Three-dimensional hydrodynamic flow focusing of dye, particles and cells in a microfluidic device by employing two bends of opposite curvature

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• 作者：Siddhartha Tripathi ; Amit Kumar ; Y. V. Bala Varun Kumar…
• 关键词：Three ; dimensional hydrodynamic focusing ; Secondary flow ; Microchannel ; Confocal microscopy ; Discrete particle model ; based simulation
• 刊名：Microfluidics and Nanofluidics
• 出版年：2016
• 出版时间：February 2016
• 年：2016
• 卷：20
• 期：2
• 全文大小：1,505 KB
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• 作者单位：Siddhartha Tripathi (1)
  Amit Kumar (1)
  Y. V. Bala Varun Kumar (1)
  Amit Agrawal (1)
  
  1. Department of Mechanical Engineering, Indian Institute of Technology Bombay, Powai, Mumbai, 400076, India
  
• 刊物类别：Engineering
• 刊物主题：Engineering Fluid Dynamics
  Medical Microbiology
  Polymer Sciences
  Nanotechnology
  Mechanics, Fluids and Thermodynamics
  Engineering Thermodynamics and Transport Phenomena
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1613-4990

文摘

This work relates to three-dimensional (3D) hydrodynamic flow focusing, wherein sample is encapsulated by sheath fluid in all the directions, making it a preferred method for particle focusing. Given the complex phenomenon involved in achieving 3D hydrodynamic focusing, we have been able to demonstrate a relatively simple microdevice for achieving this objective. In this work, a novel approach for 3D focusing utilizing two bends of opposite curvature in microchannel is proposed and demonstrated through experiments and numerical simulations. The proposed microdevice is fabricated on a single layer of polydimethylsiloxane and a single sheath inlet is used, thereby simplifying the 3D focusing mechanism and reducing the requirements of cost enhancing accessories. The mechanism underlying particle focusing is examined in detail. This microdevice provides several distinct advantages over other designs mentioned in the literature. Keywords Three-dimensional hydrodynamic focusing Secondary flow Microchannel Confocal microscopy Discrete particle model-based simulation