Molecular genetic analysis of microorganisms with intraepithelial invasion isolated from patients with colorectal cancer

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• 作者：N. T. Nguyen ; R. R. Vafin ; I. V. Rzhanova…
• 刊名：Molecular Genetics, Microbiology and Virology
• 出版年：2016
• 出版时间：January 2016
• 年：2016
• 卷：31
• 期：1
• 页码：15-20
• 全文大小：642 KB
• 刊物主题：Microbiology; Molecular Medicine;
• 出版者：Springer US
• ISSN：1934-841X
• 卷排序：31

文摘

The facultative aerobic bacteria isolated from the mucosa of rectum in the zone of malignant tumor and neighboring normal mucosa in patients with colorectal cancer were studied using molecular genetic methods. The species attribution of bacteria was implemented using the cultural morphological analysis and sequencing of the 16S rRNA locus. The microorganisms with intraepithelial invasion to rectal mucosa were identified as the representatives of adherent-invasive (AIEC) subgroup of Escherichia coli and species Klebsiella pneumonia. The molecular analysis by genetic determinants controlling adhesive, hemolytic, and toxigenic activity revealed that some bacterial isolates were able to produce toxins with potential cancerogenic activity (e.g., colibactin and cytotoxic necrotizing factor 1). Certain bacterial species isolated from malignant and normal rectal epithelium of the same patient demonstrated no difference between analyzed factors of toxigenicity.Keywordscolorectal cancerEscherichia coliKlebsiella pneumoniacolibactincytotoxic necrotizing factor 1identificationPCRsequencing