Subchronic effects of cadmium on the gonads, expressions of steroid hormones and sex-related genes in tilapia Oreochromis niloticus

详细信息    查看全文

• 作者：Yongju Luo ; Dan Shan ; Huan Zhong ; Yi Zhou ; Wenzhi Chen ; Jinling Cao…
• 关键词：Nile tilapia ; Cadmium ; Reproduction ; Maternal transfer ; Gonad
• 刊名：Ecotoxicology
• 出版年：2015
• 出版时间：December 2015
• 年：2015
• 卷：24
• 期：10
• 页码：2213-2223
• 全文大小：1,473 KB
• 参考文献：Almeida J, Diniz Y, Marques S, Faine L, Ribas B, Burneiko R, Novelli E (2002) The use of the oxidative stress responses as biomarkers in Nile tilapia (Oreochromis niloticus) exposed to in vivo cadmium contamination. Environ Int 27:673-79CrossRef
  Beattie J, Pascoe D (1978) Cadmium uptake by rainbow trout, Salmo gairdneri eggs and alevins. J Fish Biol 13:631-37CrossRef
  Beyersmann D, Hechtenberg S (1997) Cadmium, gene regulation, and cellular signalling in mammalian cells. Toxicol Appl Pharmacol 144:247-61CrossRef
  Brown V, Shurben D, Miller W, Crane M (1994) Cadmium toxicity to rainbow trout Oncorhynchus mykiss Walbaum and brown trout Salmo trutta L. over extended exposure periods. Ecotoxicol Environ Saf 29:38-6CrossRef
  Bustin S (2002) Quantification of mRNA using real-time reverse transcription PCR (RT-PCR): trends and problems. J Mol Endocrinol 29:23-9CrossRef
  Cicik B, Ay ?, Karayakar F (2004) Effects of lead and cadmium interactions on the metal accumulation in tissue and organs of the Nile tilapia (Oreochromis niloticus). Bull Environ Contam Toxicol 72:141-48CrossRef
  Colt J (2006) Water quality requirements for reuse systems. Aquacult Eng 34:143-56CrossRef
  Costa V, Louren?o HM, Figueiredo I, Carvalho L, Lopes H, Farias I, Pires L, Afonso C, Vieira AR, Nunes ML (2009) Mercury, cadmium and lead in black scabbardfish (Aphanopus carbo Lowe, 1839) from mainland Portugal and the Azores and Madeira archipelagos. Sci Mar 73:77-8CrossRef
  Dang F, Wang WX (2009) Assessment of tissue-specific accumulation and effects of cadmium in a marine fish fed contaminated commercially produced diet. Aquat Toxicol 95:248-55CrossRef
  Dang Z, Lock RA, Flik G, Wendelaar Bonga SE (2000) Na+/K+-ATPase immunoreactivity in branchial chloride cells of Oreochromis mossambicus exposed to copper. J Exp Biol 203:379-87
  Das S, Mukherjee D (2013) Effect of cadmium chloride on secretion of 17β-estradiol by the ovarian follicles of common carp, Cyprinus carpio. Gen Comp Endocrinol 181:107-14CrossRef
  Flouriot G, Pakdel F, Valotaire Y (1996) Transcriptional and post-transcriptional regulation of rainbow trout estrogen receptor and vitellogenin gene expression. Mol Cell Endocrinol 124:173-83CrossRef
  Fostier A, Jalabert B, Billard R, Breton B, Zohar Y (1983) The gonadal steroids. Fish Physiol 9:277-72CrossRef
  Fuhr BJ, Rabenstein DL (1973) Nuclear magnetic resonance studies of the solution chemistry of metal complexes. IX. Binding of cadmium, zinc, lead, and mercury by glutathione. J Am Chem Soc 95:6944-950CrossRef
  Galvez F, Franklin NM, Tuttle RB, Wood CM (2007) Interactions of waterborne and dietary cadmium on the expression of calcium transporters in the gills of rainbow trout: influence of dietary calcium supplementation. Aquat Toxicol 84:208-14CrossRef
  Garcia-Santos S, Fontaínhas-Fernandes A, Wilson JM (2006) Cadmium tolerance in the Nile tilapia (Oreochromis niloticus) following acute exposure: assessment of some ionoregulatory parameters. Environ Toxicol 21:33-6CrossRef
  Garcia-Santos S, Monteiro S, Malakpour-Kolbadinezhad S, Fontaínhas-Fernandes A, Wilson J (2015) Effects of Cd injection on osmoregulation and stress indicators in freshwater Nile tilapia. Comp Biochem Physiol C 167:81-9
  Gill TS, Tewari H, Pande J (1991) In vivo and in vitro effects of cadmium on selected enzymes in different organs of the fish Barbus conchonius ham. (Rosy Barb). Comp Biochem Physiol C 100:501-05CrossRef
  Godt J, Scheidig F, Grosse-Siestrup C, Esche V, Brandenburg P, Reich A, Groneberg DA (2006) The toxicity of cadmium and resulting hazards for human health. J Occup Med Toxicol 1:1-CrossRef
  Gu DE, Mu XD, Luo D, Li YY, Yang YX, Xu M, Hu YC (2012) The study of population establishment of tilapia in main rivers in Guangdong Province. J Biosafety 21:277-82 (in Chinese)
  Hook SE, Fisher NS (2001) Sublethal effects of silver in zooplankton: importance of exposure pathways and implications for toxicity testing. Environ Toxicol Chem 20:568-74CrossRef
  Huang XD, Zhang JS, You ZL, Wang YQ, Liu LJ, Qi YL, Qiao TJ, An N (2007) Emergent treatment techniques and engineering measures for cadmium pollution in Guangdong Beijiang River. Water Technol 1:25-7
  Ijiri S, Kaneko H, Kobayashi T, Wang DS, Sakai F, Paul-Prasanth B, Nakamura M, Nagahama Y (2008) Sexual dimorphic expression of genes in gonads during early differentiation of a teleost fish, the Nile tilapia Oreochromis niloticus. Biol Reprod 78:333-41CrossRef
  Kumari M, Dutt NG (1991) Cadmium-induced histomorphological changes in the testis and pituitary gonadotrophic hormone secreting cells of the cyprinid Puntius sarana. Ital J Zool 58:71-6
  Lacoue-Labarthe T, Warnau M, Oberh?nsli F, Teyssié JL, Koueta N, Bustamante P (2008) Differential bioaccumulation behaviour of Ag and Cd during the early development of the cuttlefish Sepia officinalis. Aquat Toxicol 86:437-46CrossRef
  Le Guevel R, Petit F, Le Goff P, Métiv
• 作者单位：Yongju Luo (1) (2) (3)
  Dan Shan (3)
  Huan Zhong (2)
  Yi Zhou (2)
  Wenzhi Chen (3)
  Jinling Cao (4)
  Zhongbao Guo (2)
  Jun Xiao (2)
  Fulin He (3)
  Yifan Huang (5)
  Jian Li (5)
  Heming Huang (5)
  Pao Xu (1) (6)
  
