In situ study of the state of lysozyme molecules at the very early stage of the crystallization process by small-angle X-ray scattering

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• 作者：M. A. Marchenkova ; V. V. Volkov ; A. E. Blagov ; Yu. A. Dyakova…
• 刊名：Crystallography Reports
• 出版年：2016
• 出版时间：January 2016
• 年：2016
• 卷：61
• 期：1
• 页码：5-10
• 全文大小：722 KB
• 参考文献：1.E. R. Abraham and R. Robinson, Nature 140 (3531), 24 (1937).CrossRef ADS
  2.M. M. Ries-Kautt and A. F. Ducruix, J. Biol. Chem. 264 (2), 745 (1989).
  3.Z. Kam, H. B. Shore, and G. Feher, J. Mol. Biol. 123 (4), 539 (1978).CrossRef
  4.M. L. Pusey, R. S. Snyder, and R. Naumann, J. Biol. Chem. 261 (14), 6524 (1986).
  5.G. D. Ilyushin and Yu. V. Pisarevskii, Crystallogr. Rep. 60 (6), 776 (2015).CrossRef ADS
  6.M. V. Koval’chuk, P. A. Prosekov, M. A. Marchenkova, et al., Crystallogr. Rep. 59 (5), 679 (2014).CrossRef ADS
  7.M. Wiechmann, O. Enders, S. Zeilinger, et al., Ultramicroscopy 86 (1–2), 159 (2001).CrossRef
  8.I. V. Yaminsky, N. V. Gvozdev, M. I. Sil’nikova, et al., Crystallogr. Rep. 47 (1), 149 (2002).CrossRef ADS
  9.S. D. Durbin and G. Feher, J. Mol. Biol. 212 (4), 763 (1990).CrossRef
  10.A. Nadarajah, M. Li, and M. L. Pusey, Acta Crystallogr. D 53, 524 (1997).CrossRef
  11.M. Li, A. Nadarajah, and M. L. Pusey, Acta Crystallogr. D 55, 1012 (1999).CrossRef
  12.C. Hai Liang, Y. Yu, W.-Ch. Chen, et al., Chin. Chem. Lett. 17 (1), 101 (2006).
  13.F. Boué, F. Lefaucheux, M. C. Robert, et al., J. Cryst. Growth 133 (3–4), 246 (1993).CrossRef ADS
  14.D. I. Svergun and L. A. Feigin, X-Ray and Small-Angle Neutron Scattering (Nauka, Moscow, 1986) [in Russian].
  15.L. Yu. Mogilevskii, A. T. Dembo, D. I. Svergun, et al., Kristallografiya 29 (3), 587 (1984).
  16.P. Emsley, B. Lohkamp, W. G. Scott, et al., Acta Crystallogr. D 66 (4), 486 (2010).CrossRef
  17.http://pymol.org/.
  18.P. V. Konarev, M. V. Petoukhov, V. V. Volkov, et al., J. Appl. Crystallogr. 39 (2), 277 (2006).CrossRef
  19. GOST (State Standard) 8.698–2010: State System for Ensuring Uniform Measurements. Size Parameters of Nanoparticles and Thin Films. Technique of Measurements with a Small-Angle X-Ray Diffractometer (Standartinform, Moscow, 2010) [in Russian].
  20.P. V. Konarev, V. V. Volkov, A. V. Sokolova, et al., J. Appl. Crystallogr. 36 (5), 1277 (2003).CrossRef
  21.http://www.embl-hamburg.de/biosaxs/oligomer.html.
  22.D. Svergun, C. Barberato, and M. H. Koch, J. Appl. Crystallogr. 28 (6), 768 (1995).CrossRef
  
• 作者单位：M. A. Marchenkova (1) (2)
  V. V. Volkov (1) (2)
  A. E. Blagov (1) (2)
  Yu. A. Dyakova (1) (2)
  K. B. Ilina (1) (2)
  E. Yu. Tereschenko (1) (2)
  V. I. Timofeev (1) (2)
  Yu. V. Pisarevsky (1) (2)
  M. V. Kovalchuk (1) (2)
  
  1. Shubnikov Institute of Crystallography, Russian Academy of Sciences, Leninskii pr. 59, Moscow, 119333, Russia
  2. National Research Centre “Kurchatov Institute,”, pl. Akademika Kurchatova 1, Moscow, 123098, Russia
  
• 刊物类别：Physics and Astronomy
• 刊物主题：Physics
  Crystallography
  Russian Library of Science
  
• 出版者：MAIK Nauka/Interperiodica distributed exclusively by Springer Science+Business Media LLC.
• ISSN：1562-689X

文摘

The molecular state of hen egg white lysozyme in solution has been studied by small-angle X-ray scattering (SAXS) combined with molecular simulation. The addition of a precipitant is shown to change the state of the protein molecules in solution. The SAXS data were processed using the constructed models of different oligomers. Under the crystallization conditions, lysozyme is shown to be present in solution as monomers (96.0%), dimers (1.9%), and octamers (2.1%), whereas tetramers and hexamers are not found. The modeled structure of the octamer is not consistent with the commonly accepted unit cell containing eight lysozyme molecules. Meanwhile, the modeled octamers are well-fitted to the crystal structure and can serve as building blocks in the course of crystal growth. Original Russian Text © M.A. Marchenkova, V.V. Volkov, A.E. Blagov, Yu.A. Dyakova, K.B. Ilina, E.Yu. Tereschenko, V.I. Timofeev, Yu.V. Pisarevsky, M.V. Kovalchuk, 2016, published in Kristallografiya, 2016, Vol. 61, No. 1, pp. 10–15.