Oxidized alpha-1 antitrypsin as a predictive risk marker of opisthorchiasis-associated cholangiocarcinoma

详细信息    查看全文

• 作者：Wassana Jamnongkan (1) (2)
  Anchalee Techasen (1) (2)
  Raynoo Thanan (1) (2)
  Kunyarat Duenngai (2) (3)
  Paiboon Sithithaworn (2) (4)
  Eimorn Mairiang (5)
  Watcharin Loilome (1) (2)
  Nisana Namwat (1) (2)
  Chawalit Pairojkul (2) (6)
  Puangrat Yongvanit (1) (2)
  
• 关键词：Opisthorchis viverrini ; Oxidized alpha ; 1 antitrypsin ; Risk marker ; Cholangiocarcinoma ; Periductal fibrosis
• 刊名：Tumor Biology
• 出版年：2013
• 出版时间：April 2013
• 年：2013
• 卷：34
• 期：2
• 页码：695-704
• 全文大小：547KB
• 参考文献：1. Sriplung H, Wiangnon S, Sontipong S, Sumitsawan Y, Martin N. Cancer incidence trends in Thailand, 1989-000. Asian Pac J Cancer Prev. 2006;7(2):239-4.
  2. Parkin DM. The global health burden of infection-associated cancers in the year 2002. Int J Cancer. 2006;118(12):3030-4. CrossRef
  3. Sithithaworn P, Andrews RH, Nguyen VD, Wongsaroj T, Sinuon M, Odermatt P, et al. The current status of opisthorchiasis and clonorchiasis in the Mekong Basin. Parasitol Int. 2012;61(1):10-. CrossRef
  4. Yongvanit P, Pinlaor S, Bartsch H. Oxidative and nitrative DNA damage: key events in opisthorchiasis-induced carcinogenesis. Parasitol Int. 2012;61(1):130-. CrossRef
  5. Yongvanit P, Thanan R, Pinlaor S, Sithithaworn P, Loilome W, Namwat N, et al. Increased expression of TLR-2, COX-2, and SOD-2 genes in the peripheral blood leukocytes of opisthorchiasis patients induced by / Opisthorchis viverrini antigen. Parasitol Res. 2012;110(5):1969-7.
  6. Pinlaor S, Ma N, Hiraku Y, Yongvanit P, Semba R, Oikawa S, et al. Repeated infection with / Opisthorchis viverrini induces accumulation of 8-nitroguanine and 8-oxo-7,8-dihydro-2-deoxyguanine in the bile duct of hamsters via inducible nitric oxide synthase. Carcinogenesis. 2004;25(8):1535-2. CrossRef
  7. Pinlaor S, Sripa B, Sithithaworn P, Yongvanit P. Hepatobiliary changes, antibody response, and alteration of liver enzymes in hamsters re-infected with / Opisthorchis viverrini. Exp Parasitol. 2004;108(1-):32-. CrossRef
  8. Boonmars T, Srisawangwong T, Srirach P, Kaewsamut B, Pinlaor S, Sithithaworn P. Apoptosis-related gene expressions in hamsters re-infected with / Opisthorchis viverrini and re-treated with praziquantel. Parasitol Res. 2007;102(1):57-2. CrossRef
  9. Boonmars T, Srirach P, Kaewsamut B, Srisawangwong T, Pinlaor S, Pinlaor P, et al. Apoptosis-related gene expression in hamster opisthorchiasis post praziquantel treatment. Parasitol Res. 2008;102(3):447-5. CrossRef
  10. Prakobwong S, Pinlaor S, Yongvanit P, Sithithaworn P, Pairojkul C, Hiraku Y. Time profiles of the expression of metalloproteinases, tissue inhibitors of metalloproteases, cytokines and collagens in hamsters infected with / Opisthorchis viverrini with special reference to peribiliary fibrosis and liver injury. Int J Parasitol. 2009;39(7):825-5. CrossRef
  11. Mairiang E, Elkins DB, Mairiang P, Chaiyakum J, Chamadol N, Loapaiboon V, et al. Relationship between intensity of / Opisthorchis viverrini infection and hepatobiliary disease detected by ultrasonography. J Gastroenterol Hepatol. 1992;7(1):17-1. CrossRef
  12. Mairiang E, Laha T, Bethony JM, Thinkhamrop B, Kaewkes S, Sithithaworn P, et al. Ultrasonography assessment of hepatobiliary abnormalities in 3359 subjects with / Opisthorchis viverrini infection in endemic areas of Thailand. Parasitol Int. 2012;61(1):208-1. CrossRef
  13. Thanan R, Oikawa S, Yongvanit P, Hiraku Y, Ma N, Pinlaor S. Inflammation-induced protein carbonylation contributes to poor prognosis of cholangiocarcinoma. Free Rad Biol Med. 2012.
  14. Taggart C, Cervantes-Laurean D, Kim G, McElvaney NG, Wehr N, Moss J, et al. Oxidation of either methionine 351 or methionine 358 in alpha 1-antitrypsin causes loss of anti-neutrophil elastase activity. J Biol Chem. 2000;275(35):27258-5.
