TNT Biotransformation and Detoxification by a Pseudomonas Aeruginosa Strain

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• 作者：Byung-Taek Oh ; Patrick J. Shea ; Rhae A. Drijber ; Galina K. Vasilyeva and Gautam Sarath
• 关键词：biotransformation ; detoxification ; pH ; Pseudomonas aeruginosa ; TNT
• 刊名：Biodegradation
• 出版年：2003
• 出版时间：October, 2003
• 年：2003
• 卷：14
• 期：5
• 页码：309-319
• 全文大小：6 KB
• 刊物类别：Earth and Environmental Science
• 刊物主题：Environment
  Soil Science and Conservation
  Geochemistry
  Terrestrial Pollution
  Waste Water Technology, Water Pollution Control, Water Management and Aquatic Pollution
  Waste Management and Waste Technology
  
• 出版者：Springer Netherlands
• ISSN：1572-9729

文摘

Successful microbial-mediated remediation requires transformationpathways that maximize metabolism and minimize the accumulation of toxic products. Pseudomonas aeruginosa strain MX, isolated from munitions-contaminated soil, degraded 100 mg TNT L-1 in culture medium within 10 h under aerobic conditions. The major TNT products were 2-amino-4,6-dinitrotoluene (2ADNT, primarily in the supernatant) and 2,2'-azoxytoluene (2,2'AZT, primarily in the cell fraction), which accumulated as major products via the intermediate2-hydroxylamino-4,6-dinitrotoluene (2HADNT). The 2HADNT and2,2'AZT were relatively less toxic to the strain than TNT and 2ADNT. Aminodinitrotoluene (ADNT) production increased when yeast extract was added to the medium. While TNT transformation rate was not affected by pH, more HADNTs accumulated at pH 5.0 than at pH 8.0 and AZTs did not accumulate at the lower pH. The appearance of 2,6-diamino-4-nitrotoluene (2,6DANT) and 2,4-diamino-6-nitrotoluene (2,4DANT); dinitrotoluene (DNT) and nitrotoluene (NT); and 3,5-dinitroaniline (3,5DNA) indicated various routes of TNT metabolism and detoxification by P. aeruginosa strain MX.