Direct Chiral HPLC Method for the Simultaneous Determination of Warfarin Enantiomers and Its Impurities in Raw Material and Pharmaceutical Formulation: Application of Chemometric Protocol
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- 作者：Kannappan Valliappan (1)
Sree Janardhanan Vaithiyanathan (1)
Venkatesan Palanivel (1) - 关键词：Column liquid chromatography ; Chiral stationary phase ; Chiral separation ; Derringer’s desirability function ; Multicriteria decision ; making approach ; Warfarin
- 刊名：Chromatographia
- 出版年：2013
- 出版时间：6 - March 2013
- 年：2013
- 卷：76
- 期：5
- 页码：287-292
- 全文大小：303KB
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Sree Janardhanan Vaithiyanathan (1)
Venkatesan Palanivel (1)
1. Department of Pharmacy, Annamalai University, Annamalai Nagar, Chidambaram, Tamil Nadu, 608002, India - ISSN：1612-1112
文摘
Warfarin is a well-known anticoagulant agent that occurs in two enantiomers, (R)-(+)-warfarin and (S)-(?-warfarin, in which 4-hydroxycoumarin and benzalacetone are commonly found as impurities. Due to the lack of analytical reports for the simultaneous estimation on warfarin and its impurities in bulk drug and pharmaceuticals, we aim at the simultaneous estimation and optimization of the chromatographic separation of warfarin and its related substances employing experimental design. Central composite design was employed to evaluate the influence of two independent variables (concentration of organic modifier and flow rate) on the output responses: capacity factor (k 1), resolution of the peak (Rs 3,4), and retention time of the last peak (tR 5), as well as to model these responses. Further, the central composite design results were combined in a multicriteria decision-making approach in order to obtain a set of optimal experimental conditions leading to the most desirable compromise between resolution and analysis time.