Tracking dynamics of enzyme activities and their gene expression in Picrorhiza kurroa with respect to picroside accumulation

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• 作者：Varun Kumar ; Kirti Shitiz…
• 关键词：Hexokinase ; Pyruvate kinase ; Isocitrate dehydrogenase ; Malate dehydrogenase ; Malic enzyme ; Secondary metabolism ; Picroside ; I
• 刊名：Journal of Plant Biochemistry and Biotechnology
• 出版年：2016
• 出版时间：April 2016
• 年：2016
• 卷：25
• 期：2
• 页码：125-132
• 全文大小：407 KB
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• 作者单位：Varun Kumar (1)
  Kirti Shitiz (1)
  Rajinder Singh Chauhan (1)
  Hemant Sood (1)
  Chanderdeep Tandon (2)
  
  1. Department of Biotechnology and Bioinformatics, Jaypee University of Information Technology, Waknaghat, 173234, Solan, HP, India
  2. Department of Biotechnology, Amity Institute of Biotechnology, Noida, 201313, India
  
• 刊物主题：Life Sciences, general; Plant Biochemistry; Protein Science; Receptors; Cell Biology;
• 出版者：Springer India
• ISSN：0974-1275

文摘

Picrosides, the terpenoids synthesized by Picrorhiza kurroa, have ample usage in medicine. Identification of the regulatory enzymes involved in picroside biosynthesis needs to be explored for improving the level of these secondary metabolites. Current efforts are based on the analysis of secondary metabolism in picroside biosynthesis but its interpretation is limited by the lack of information on the involvement of primary metabolic pathways. The present study investigated the connection of primary metabolic enzymes with the picrosides levels in P. kurroa. The results showed changes in the catalytic activities as well as in the gene expression profiles of hexokinase, pyruvate kinase, isocitrate dehydrogenase, malate dehydrogenase, and NADP+-malic enzyme in congruence with picroside-I content under different conditions of P. kurroa growth, which indicates the role of these enzymes in the accumulation of picrosides. The significant correlation coefficients (p < 0.05) observed between gene expression and enzyme activity underline the role of integrative studies for a better understanding of connecting links between metabolic pathways leading to picroside biosynthesis. This is apparently the first report on the involvement of glycolytic and TCA cycle enzymes in the accumulation of picrosides in P. kurroa.