  1. Wuxi Fisheries College, Nanjing Agricultural University, Wuxi, 214081, Jiangsu, China
  2. Guangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture, Guangxi Academy of Fishery Sciences, Nanning, 530021, Guangxi, China
  3. Faculty of Fisheries and Life Science, Shanghai Ocean University, Shanghai, 201306, China
  4. State Key Laboratory of Ecological Animal Husbandry and Environmental Veterinary Medicine, College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu, 030801, Shanxi, China
  5. Guangxi Autonomous Region Center for Analysis and Test Research, Nanning, 530022, Guangxi, China
  6. Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Scientific Observing and Experimental Station of Fishery Resources and Environment in the Lower Reaches of the Yangtze River, Wuxi, 214081, Jiangsu, China
  
• 刊物类别：Earth and Environmental Science
• 刊物主题：Environment
  Environment
  Monitoring, Environmental Analysis and Environmental Ecotoxicology
  Ecology
  Environmental Management
  
• 出版者：Springer Netherlands
• ISSN：1573-3017

文摘

Cadmium (Cd) is one of the most toxic heavy metals in aquatic ecosystem which affects fish health and aquaculture. In the present study, we examined the bioaccumulation of Cd in the gonads of tilapia via dissolved and dietary routes. We evaluated the subchronic effects of Cd on the histology of gonads, steroid hormone levels and sex-related gene expressions in tilapia. In addition, we also studied maternal transfer of Cd. Our results indicated that Cd was accumulated significantly in both ovary and testis from both exposure routes. Histopathological analysis showed that Cd induced ovary and testis injuries. Estradiol levels were significantly increased in dissolved Cd exposed female fish. In addition, the Cd exposure displayed different effects on gene expressions in gonads. In females, the estrogen receptor (ERα) was stimulated in dissolved Cd-exposed fish at 70.32 and 143.78 μg/L for 30 days and in fish at 143.78 μg/L for 60 days. Vitellogenin expression was significantly down-regulated in the ovary of dissolved Cd-exposed fish. In testis, GR expression was elevated after 60 days of dissolved Cd and dietary exposure. Furthermore, Cd level was significantly higher in the eggs than that in the fry. Our results demonstrated that both dissolved and dietary Cd exposures affected gonad development by altering steroid hormone levels and sex-related gene expressions in tilapia. Keywords Nile tilapia Cadmium Reproduction Maternal transfer Gonad