Riboflavin and chlorophyll as photosensitizers in electroformed giant unilamellar vesicles as food models

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• 作者：Hui-Jing Wang ; Ran Liang ; Hui-Hui Du ; Jing-Xuan Ai…
• 关键词：Giant unilamellar vesicle ; Lipid bilayer integrity ; β ; carotene ; Puerarin ; Antioxidant synergism
• 刊名：European Food Research and Technology
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：243
• 期：1
• 页码：21-26
• 全文大小：
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Food Science; Analytical Chemistry; Biotechnology; Agriculture; Forestry;
• 出版者：Springer Berlin Heidelberg
• ISSN：1438-2385
• 卷排序：243

文摘

Electroformed giant unilamellar vesicles (GUVs) were found to have optimal sizes (~10 µm average diameter) for studying effects of photosensitizers and antioxidants in lipid bilayers as food models. By using optical microscopy and digital image processing techniques, no membrane damage was found for hydrophilic riboflavin, while lipophilic chlorophyll a initiated GUV budding and subsequent disintegration under light irradiation, indicating that lipophilic photosensitizers are the more important in such structured lipids. Lipophilic β-carotene provided protection against oxidative damage induced by chlorophyll a as shown by an increased lag phase for budding; however, it had no effect on subsequent budding rate. Hydrophilic puerarin alone exhibited little protection in terms of lag phase, but decreased together with β-carotene budding rate after the lag phase by a factor of more than 2, showing a clear synergistic effect between a hydrophilic antioxidant, inactive in the aqueous phase alone, and a lipophilic antioxidant protecting lipids.