A newly discovered arabinoxylan-specific arabinofuranohydrolase. Synergistic action with xylanases from different glycosyl hydrolase families
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- 作者：Adrià Valls ; Pilar Diaz ; F. I. Javier Pastor…
- 关键词：Arabinoxylan ; Bioconversion ; Enzymatic hydrolysis ; GH43 ; synergism
- 刊名：Applied Microbiology and Biotechnology
- 出版年：2016
- 出版时间：February 2016
- 年：2016
- 卷：100
- 期：4
- 页码：1743-1751
- 全文大小：833 KB
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Pilar Diaz (1)
F. I. Javier Pastor (1)
Susana V. Valenzuela (1)
1. Department of Microbiology, Faculty of Biology, University of Barcelona, Av. Diagonal 643, 08028, Barcelona, Spain - 刊物类别：Chemistry and Materials Science
- 刊物主题：Chemistry
Biotechnology
Microbiology
Microbial Genetics and Genomics - 出版者：Springer Berlin / Heidelberg
- ISSN：1432-0614
文摘
Arabinofuranosidase Abf43A from Bacillus sp. BP-7 is a newly discovered arabinoxylan arabinofuranohydrolase (AXH). It is a modular enzyme comprised of a GH43 catalytic domain and a carbohydrate-binding module of family CBM6. Recombinant Abf43A showed high activity on arabinoxylans, being rye arabinoxylan the preferred substrate on which the purified enzyme exhibited a K m of 10.6 ± 3.3 mg/ml and a V max of 29.2 ± 3.4 U/mg. Thin-layer chromatography analysis of hydrolysis products showed arabinose as the only sugar released by the enzyme from its substrates. The GH43 and CBM6 modules of the enzyme were individually cloned and expressed in Escherichia coli. While the isolated catalytic GH43 module did not show hydrolytic activity, the purified CBM6 bound to soluble arabinoxylan in affinity gel electrophoresis analysis. Evaluation of cooperative activity of arabinofuranosidase Abf43A with xylanases from families GH10, GH11, and GH30, (Xyn10A, Xyn11E, and Xyn30D from Paenibacillus barcinonensis) on arabinoxylan depolymerization revealed that the studied enzyme showed synergism with Xyn11E, a 2.54-fold increase in the amount of sugars released. On the contrary, Abf43A did not show synergism with the xylanases of families GH10 or GH30 evaluated. The enzyme characterized contributes to understanding the role of this class of enzymes in the catalytic depolymerization of arabinoxylans and their potential for the production of valuable xylooligosaccharides from these abundant plant polymers. Keywords Arabinoxylan Bioconversion Enzymatic hydrolysis GH43, synergism