Mechanisms of Intracellular Calcium Homeostasis in MC3T3-E1 Cells and Bone Tissues of Sprague-Dawley Rats Exposed to Fluoride

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• 作者：Xiao-qin Duan ; Yan-hui Li ; Xiu-yun Zhang ; Zhi-tao Zhao…
• 关键词：Osteoblast (OB) ; Fluoride ; Intracellular calcium homeostasis ; Calcium channel ; Calcium pump
• 刊名：Biological Trace Element Research
• 出版年：2016
• 出版时间：April 2016
• 年：2016
• 卷：170
• 期：2
• 页码：331-339
• 全文大小：716 KB
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• 作者单位：Xiao-qin Duan (1)
  Yan-hui Li (1)
  Xiu-yun Zhang (2)
  Zhi-tao Zhao (2)
  Ying Wang (3)
  Huan Wang (1)
  Guang-sheng Li (2)
  Ling Jing (2)
  
  1. The Second Hospital Norman Bethune of Jilin University, Changchun, 130041, People’s Republic of China
  2. Institute of Endemic Disease of Jilin University, 1163 Xinmin Street, Changchun, 130021, People’s Republic of China
  3. The First Hospital Norman Bethune of Jilin University, Changchun, 130021, People’s Republic of China
  
• 刊物主题：Biochemistry, general; Biotechnology; Nutrition; Oncology;
• 出版者：Springer US
• ISSN：1559-0720

文摘

Calcium homeostasis of osteoblasts (OBs) has an important role in the physiology and pathology of bone tissue. In order to study the mechanisms of intracellular calcium homeostasis, MC3T3-E1 cells and Sprague-Dawley rats were treated with different concentrations of fluoride. Then, we examined intracellular-free calcium ion ([Ca2+]i) in MC3T3-E1 cells as well as mRNA and protein levels of Cav1.2, the main subunit of L-type voltage-dependent calcium channels (VDCCs), Na+/Ca2+ exchange carriers (NCS), and plasma membrane Ca2+-ATPase (PMCA), inositol 1,4,5-trisphosphate receptor (IP3R) channels, sarco/endoplasmic reticulum calcium ATPase 2b (SERCA2b)/ATP2A2 in vitro, and rat bone tissues in vivo. Our results showed that [Ca2+]i of fluoride-treated OBs increased in a concentration-dependent manner with an increase in the concentration of fluoride. We also found that the low dose of fluoride led to high expression levels of Cav1.2, NCS-1, and PMCA and low expression levels of IP3R and SERCA2b/ATP2A2, while the high dose of fluoride induced an increase in SERCA2b/ATP2A2 levels and decrease in Cav1.2, PMCA, NCS-1, and IP3R levels. These results demonstrate that calcium channels and calcium pumps of plasma and endoplasmic reticulum (ER) membranes keep intracellular calcium homeostasis by regulating Cav1.2, NCS-1, PMCA, IP3R, and SERCA2b/ATP2A2 expression.