Comparative analysis of microRNA profiles between adult Ascaris lumbricoides and Ascaris suum

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• 作者：Chang-Chun Shao (1) (2)
  Min-Jun Xu (2)
  Samer Alasaad (3)
  Hui-Qun Song (2)
  Lifei Peng (4)
  Jian-Ping Tao (1)
  Xing-Quan Zhu (2)
  
  1. College of Veterinary Medicine ; Yangzhou University ; Yangzhou ; Jiangsu Province ; 225009 ; PR China
  2. State Key Laboratory of Veterinary Etiological Biology ; Key Laboratory of Veterinary Parasitology of Gansu Province ; Lanzhou Veterinary Research Institute ; Chinese Academy of Agricultural Sciences ; Lanzhou ; Gansu Province ; 730046 ; PR China
  3. Estaci贸n Biol贸gica de Do帽ana ; Consejo Superior de Investigaciones Cient铆ficas (CSIC) ; Avda. Am茅rico Vespucio s/n 41092 ; Sevilla ; Spain
  4. Department of Parasitology & Clinical Parasitology ; Guangdong Medical College ; Zhanjiang ; Guangdong Province ; 524023 ; PR China
  
• 关键词：MicroRNA (miRNA) ; Ascaris lumbricoides ; Ascaris suum ; Comparative analysis
• 刊名：BMC Veterinary Research
• 出版年：2014
• 出版时间：December 2014
• 年：2014
• 卷：10
• 期：1
• 全文大小：456 KB
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• 刊物主题：Veterinary Medicine; Zoology; Transgenics;
• 出版者：BioMed Central
• ISSN：1746-6148

文摘

Background The parasitic nematodes Ascaris lumbricoides and A. suum are of great public health and economic significance, and the two taxa were proposed to represent a single species. miRNAs are known with functions of gene regulations at post-transcriptional level. Results We herein compared the miRNA profiles of A. lumbricoides and A. suum female adults by Solexa deep sequencing combined with bioinformatics analysis and stem-loop real-time PCR. Using the A. suum genome as the reference genome, we obtained 171 and 494 miRNA candidates from A. lumbricoides and A. suum, respectively. Among which, 74 miRNAs were shared between the two taxa, 97 and 420 miRNAs were A. lumbricoides and A. suum specific. Target and function prediction revealed a significant set of targets which are related to ovarian message protein, vitellogenin and chondroitin proteoglycan of the two nematodes. Enrichment analysis revealed that the percentages of most predicted functions of the miRNA targets were similar, with some taxon specific or taxon enhanced functions, such as different target numbers, specific functions (NADH dehydrogenase and electron carrier functions), etc. Conclusions This study characterized comparatively the miRNAs of adult A. lumbricoides and A. suum, and the findings provide additional evidence that A. lumbricoides and A. suum represent a single species. Due to the fast evolution nature of miRNAs and the different parasitic living conditions of humans and pigs, the phenomenon above might indicate a fast evolution of miRNAs of Ascaris in humans and pigs.