Hydrogen peroxide causes Vibrio vulnificus bacteriolysis accelerated by sulfonyl fluoride compounds

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• 作者：Michinobu Yoshimura ; Tetsuro Tamura ; Ken-ichiro Iida…
• 关键词：Vibrio vulnificus ; Bacteriolysis ; Hydrogen peroxide ; Hydroxyl radical ; Sulfonyl fluoride compounds ; Fenton reaction
• 刊名：Archives of Microbiology
• 出版年：2015
• 出版时间：November 2015
• 年：2015
• 卷：197
• 期：9
• 页码：1075-1085
• 全文大小：701 KB
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• 作者单位：Michinobu Yoshimura (1) (3)
  Tetsuro Tamura (1)
  Ken-ichiro Iida (1)
  Susumu Shiota (2)
  Hiroaki Nakayama (1)
  Shin-ichi Yoshida (1)
  
  1. Department of Bacteriology, Graduate School of Medical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka, 812-8582, Japan
  3. Division of Medical Oncology, Hematology and Infectious Disease, Department of Internal Medicine, Fukuoka University, 7-45-1 Nanakuma, Jonan, Fukuoka, 814-0180, Japan
  2. Department of Oral Health, Growth and Development, Division of Oral Infectious Diseases and Immunology,Graduate School of Dental Science, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka, 812-8582, Japan
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Microbiology
  Microbial Ecology
  Biochemistry
  Cell Biology
  Biotechnology
  Ecology
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1432-072X

文摘

Induction of bacteriolysis of Vibrio vulnificus cells by 10 mM hydrogen peroxide (H₂O₂) was analyzed. All Vibrio species examined, except for Vibrio hollisae, were lysed by 10 mM H₂O₂. Bacteriophage induction was not the cause of H₂O₂-induced bacteriolysis. Autolysis is also known to cause bacteriolysis. VvpS protein is a serine protease of V. vulnificus essential for autolysis. vvpS mutant underwent H₂O₂-induced bacteriolysis in the same manner as the wild type. Protease inhibitors including serine protease inhibitors did not inhibit H₂O₂-induced bacteriolysis, which means that bacteriolysis is not due to autolysis. Unexpectedly, H₂O₂-induced bacteriolysis was accelerated by adding 4-(2-aminoethyl) benzenesulfonyl fluoride hydrochloride (AEBSF) and phenylmethylsulfonyl fluoride which are serine protease inhibitors. The hydroxyl radical was generated by H₂O₂–AEBSF interaction. It was considered that H₂O₂-induced bacteriolysis was caused by the hydroxyl radical which was generated by Fenton reaction, and possibly mediated by AEBSF. Deferoxamine, an agent chelating ferric ion and Fenton reaction inhibitor, suppressed both H₂O₂-induced bacteriolysis and its acceleration by AEBSF. This suggests that both phenomena were Fenton reaction dependent, and hydroxyl radical generated by Fenton reaction caused bacteriolysis of V. vulnificus though the reason for high susceptibility of Vibrio species to hydroxyl radical is not known. Keywords Vibrio vulnificus Bacteriolysis Hydrogen peroxide Hydroxyl radical Sulfonyl fluoride compounds Fenton reaction