The MAT1-1:MAT1-2 Ratio of Sporothrix globosa Isolates in Japan

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• 作者：Rui Kano (1)
  Clement K.-M. Tsui (2)
  Richard C. Hamelin (2)
  Kazushi Anzawa (3)
  Takashi Mochizuki (3)
  Katsutaro Nishimoto (4)
  Masataro Hiruma (5)
  Hiroshi Kamata (1)
  Atsuhiko Hasegawa (6)
  
  1. Department of Pathobiology ; Nihon University School of Veterinary Medicine ; 1866 Kameino ; Fujisawa ; Kanagawa ; 252-8510 ; Japan
  2. Department of Forest and Conservation Sciences ; University of British Columbia ; Vancouver ; BC ; V6T 1Z4 ; Canada
  3. Department of Dermatology ; Kanazawa Medical University ; Uchinada ; Ishikawa ; 920-0293 ; Japan
  4. Division of Dermatology ; Ekisaikai Nagasaki Hospital ; 5-16 Kabashimacho ; Nagasaki-City ; Nagasaki ; 850-0034 ; Japan
  5. Ochanomizu Institute for Medical Mycology and Allergology ; Nakamura Bldg. 2F ; 2-12-4 Hongo ; Bunkyoku ; Tokyo ; 113-0033 ; Japan
  6. Teikyo University Institute of Medical Mycology ; 359 Otsuka ; Hachioji ; Tokyo ; 192-0395 ; Japan
  
• 关键词：Mating type ; MAT1 ; 1 ; 1 ; Sporothrix globosa ; Sporotrichosis ; Sexual reproduction
• 刊名：Mycopathologia
• 出版年：2015
• 出版时间：February 2015
• 年：2015
• 卷：179
• 期：1-2
• 页码：81-86
• 全文大小：251 KB
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• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Microbiology
  Medical Microbiology
  Plant Sciences
  Microbial Ecology
  
• 出版者：Springer Netherlands
• ISSN：1573-0832

文摘

In order to understand the reproductive biology of pathogenic species in the Sporothrix schenckii complex, we characterized the partial mating type (MAT1-1) loci of Sporothrix schenckii, as well as the S. globosa MAT1-1-1 gene, which encoded 262 amino acid sequences. The data confirmed that the MAT1-1 locus of S. globosa was divergent from the MAT1-2 locus of the opposite mating type, suggesting that the fungus is heterothallic. To determine the mating type ratio of 20 isolates from Japanese patients, we analyzed the MAT loci by specific PCR amplification of MAT1-1-1 and MAT1-2-1 genes. The MAT1-1-1 was detected in 5 isolates but not in the other 15 isolates with the presence of MAT1-2-1. The MAT1-1:1-2 ratio of S. globosa isolates in Japan was estimated to be 1:3. Phylogenetic analysis indicated that the sequences of the MAT1-1-1 were identical among S. globosa isolates but different from S. schenckii and Ophiostoma montium.