Adipogenic potential of stem cells derived from rabbit subcutaneous and visceral adipose tissue in vitro
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- 作者：Ekaterina Vachkova ; D. Bosnakovski…
- 关键词：Rabbits ; Subcutaneous and visceral ADSC ; Supernatants ; Adipose differentiation potential
- 刊名：In Vitro Cellular & Developmental Biology - Animal
- 出版年：2016
- 出版时间：September 2016
- 年：2016
- 卷：52
- 期：8
- 页码：829-837
- 全文大小：2,645 KB
- 刊物主题：Cell Biology; Developmental Biology; Stem Cells; Cell Culture; Animal Genetics and Genomics;
- 出版者：Springer US
- ISSN：1543-706X
- 卷排序：52
文摘
Rabbits are considered as appropriate animal models to study some obesity-associated abnormalities because of the similarity of their blood lipid profile and metabolism to humans. The current study was focused on comparison of adipose differentiation ability in rabbit adipose-derived stem cells (ADSC) in vitro. Subcutaneous and visceral stromal vascular fractions (SVF) were isolated from three 28-d-old New Zealand rabbits by collagenase digestion. Supernatants from both isolates were collected 24 h after the initial plating. On the fourth passage, all isolated cell types undergo triplicate adipogenic induction. The adipose induction potential was calculated as percentage of increasing optical density (OD) values. The data revealed that with increasing the number of induction cycles, the induction tendency in visceral ADSC decreased in contrast to the subcutaneous ones. Although the supernatants did not reach induction levels of their relevant precursors, they follow the same pattern in both subcutaneous and visceral ADSC. All cell types successfully passed osteogenic and chondrogenic differentiation. In conclusion, the best adipose induction ability was observed in directly plated subcutaneous cell population. The increase of induction numbers depressed adipose induction ability in cell populations derived from visceral fat depots.