (2)
  D. Kern (3)
  W. Lorenz (3)
  M. Y. Ibragimova (1)
  
  1. Kazan (Volga region) Federal University ; Kazan ; Russia
  2. Sholokhov Moscow State University for Humanities ; Russian Institute for Advanced Study in Humanities and Technology ; Moscow ; 109240 ; Russia
  3. Martin Luther University of Halle-Wittenberg ; Wittenberg ; Germany
  
• 关键词：Pseudomonas aurantiaca ; DNA ; bound lipids ; fatty acids ; mass spectrometry ; electrospray ionization
• 刊名：Microbiology
• 出版年：2015
• 出版时间：January 2015
• 年：2015
• 卷：84
• 期：1
• 页码：34-40
• 全文大小：291 KB
• 参考文献：1. Ivanova, P., Milne, S., and Byrne M.O., Glycerophospholipid identification and quantitation by electrospray mass spectrometry, / Methods Enzymol., 2007, vol. 432, pp. 21鈥?7. CrossRef
  2. Bischoff, G. and Zhdanov, R.I. Lipidomics鈥攍ipids take part in complexity of human genome, / Chem. Ingen. Tech. 2002, vol. 74, no. 5, p. 714. CrossRef
  3. Lagarde, M., Geloen, A., Record, M., Vance, D., and Spenner, F., Lipidomics is emerging, / Biochim. Biophys. Acta, 2003, vol. 1634, p. 61. CrossRef
  4. Wenk, M.R., The emerging field of lipidomics, / Nature Rev., 2005, vol. 4, pp. 594鈥?10.
  5. Zhdanov, R.I., Salafutdinov, I.I., Arslan, A., and Ibragimova, M.Ya., New marker of FAME profile of / Pseudomonas aurantiaca total lipids, / Doklady Biochem. Biophys., 2012, vol. 445, no. 1, pp. 183鈥?86. CrossRef
  6. Tarasov, D.S., Ibragimova, M.Ya., Izotova, E.D., Akberova, N.I. and Zhdanov, R.I., Molecular dynamics and free energy of binding of oleic acid to DNA in aqueous solutions, / Doklady Biochem. Biophys., 2012, vol. 446, no. 2, pp. 223鈥?28. CrossRef
  7. Zhdanov, R.I., Krylov, A.S., Zarubina, T.V., Bishoff, G., and Ibragimova, M.Y., Complexation between DNA and cardiolipin according spectrophotometry and circular dichroism data, / Doklady Biochem. Biophys., 2013, vol. 453, no. 3, pp. 290鈥?94.
  8. Carvalho, M., Sampaio, J.L., Palm, W., Brankatschk, M., Eaton, S., and Shevchenko, A., Effects of diet and development on the / Drosophila lipidome, / J. Mol. Syst. Biol., 2012, vol. 8, no. 1, pp. 600鈥?05.
  9. Struchkov, V.A., Strazhevskaya, N.B., and Zhdanov, R.I., Specific natural DNA-bound lipids in postgenome era. Lipid conception of chromatin organization, / Bioelectrochem., 2002, vol. 56, pp. 195鈥?98. CrossRef
  10. Struchkov, V.A., Strazhevskaya, N.B., and Zhdanov, R.I., DNA-bound lipids of normal and tumor cells: retrospective and outlooks for functional genomics and lipid-DNA code, / Bioelectrochem., 2002, vol. 58, pp. 23鈥?1. CrossRef
  11. Albi, E. and Viola Magni, M.P., The role of intranuclear lipids, / Biol. Cell., 2004, vol. 96, no. 8, pp. 657鈥?67. CrossRef
  12. Hannun, Y.A. and Obeid, L.M., Principles of bioactive lipid signaling: lessons from sphingolipids, / Nat. Rev. Mol. Cell Biol., 2008, vol. 9, no. 2, pp. 139鈥?50. CrossRef
  13. Jump, D.B., Fatty acid regulation of gene transcription, / Crit. Rev. Clin. Lab. Sci., 2004, vol. 41, no. 1, pp. 41鈥?8. CrossRef
  14. Zhdanov, R.I., Strazhevskaya, N.B., Jdanov, A.R., and Bischoff, G., A spectroscopic and surface plasmon resonance study of oleic acid/DNA complexes, / J. Biomol. Str. Dyn., 2002, vol. 20, no. 2, pp. 231鈥?41. CrossRef
  15. Zhdanov, R.I., Bishoff, G., and Ibragimova, M.Y., On association of DNA polynucleotides with cardiolipin, / Uch. Zap. Kazan. Univ. Ser. Nat. Sci., 2012, vol. 154, no. 4, pp. 164鈥?74.
  16. Zhdanov, R.I., Struchkov, V.A., Dyabina, O.V., and Strazhevskaya, N.B., Chromatin-bound cardiolipin: the phospholipid of prolifaretion, / Cytobios, 2001, vol. 106, pp. 55鈥?1.
  17. Zhdanov, R.I., Shmyrina, A.S., Zarubina, T.V., Kraus, A., and Lorenz, W., Fatty acid profiles of DNA-bound and whole-cell lipids of / Pseudomonas aurantiaca drastically differ, / Doklady Biochem. Biophys., 2006, vol. 410, no. 4, pp. 292鈥?96. CrossRef
  18. Zhdanov, R.I., Shmyrina, A.S., Mulyukin, A.L., El鈥橰egistan, G.I., Zarubina, T.V., Kraus, A., Haupt, N., and Lorenz, W., The lipid fraction tightly bound to genomic DNA is determined in prokaryotes, / Doklady Biochem. Biophys., 2006, vol. 410, no. 6, pp. 320鈥?23. CrossRef
  19. Zhdanov, R., Shmyrina, A., Zarubina, T., Mulyukin, A., El-Registan, G., Haupt, N., Kraus, A., and Lorenz, W., The nature of / P. aurantiaca DNA-bound lipids, / FEMS Microbiol. Lett. 2006, vol. 265, pp. 151鈥?58. CrossRef
