A novel mutation in TFL1 homolog affecting determinacy in cowpea (Vigna unguiculata)
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- 作者：P. Dhanasekar ; K. S. Reddy
- 关键词：Determinate growth habit ; Terminal flower ; Point mutation ; VuTFL1 ; Cowpea ; Vigna unguiculata
- 刊名：Molecular Genetics and Genomics
- 出版年：2015
- 出版时间：February 2015
- 年：2015
- 卷：290
- 期：1
- 页码：55-65
- 全文大小：1,694 KB
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K. S. Reddy (1)
1. Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Centre, Trombay, Mumbai, 400 085, India - 刊物类别：Biomedical and Life Sciences
- 刊物主题：Life Sciences
Cell Biology
Biochemistry
Microbial Genetics and Genomics - 出版者：Springer Berlin / Heidelberg
- ISSN：1617-4623
文摘
Mutations in the widely conserved Arabidopsis Terminal Flower 1 (TFL1) gene and its homologs have been demonstrated to result in determinacy across genera, the knowledge of which is lacking in cowpea. Understanding the molecular events leading to determinacy of apical meristems could hasten development of cowpea varieties with suitable ideotypes. Isolation and characterization of a novel mutation in cowpea TFL1 homolog (VuTFL1) affecting determinacy is reported here for the first time. Cowpea TFL1 homolog was amplified using primers designed based on conserved sequences in related genera and sequence variation was analysed in three gamma ray-induced determinate mutants, their indeterminate parent “EC394763-and two indeterminate varieties. The analyses of sequence variation exposed a novel SNP distinguishing the determinate mutants from the indeterminate types. The non-synonymous point mutation in exon 4 at position 1,176 resulted from transversion of cytosine (C) to adenine (A) leading to an amino acid change (Pro-136 to His) in determinate mutants. The effect of the mutation on protein function and stability was predicted to be detrimental using different bioinformatics/computational tools. The functionally significant novel substitution mutation is hypothesized to affect determinacy in the cowpea mutants. Development of suitable regeneration protocols in this hitherto recalcitrant crop and subsequent complementation assay in mutants or over-expressing assay in parents could decisively conclude the role of the SNP in regulating determinacy in these cowpea mutants.