A New Thermostable and Organic Solvent-Tolerant Lipase from Staphylococcus warneri; Optimization of Media and Production Conditions Using Statistical Methods

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• 作者：Viraj U. Yele ; Krutika Desai
• 关键词：Solvent ; tolerant thermostable lipase ; Optimization ; Statistical approach ; PBD ; CCD ; RSM
• 刊名：Applied Biochemistry and Biotechnology
• 出版年：2015
• 出版时间：January 2015
• 年：2015
• 卷：175
• 期：2
• 页码：855-869
• 全文大小：1,877 KB
• 参考文献：1. Classification and nomenclature of enzymes by the reactions they catalyse. Enzyme Nomenclature1992. Available from http://www.chem.qmul.ac.uk/iubmb/enzyme/rules.html. Accessed 17 February, 2014
  2. Macrae, A. R. (1983). / Journal of American Oil Chemistry Society, 60(2), 291-94. CrossRef
  3. Pennisi, E. (1997). / Science, 276, 705-06. CrossRef
  4. Sellek, G. A., & Chaudhuri, J. B. (1999). / Enzyme Microbiology Technology, 25, 471-82. CrossRef
  5. Koops, B. C., Verheij, H. M., Slotboom, A. J., & Egmond, M. R. (1999). / Enzyme Microbiology Technology, 25, 622-31. CrossRef
  6. Helen, T., de Débora, O., Marcio, A. M., Marco, D. L., & Oliveira, J. V. (2010). / Food Bioprocess Technology, 3(2), 182-96. CrossRef
  7. Lima, C. J. B., Coelho, L. F., & Contiero, J. (2010). / Food Technology and Biotechnology, 48, 175-81.
  8. Kiruthika, P., Nisshanthini, S. D., Saraswathi, A., & Rajendiran, R. (2011). / International Journal Advances Biotechnology Research, 2, 422-30.
  9. Salihu, A., Bala, M., & Bala, S. M. (2013). / ISRN Biotechnology, 2013, 1-. doi:10.5402/2013/718352 . CrossRef
  10. Cheng, S. W., Wang, Y. F., & Liua, F. F. (2011). / Chemistry Biochemistry Engineering, 25, 377-83.
  11. Plackett, R. L., & Burman, J. P. (1946). / Biometrika, 33(4), 305-25. CrossRef
  12. Salihu, A., Alam, Z., Abdulkarim, M. I., & Salleh, M. (2011). / African Journal of Biotechnology, 10, 18704-8708. doi:10.5897/AJB11.2741 .
  13. Rajendiran, R., Gayathri, S., Sureshkumar, B. T., & Priya, V. A. (2011). / International Journal Agriculture Biology, 1, 60-3.
  14. Lo, C.-F., Yu, C.-Y., Kuan, I.-C., & Lee, S.-L. (2012). / International Journal of Molecular Sciences, 13, 14889-7. doi:10.3390/ijms131114889 . CrossRef
  15. Salihu, A., Alam, Z., Abdulkarim, M. I., & Salleh, M. (2011). / Journal of Molecular Catalysis B: Enzymatic, 69(1), 66-3. CrossRef
  16. Kaushik, R., Marwah, R. G., Gupta, P., et al. (2010). / Indian Journal of Microbiology, 50, 456-2. doi:10.1007/s12088-011-0100-y . CrossRef
  17. Talon, R., Dublet, N., Montel, M. C., et al. (1995). / Current Microbiology, 30(1), 11-6. CrossRef
  18. Kampen, M. D. V., Rosenstein, R., Gotz, F., et al. (2001). / Biochimica et Biophysica Acta, 1544, 229-41. CrossRef
  19. De Abreu, L., Lafuente, R. F., Rodrigues, R. C., et al. (2014). / Journal of Molecular Catalysis B: Enzymatic, 99, 51-5. CrossRef
  20. Ogino, H., Miyamoto, K., & Ishikawa, H. (1994). / Applied and Environmental Microbiology, 60(10), 3884-886.
  21. Sirisha, E., Rajasekar, N., & Narasu, M. L. (2010). / Advances Biology Research, 4, 249-52.
  22. Stathopoulou, P. M., Savvides, A. L., Karagouni, A. D., & Hatzinikolaou, D. G. (2013). / Biomedical Research International, 2013, 703130. doi:10.1155/2013/703130 . CrossRef
  23. Abdel-Fattah, Y. R., Soliman, N. A., Gaballa, A. A., Sabry, S. A., & El-Diwany, A. I. (2002). / Acta Microbiologica Polonica, 51(4), 353-6.
  24. Kishan, G., Gopalakannan
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Biotechnology
  Biochemistry
  
• 出版者：Humana Press Inc.
• ISSN：1559-0291

文摘

A new thermostable and solvent-tolerant lipase was isolated from newly isolated Staphylococcus warneri from oil-contaminated soil. Optimization of the fermentation media for production of thermostable and organic solvent-tolerant lipase was carried out using two statistical methods, i.e., Plackett–Burman design (PBD) and central composite design (CCD) were used for the optimization of the media components. PBD was used to efficiently select important medium components affecting the lipase production. Out of 15 medium components screened, four components, i.e., olive oil, peptone, maltose, and K2HPO4 were found to contribute positively to lipase production. CCD and response surface methodology (RSM) were used to determine the optimum levels of the selected components using Design-Expert 8.0 software. Production medium with olive oil (1.45?%), peptone (0.28?%), maltose (0.054?%), and K2HPO4 (0.091?%) was optimized with a maximum lipase production of 10.43?IU/ml/min. Similarly, production conditions for the lipase production were optimized by using CCD and RSM. Optimized conditions were found to have an incubation temperature of 55?°C, medium pH of 8.0, agitation of 120?rpm, and inoculum volume of 2?%. RSM revealed the maximum lipase production of 17.21?IU/ml using these optimized production conditions. Crude lipase showed enhanced activity in organic solvents such as diethyl ether, hexane, and cyclohexane.