Characterization of antibiotic resistant and enzyme producing bacterial strains isolated from the Arabian Sea
详细信息 查看全文
- 作者：Preeti N. Tallur ; Dayanand B. Sajjan ; Sikandar I. Mulla ; Manjunatha P. Talwar…
- 关键词：Extracellular enzymes ; Lysinibacillus sphaericus ; Vibrio vulnificus ; Bacillus toyonensis ; Antibiotics ; Aromatic compounds
- 刊名：3 Biotech
- 出版年：2016
- 出版时间：December 2016
- 年：2016
- 卷：6
- 期：1
- 全文大小：1,638 KB
- 参考文献：Aisha N, Nuzhat A (2011) Isolation and characterization of indigenous luminescent marine bacteria from Karachi coast. Acad Res Int 2(2):74–83
Ashadevi NK, Balakrishnan K, Gopal R, Padmavathy S (2008) Bacillus clausii MB9 from the east coast regions of India: isolation, biochemical characterization and antimicrobial potentials. Curr Sci 95(5):627–636
Aureen G, Ramesh R, Saroj B (2010) Bacteria from sand dunes of Goa promoting growth in egg plant. World J Agric Sci 6(5):555–564
Bal S, Mishra RR, Rath B, Sahu HK, Thatoi HN (2009) Isolation and extracellular enzyme activity of predominant marine Bacillus sp. isolated from sea water of Orissa coast. Malays J Microbiol 5(2):87–93
Bozal N, Montes MJ, Tudela E, Jimenez F, Guinea J (2002) Shewanella frigidimarina and Shewanella livingstonensis sp. nov. isolated from Antarctic coastal areas. Int J Syst Evol Microbiol 52:195–205CrossRef
Bradford MM (1976) Rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254CrossRef
Chiaki I, Usio S, Nobuo T (1985) Isolation and characterization of marine bacteria producing alkaline protease inhibitor. Bull Jpn Soc Sci Fish 52(5):799–803
Chin AWTF, Bloemberg GV, Mulders IH, Dekkers LC, Lugtenberg BJ (2000) Root colonization by phenazine-1-carboxamide-producing bacterium Pseudomonas chlororaphis PCL1391 is essential for biocontrol of tomato foot and root rot. Mol Plant Microbe Interact MPMI 13(12):1340–1345CrossRef
Coon HJ, Jenison MW, Weeks OB (1957) Manual of microbiological methods. Mc Graw Hill Book Co., Inc., New York
Couri S, Farias AX (1995) Genetic manipulation of Aspergillus niger for increased synthesis of pectinolytic enzymes. Rev Microbiol 26(4):314–317
Glina VV, Khatiel KM (2011) Cellulase enzyme activity of Aspergillus fumigates from Mangrove soil on lignocellulosic substrate. Recent Res Sci Technol 3(1):132–134
Greif D, Wesner D, Regtmeier J, Anselmetti D (2010) High resolution imaging of surface patterns of single bacterial cells. Ultramicroscopy 110(10):1290–1296CrossRef
Hackman RH (1962) Studies on chitin V. The action of mineral acids on chitin. Aust J Biol Sci 15:526–537
Holding AJ, Collee JG (1971) Routine biochemical tests. Academic Press, London and New York
Holt JG, Krieg NR, Sneath PHA, Staley JT, Williams ST (1994) Bergey’s manual of determinative bacteriology. Williams and Wilkins, Baltimore
Hoskeri RS, Mulla SI, Shouche YS, Ninnekar HZ (2011) Biodegradation of 4-chlorobenzoic acid by Pseudomonas aeruginosa PA01 NC. Biodegradation 22(3):509–516CrossRef
Hugh R, Leifson E (1953) The taxonomic significance of fermentative versus oxidative metabolism of carbohydrates by various gram-negative bacteria. J Bacteriol 66(1):24–26
Janani L, Gaurav Kumar KV, Rao B (2011) Screening of pectinase producing microorganisms from agricultural waste dump soil. Asian J Biochem Pharm Res 1(2):329–337
Jiang S, Lee JH, Kim MG, Myung NV, Fredrickson JK, Sadowsky MJ, Hur HG (2009) Biogenic formation of As-S nanotubes by diverse Shewanella strains. Appl Environ Microbiol 75(21):6896–6899CrossRef
