Functional Validation of Phragmites communis Glutathione Reductase (PhaGR) as an Essential Enzyme in Salt Tolerance

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• 作者：Xia Zhang (1)
  Geng Quan (1)
  Jing Wang (1)
  Huiling Han (1)
  ShiHua Chen (1)
  ShanLi Guo (1)
  HaiBo Yin (1)
  
  1. Key Laboratory of Plant Molecular & Developmental Biology ; College of Life Sciences ; Yantai University ; Yantai ; 264005 ; Shandong ; China
  
• 关键词：Glutathione reductase ; Reed ; Salt ; Reduced glutathione (GSH)
• 刊名：Applied Biochemistry and Biotechnology
• 出版年：2015
• 出版时间：April 2015
• 年：2015
• 卷：175
• 期：7
• 页码：3418-3430
• 全文大小：1,878 KB
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• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Biotechnology
  Biochemistry
  
• 出版者：Humana Press Inc.
• ISSN：1559-0291

文摘

Reed plants (Phragmites communis (Linn.) Trin) are hydrophilic perennial grasses growing in fresh and brackish waters. These plants readily adapt to arid and high salinity conditions; however, their resistance mechanism against abiotic stresses, especially high salinity, is largely unknown. In the present study, we cloned a glutathione reductase gene from P. communis and investigated its role in conferring salt tolerance in reed plants. The expression of PhaGR at the transcriptional level was affected by multiple abiotic stresses including NaCl, Cd2+, heat, cold, PEG 6000, and abscisic acid (ABA). Furthermore, NaCl and Cd2+ could increase its expressions at the translational level. NaCl and Cd2+ also increased the biosynthesis of soluble protein and reduced glutathione (GSH). Reed seedlings that were challenged with NaCl showed higher levels of GR activities, which corroborated our gene expression data. The increase in GR possibly increased the salt tolerance of reed plants through GSH production. Thus, PhaGR is a potential target gene in improving the salt tolerance of crops through genetic manipulation.