Inhibitory effect of marine cyanobacterial extract on biofilm formation and virulence factor production of bacterial pathogens causing vibriosis in aquaculture

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• 作者：Sivasubramanian Santhakumari ; Arunachalam Kannappan…
• 关键词：Aquaculture ; Vibriosis ; Antibiotic resistance ; Quorum sensing ; Cyanobacteria ; Hexadecanoic acid
• 刊名：Journal of Applied Phycology
• 出版年：2016
• 出版时间：February 2016
• 年：2016
• 卷：28
• 期：1
• 页码：313-324
• 全文大小：1,208 KB
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• 作者单位：Sivasubramanian Santhakumari (1)
  Arunachalam Kannappan (1)
  Shunmugiah Karutha Pandian (1)
  Nooruddin Thajuddin (2) (3)
  Ramaswamy Babu Rajendran (4)
  Arumugam Veera Ravi (1)
  
  1. Department of Biotechnology, Science Campus, Alagappa University, Karaikudi, 630 004, Tamil Nadu, India
  2. Department of Microbiology, Bharathidasan University, Tiruchirappalli, 620 024, Tamil Nadu, India
  3. Department of Botany and Microbiology, College of Science, King Saud University, Riyadh, 11451, Kingdom of Saudi Arabia
  4. Department of Environmental Biotechnology, Bharathidasan University, Tiruchirappalli, 620 024, Tamil Nadu, India
  
• 刊物主题：Plant Sciences; Freshwater & Marine Ecology; Plant Physiology; Ecology;
• 出版者：Springer Netherlands
• ISSN：1573-5176

文摘

Emergence of extended antibiotic resistance among bacterial pathogens often leads to the failure of existing antibiotics to treat bacterial infections; therefore, there is an urgent need to look for novel alternative treatment measures. The aim of this study was to evaluate the anti-quorum sensing (QS) potential of Synechococcus sp., to prevent the onset of bacterial infections as an alternate to antibiotics. A total of 110 marine cyanobacterial strains were screened for their anti-QS activity against biomarker strain Chromobacterium violaceum (ATCC 12472) and aquatic bacterial pathogens Vibrio harveyi (MTCC 3438) and Vibrio vulnificus (MTCC 1145). Of the 110 strains tested, the extract of unicellular algae Synechococcus sp. (Q-25) exhibited the efficient reduction in the production of violacein pigment of C. violaceum to the level of 82 %, bioluminescence of V. harveyi to 91 % and protease in V. vulnificus to 63 %. In V. harveyi and V. vulnificus, it exhibited a significant reduction of 71 and 84 % in biofilm formation and 66 and 68 % in EPS production, respectively, without any antibacterial activity. Confocal laser scanning microscopic and light microscopic analyses further confirmed that the Q-25 extract effectively prevented initial attachment as well as disrupting the architecture of mature biofilm, when compared to their untreated controls. In addition, the characterization of active principle by gas chromatography–mass spectrometry analysis confirmed the presence of stable bioactive compound hexadecanoic acid in the extract. Hence, this study clearly revealed the antibiofilm and QS inhibitory potential of the cyanobacterium, Synechococcus sp. Keywords Aquaculture Vibriosis Antibiotic resistance Quorum sensing Cyanobacteria Hexadecanoic acid