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作者单位:Mayank Pokhriyal (1) O. P. Verma (2) Barkha Ratta (1) Ajay Kumar (1) Meeta Saxena (1) Bhaskar Sharma (1)
1. Division of Biochemistry, Indian Veterinary Research Institute, Izatnagar, Bareilly, U.P, 243122, India 2. Department of Molecular and Cellular Engineering, Sam Higginbottom Institute of Agriculture, Technology and Sciences, Allahabad, U.P, 211007, India
刊物类别:Biomedical and Life Sciences
刊物主题:Life Sciences Microbiology Biotechnology
出版者:Springer New York
ISSN:1432-0991
文摘
Immediate early (IE) genes are transcribed immediately after infection in BHV1 from two different immediate early transcription units. It is reported that the immediate early transcription unit I (IE TU1) of Bovine herpesvirus 1 (BHV1) transcribes two proteins BICP0 and BICP4 from a single promoter by alternative splicing but with identical 5′UTR. We found that the transcripts of BICP0 and BICP4 have different 5′UTRs. The bioinformatics analysis shows two similar spatially arranged TATA less promoter for the two transcripts. The bioinformatics analysis also showed a similar promoter for the IE TU2 which transcribes BICP22. The data strongly suggest that BICP0 and BICP4 are transcribed from two different promoters. The transcript produced by each promoter is spliced specifically as opposed to what has been reported earlier. The BICP0 and BICP4 also show different levels of expression. The expression level of BICP4 continuously declines after attaining a peak level at 1 h, while BICP0 shows biphasic expression supporting the earlier observation that it is expressed from two different promoters.