PBMC and exosome-derived Hotair is a critical regulator and potent marker for rheumatoid arthritis

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• 作者：Jinsoo Song (1)
  Dongkyun Kim (1)
  Jiyeon Han (1)
  Yunha Kim (1)
  Myeungsu Lee (2)
  Eun-Jung Jin (1)
  
  1. Department of Biological Sciences ; College of Natural Sciences ; Wonkwang University ; Iksan ; Chunbuk ; 570-749 ; Korea
  2. Division Rheumatology ; Department of Internal Medicine ; School of Medicine ; Wonkwang University ; Iksan ; 570-749 ; Korea
  
• 关键词：LncRNA ; Hotair ; BMCS ; Exosome ; Rheumatoid arthritis ; Matrix metalloprotease
• 刊名：Clinical and Experimental Medicine
• 出版年：2015
• 出版时间：February 2015
• 年：2015
• 卷：15
• 期：1
• 页码：121-126
• 全文大小：1,115 KB
• 参考文献：1. Rhodes B, Merriman ME, Harrison A, Nissen MJ, Smith M, Stamp L, Steer S, Merriman TR, Vyse TJ (2010) A genetic association study of serum acute-phase C-reactive protein levels in rheumatoid arthritis: implications for clinical interpretation. PLoS Med 7:e1000341 CrossRef
  2. Wapinski O, Chang HY (2011) Long noncoding RNAs and human disease. Trends Cell Biol 21:354鈥?61 CrossRef
  3. Mattick JS, Taft RJ, Faulkner GJ (2010) A global view of genomic information鈥搈oving beyond the gene and the master regulator. Trends Genet 26:21鈥?8 CrossRef
  4. Lee C, Kikyo N (2012) Strategies to identify long noncoding RNAs involved in gene regulation. Cell Biosci 6(2):37 CrossRef
  5. Stuhlm眉ller B, Kunisch E, Franz J, Martinez-Gamboa L, Hernandez MM, Pruss A, Ulbrich N, Erdmann VA, Burmester GR, Kinne RW (2003) Detection of oncofetal h19 RNA in rheumatoid arthritis synovial tissue. Am J Pathol 163:901鈥?11 CrossRef
  6. Steck E, Boeuf S, Gabler J, Werth N, Schnatzer P, Diederichs S, Richter W (2010) Regulation of H19 and its encoded microRNA-675 in osteoarthritis and under anabolic and catabolic in vitro conditions. J Mol Med 90:1185鈥?195 CrossRef
  7. Denzer K, Kleijmeer MJ, Heijnen HF, Stoorvogel W, Geuze HJ (2000) Exosome: from internal vesicle of the multivesicular body to intercellular signaling device. J Cell Sci 113(Pt 19):3365鈥?374
  8. Keller S, Sanderson MP, Stoeck A, Altevogt P (2006) Exosomes: from biogenesis and secretion to biological function. Immunol Lett 107:102鈥?08 CrossRef
  9. Pauli A, Rinn JL, Schier AF (2011) Non-coding RNAs as regulators of embryogenesis. Nat Rev Genet 12:136鈥?49 CrossRef
  10. Guttman M, Amit I, Garber M, French C, Lin MF, Feldser D, Huarte M, Zuk O, Carey BW, Cassady JP, Cabili MN, Jaenisch R, Mikkelsen TS, Jacks T, Hacohen N, Bernstein BE, Kellis M, Regev A, Rinn JL, Lander ES (2009) Chromatin signature reveals over a thousand highly conserved large non-coding RNAs in mammals. Nature 458:223鈥?27 CrossRef
  11. Rinn JL, Kertesz M, Wang JK, Squazzo SL, Xu X, Brugmann SA, Goodnough LH, Helms JA, Farnham PJ, Segal E, Chang HY (2007) Functional demarcation of active and silent chromatin domains in human HOX loci by noncoding RNAs. Cell 129:1311鈥?323 CrossRef
  12. Kim K, Jutooru I, Chadalapaka G, Johnson G, Frank J, Burghardt R, Kim S, Safe S (2013) HOTAIR is a negative prognostic factor and exhibits pro-oncogenic activity in pancreatic cancer. Oncogene 32:1616鈥?625 CrossRef
  13. Gupta SK, Bang C, Thum T (2010) Circulating microRNAs as biomarkers and potential paracrine mediators of cardiovascular disease. Circ Cardiovasc Genet 3:484鈥?88 CrossRef
  14. Th茅ry C, Duban L, Segura E, V茅ron P, Lantz O, Amigorena S (2002) Indirect activation of na茂ve CD4+ T cells by dendritic cell-derived exosomes. Nat Immunol 3:1156鈥?162 CrossRef
  15. Lin R, Wang S, Zhao RC (2013) Exosomes from human adipose-derived mesenchymal stem cells promote migration through Wnt signaling pathway in a breast cancer cell model. Mol Cell Biochem 383:13鈥?0
  
• 刊物主题：Internal Medicine; Hematology; Oncology;
• 出版者：Springer Milan
• ISSN：1591-9528

文摘

Despite growing importance of long non-coding RNAs (lncRNAs) in normal physiological and disease conditions, our knowledge of RA-related lncRNAs remains limited. Therefore, we aimed to identify lncRNA signatures that have prognostic values in RA. There was a notably high expression level of Hotair in blood mononuclear cells and serum exosome of rheumatoid arthritis (RA) patients, leading the migration of active macrophage. In contrast, markedly lower level of Hotair was detected in differentiated osteoclasts and rheumatoid synoviocytes and enforced expression of Hotair led to significantly decreased levels of MMP-2 and MMP-13. This exploratory study provides novel empirical evidence that Hotair could be one of potential biomarkers for diagnosing RA.