Jiménez G, Blanch AR, Tamames J, Rosselló-Mora R (2013) Complete genome sequence of Bacillus toyonensis BCT-7112T, the active ingredient of the feed additive preparation toyocerin. Genome Announc 1(6):e01080–13CrossRef
Kiruthika J, Saraswathy N (2013) Production of l -glutaminase and its optimization from a novel marine isolate Vibrio azureus JK-79. Afr J Biotechnol 12(50):6944–6953
Krishnakumar S, Alexis Rajan R, Ravikumar S (2011) Extracellular production of L-glutaminase by marine alkalophilic Streptomyces sp. SBV1 isolated from Cape Comorin coast. Indian J Geo-mar Sci 40(5):717–721
Kuznetsov YG, McPherson A (2011) Atomic force microscopy in imaging of viruses and virus-infected cells. Microbiol Mol Biol Rev MMBR 75(2):268–285CrossRef
Ling S, Jeremy BF (2014) Uranium reduction by Shewanella oneidensis MR-1 as a function of NaHCO3 control of reduction kinects. Environ Sci Technol 48(7):3768–3775CrossRef
Lozano LC, Jenny D (2013) Metal tolerance and larvicidal activity of Lysinibacillus sphaericus. World J Microbiol Biotechnol 29:1383–1389CrossRef
Muhammad I, Asma S, Quratulain S, Muhammad N (2012) Isolation and screening of cellulolytic bacteria from soil and optimization of cellulase production and activity. Turk J Biochem 37(3):287–293CrossRef
Mulla SI, Talwar MP, Hoskeri RS, Ninnekar HZ (2012) Enhanced degradation of 3-nitrobenzoate by immobilized cells of Bacillus flexus strain XJU-4. Biotechnol Bioproc E 17(6):1294–1299CrossRef
Nazia J, Nuzhat A (2006) Characterization of bacterial flora from coastal areas of Pakistan. J Basic Appl Sci 2(2):89–93
Pankaj V, Prashant Kumar P, Arvind Kumar G, Ho Jun K, Keun SK, Chi NS, Milind SP, Yogesh SS (2011) Shewanella indica sp. nov., isolated from sediment of the Arabian Sea. Int J Syst Evolut Microbiol 61:2058–2064
Pankaj V, Chi NS, Prashant Kumar P, Ramesh RB, Cathrin S, Manfred R, Yogesh SS (2013) Bhargavaea indica sp. nov., a member of the phylum Firmicutes isolated from Arabian Sea sediments. J Microbiol 51(1):36–42
Rheinheimer G (1980) Aquatic microbiology. New York, John Wiley
Saima MD, Roohi IZ Ahmad (2013) Isolation of novel chitinolytic bacteria and production optimization of extracellular chitinase. J Genetic Eng Biotechnol 1:39–46CrossRef
Sanjay Kumar S, Nagappa R (2011) Denaturing gradient gel electrophoresis profiling of bacteria communities composition in Arabian Sea. J Environ Biol 32:339–346
Sambrook J, Fritsch EF, Maniatis T (1989) Molecular Cloning: A laboratory manual, vol 3, 2nd edn. Cold Springs Harbor Laboratory, Cold Springs Harbor, New York
Sebdani RM, Ardakani MR, Ghezelbash GR, Sadrinasab M (2011) Phylogenic characterisation of lipase producing Bacillus strains isolated from Persian Gulf sediments. Aust J Basic Appl Sci 5(4):121–126
Seeley HW, Van Dan Demark PJ (1972) Microbes in action-a laboratory manual of Microbiology. Freeman, San Francisco, p 361
Shreedevi SK, Rathod JL (2011) Isolation and enumeration of flora in false trevally, Lactarius lactarius of Karwar, central west coast of India. Indian J Geo-Mar Sci 40(4):583–586
Silva-Filha MHNL, Berry C, Regis L (2014) Chapter three—Lysinibacillus sphaericus: toxins and mode of action, applications for mosquito control and resistance management. Elsevier, Amsterdam
