Restriction of HIV-1-based lentiviral vectors in adult primary marrow-derived and peripheral mobilized human CD34+ hematopoietic stem and progenitor cells occurs prior to viral DNA integration
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- 作者：Daniel O. Griffin ; Stephen P. Goff
- 关键词：HIV ; Restriction ; Lentivirus ; Integration ; 2 ; LTR ; CD34+ ; Stem cells ; Progenitor cells
- 刊名：Retrovirology
- 出版年：2016
- 出版时间：December 2016
- 年：2016
- 卷：13
- 期：1
- 全文大小：1,833 KB
- 刊物主题：Virology; Infectious Diseases; Cancer Research;
- 出版者：BioMed Central
- ISSN：1742-4690
文摘
Background Gene therapy is currently being attempted using a number of delivery vehicles including lentiviral-based vectors. The delivery and insertion of a gene using lentiviral-based vectors involves multiple discrete steps, including reverse transcription of viral RNA into DNA, nuclear entry, integration of viral DNA into the host genome and expression of integrated genes. Transduction of murine stem cells by the murine leukemia viruses is inefficient because the expression of the integrated DNA is profoundly blocked. Transduction of human stem cells by lentivirus vectors is also inefficient, but the cause and specific part of the retroviral lifecycle where this block occurs is unknown.