  20. Zhdanov, R.I.m Kurbanov, R.A., and Ibragimova, M.Y., RF Patent no. RU 2506314, 2013.
  21. Struchkov, V.A. and Strazhevskaya, N.B., DNA-bound lipids: composition and possible function, / Biokhimiya, 1993, vol. 58, no. 8, pp. 1154鈥?175.
  22. Shepelev, A.P., Shestopalov, A.V., Ryndich, A.A., Staviskii, I.M., and Yagovkina, V.V., DNA-bound lipids of thermoresistant and thermolabile / Shigella sonnei strains, / Bull. Eksp. Biol. Med., 1995, vol. 120, no. 2, pp. 332鈥?33.
  23. Mulyukin, A.L., Vakhrushev, M.A., Strazhevskaya, N.B., Shmyrina, A.S., Zhdanov, R.I., Suzina, N.E., Duda, V.I., and El鈥?Registan, G.I., Effect of alkylhydroxybenzenes, microbial anabiosis inducers, on the structural organization of / Pseudomo- nas aurantiaca DNA and on the Induction of phenotypic dissociation, / Microbiology (Moscow). 2005, vol. 74, no. 2, pp. 128鈥?35. CrossRef
  24. Strazhevskaya, N.B., Mulyukin, A.L., Shmyrina, A.S., Kraus, A., Lorenz, V., Zhdanov, R.I., and El鈥橰egistan, G.I., Characteristics of / Pseudomonas aurantiaca DNA supramolecular complexes at various developmental stages, / Microbiology (Moscow), 2009, vol. 78, no. 1, pp. 48鈥?5. CrossRef
  25. Zhdanov, R.I. and Ibragimova, M.Y., New informational level at genomic DNA: lipids specifically bound to DNA, / Genetics and Chemistry Sharing a Language of Discovery: Cell Symposium Series, Boston, 2013, p. 29.
  26. Moder, M., Braun, P., Lange, F., Schrader, S., and Lorenz, W., Determination of endocrine disrupting compounds and acidic drugs in water by coupling of derivatization, gas chromatography and negativechemical ionization mass spectrometry, / Clean-Soil, Air, Water, 2007, vol. 35, no. 5, pp. 444鈥?51. CrossRef
  27. Abb, M., Heinrich, T., Sorkau, E., and Lorenz, W., Phthalates in house dust, / Environ. Int., 2009, vol. 35, no. 6, pp. 965鈥?70. CrossRef
  28. Ruiz N., Kahne D., and Silhavy T.J., Advances in understanding bacterial outer-membrane biogenesis, / Nature Rev. Microbiol. 2006, vol. 4, pp. 57鈥?6. CrossRef
  29. D鈥檡achkov, E.P., Ibragimova, M.Y., D鈥檡achkov, P.N., and Zhdanov, R.I., Investigation of DNA interaction with fatty acids by molecular docking, / Uch. Zap. Kazan. Univ. Ser. Nat. Sci., 2011, vol. 153, no. 1, pp. 86鈥?6.
  30. Fuchs, B., Suess, R., and Schuller, Ju., An up-date of MALDI-TOF mass spectrometry in lipid research, / Progr. Lipid Res., 2010, vol. 49, pp. 450鈥?75. CrossRef
  31. Zhdanov, R.I. and Kubatiev, A.A., Lipidomics and sciences of human genome. An additional information level exists in genomic DNA: lipids, / Patogenez, 2003, vol. 1, no. 1, pp. 7鈥?0.
  32. Strazhevskaya, N.B., Zhdanov, R.I., and Kubatiev, A.A., Structural lipidomics. DNA-bound lipids of euchromatin, heterochromatin, and nuclear matrix of rat thymus and liver, / Patogenez, 2004, vol. 2, no. 1, pp. 4鈥?.
  33. Manzoli, F.A., Capitani, S., Mazzotti, G., Barnabei, O., and Maraldi, N.M., Role of chromatin phospholipids on template availability and ultrastructure of isolated nuclei, / Adv. Enzyme Reg., 1982, vol. 20, pp. 247鈥?62. CrossRef
  34. Maraldi, N.M., Caramelli, E., Capitani, S., Marinelli, F., Antonucci, A., Mazzotti, G., and Manzoli, F.A., Threedimensional analysis of the interphase nucleus, / Biol. Cell., 1982, vol. 46, pp. 325鈥?28.
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Microbiology
  Medical Microbiology
  Russian Library of Science
  
• 出版者：MAIK Nauka/Interperiodica distributed exclusively by Springer Science+Business Media LLC.
• ISSN：1608-3237

文摘

An approach used on investigation of the lipid composition of loosely (fraction 1) and tightly (fraction 2) DNA-bound lipids of Pseudomonas aurantiaca cells by electrospray ionization using mass spectrometry (ESI-LC-MS) was used for determination of the lipidome of a prokaryotic cell. Free fatty acids C16:0, C18:1 (fraction 1), C14:0, C16:0, and C18:2 (fraction 2) were detected. Both fractions of DNA-bound lipids were characterized by the presence of phosphatidylglycerol, phosphatidylserine, and lyso-phosphatidylinositol. The alcohol-soluble fraction 1 could also contain phosphatidylcholine and phosphatidylinositol, while fraction 2 probably contained triacylglycerides. Compared to gas chromatography, ESI-LC-MS provides new possibilities for investigation of the nucleoid lipidome, allowing for more detailed investigation of DNA-bound lipids in bacterial cells.