Sirisha E, Rajasekar N, Lakshmi Narasu M (2010) Isolation and optimization of lipase producing bacteria from oil contaminated soils. Adv Biol Res 4(5):249–252
Smibert RM, Krieg NR (1981) Manual of methods for general bacteriology. American Society for Microbiology, Washington
Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28(10):2731–2739CrossRef
Wiatrowski HA, Ward PM, Barkay T (2006) Novel reduction of mercury (II) by mercury-sensitive dissimilatory metal-reducing bacteria. Environ Sci Technol 40(21):6690–6696
Wirth MC, Berry C, Walton WE, Federici BA (2014) Mtx toxins from Lysinibacillus sphaericus enhance mosquitocidal cry-toxin activity and suppress cry-resistance in Culex quinquefasciatus. J Invertebr Pathol 115:62–67CrossRef
Yilmaz M, Soran H, Beyatli Y (2006) Antimicrobial activities of some Bacillus spp. strains isolated from the soil. Microbiol Res 161(2):127–131CrossRef
Yujun G, Rongying Z, Xiujuan Y, Shanshan G, Xiangqian L (2015) Highly efficient in vitro biosynthesis of silver nanoparticles using Lysinibacillus sphaericus MR-1 and their characterization. Sci Technol Adv Mater 16:015004. doi:10.1088/1468-6996/16/1/015004 CrossRef
Yulianti T, Chasanah E, Tambunan USF (2012) Screening and characterization of l -glutaminase produced by bacteria isolated from Sangihe Talaud Sea. Squalen 7(3):115–121
Zamudio-Maya M, Narvaez-Zapata J, Rojas-Herrera R (2008) Isolation and identification of lactic acid bacteria from sediments of a coastal marsh using a differential selective medium. Lett Appl Microbiol 46(3):402–407CrossRef - 作者单位：Preeti N. Tallur (1)
Dayanand B. Sajjan (2)
Sikandar I. Mulla (2)
Manjunatha P. Talwar (2)
A. Pragasam (1)
Vinayak M. Nayak (1)
Harichandra Z. Ninnekar (2)
Shivanand S. Bhat (1)
1. Government Arts and Science College, Karwar, Karnataka, India
2. Department of Biochemistry, Karnatak University, Dharwad, Karnataka, India - 刊物主题：Biotechnology; Agriculture; Cancer Research; Bioinformatics; Stem Cells; Biomaterials;
- 出版者：Springer Berlin Heidelberg
- ISSN：2190-5738
文摘
Marine bacteria are known to produce many bioactive molecules and extracellular enzymes of commercial importance. We have investigated the bacterial diversity of the coastal area of Karwar, Karnataka State, India. Among these bacterial isolates, five bacterial strains were selected and identified by their morphological, biochemical characteristics and phylogenetic analysis based on 16S rRNA gene sequences. The identified bacterial isolates, Bacillus toyonensis PNTB1, Lysinibacillus sphaericus PTB, Vibrio vulnificus PMD, Shewanella MPTDBS, and Pseudomonas chlororaphis PNTB were characterized for their tolerance to salt and antibiotics. Vibrio vulnificus PMD showed maximum tolerance at higher concentration of salt than other bacteria. These bacterial strains were screened for the production of extracellular enzymes such as lipase, cellulase, pectinase, tannase, chitinase, and l-glutaminase. Vibrio vulnificus showed maximum production of l-glutaminase enzyme. Bacillus toyonensis PNTB1 shows lipase, CM-cellulase and chitinase activities. These isolated bacterial cultures were also utilized most of the aromatic compounds at 7 mM. These findings indicate the organisms present in this zone may have more potential applications in bioremediation, agricultural, industrial, and therapeutics. Keywords Extracellular enzymes Lysinibacillus sphaericus Vibrio vulnificus Bacillus toyonensis Antibiotics Aromatic compounds