  15. Banfi C, Brioschi M, Barcella S, Veglia F, Biglioli P, Tremoli E, et al. Oxidized proteins in plasma of patients with heart failure: role in endothelial damage. Eur J Heart Fail. 2008;10(3):244-1. CrossRef
  16. Sripa B, Mairiang E, Thinkhamrop B, Laha T, Kaewkes S, Sithithaworn P, et al. Advanced periductal fibrosis from infection with the carcinogenic human liver fluke / Opisthorchis viverrini correlates with elevated levels of interleukin-6. Hepatology. 2009;50(4):1273-1. CrossRef
  17. Boonjaraspinyo S, Boonmars T, Wu Z, Loilome W, Sithithaworn P, Nagano I, et al. Platelet-derived growth factor may be a potential diagnostic and prognostic marker for cholangiocarcinoma. Tumour Biol. 2012;33(5):1785-02.
  18. Loilome W, Yooyuen S, Namwat N, Sithithaworn P, Puapairoj A, Kano J, et al. PRKAR1A overexpression is associated with increased ECPKA autoantibody in liver fluke-associated cholangiocarcinoma: application for assessment of the risk group. Tumour Biol. 2012;33(6):2289-8.
  19. Van Beers BE. Diagnosis of cholangiocarcinoma. HPB (Oxford). 2008;10(2):87-3. CrossRef
  20. Chidwick K, Winyard PG, Zhang Z, Farrell AJ, Blake DR. Inactivation of the elastase inhibitory activity of alpha 1 antitrypsin in fresh samples of synovial fluid from patients with rheumatoid arthritis. Ann Rheum Dis. 1991;50(12):915-. CrossRef
  21. Sepper R, Konttinen YT, Ingman T, Sorsa T. Presence, activities, and molecular forms of cathepsin G, elastase, alpha 1-antitrypsin, and alpha 1-antichymotrypsin in bronchiectasis. J Clin Immunol. 1995;15(1):27-4. CrossRef
  22. Ueda M, Mashiba S, Uchida K. Evaluation of oxidized alpha-1-antitrypsin in blood as an oxidative stress marker using anti-oxidative alpha1-AT monoclonal antibody. Clin Chim Acta. 2002;317(1-):125-1. CrossRef
  23. Izumi-Yoneda N, Toda A, Okabe M, Koike C, Takashima S, Yoshida T, et al. Alpha 1 antitrypsin activity is decreased in human amnion in premature rupture of the fetal membranes. Mol Hum Reprod. 2009;15(1):49-7. CrossRef
  24. Mannello F, Gazzanelli G. Tissue inhibitors of metalloproteinases and programmed cell death: conundrums, controversies and potential implications. Apoptosis. 2001;6(6):479-2. CrossRef
  25. Remacle A, McCarthy K, Noel A, Maguire T, McDermott E, O'Higgins N, et al. High levels of TIMP-2 correlate with adverse prognosis in breast cancer. Int J Cancer. 2000;89(2):118-1. CrossRef
  26. Shamamian P, Schwartz JD, Pocock BJ, Monea S, Whiting D, Marcus SG, et al. Activation of progelatinase A (MMP-2) by neutrophil elastase, cathepsin G, and proteinase-3: a role for inflammatory cells in tumor invasion and angiogenesis. J Cell Physiol. 2001;189(2):197-06. CrossRef
  27. Leelawat K, Sakchinabut S, Narong S, Wannaprasert J. Detection of serum MMP-7 and MMP-9 in cholangiocarcinoma patients: evaluation of diagnostic accuracy. BMC Gastroenterol. 2009;9:30. CrossRef
  
• 作者单位：Wassana Jamnongkan (1) (2)
  Anchalee Techasen (1) (2)
  Raynoo Thanan (1) (2)
  Kunyarat Duenngai (2) (3)
  Paiboon Sithithaworn (2) (4)
  Eimorn Mairiang (5)
  Watcharin Loilome (1) (2)
  Nisana Namwat (1) (2)
  Chawalit Pairojkul (2) (6)
  Puangrat Yongvanit (1) (2)
  
  1. Department of Biochemistry, Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand
  2. Liver Fluke and Cholangiocarcinoma Research Center, Khon Kaen University, Khon Kaen, 40002, Thailand
  3. Department of Biomedical Science Program, Graduate School, Khon Kaen University, Khon Kaen, 40002, Thailand
  4. Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand
  5. Department of Radiology, Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand
  6. Department of Pathology, Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand
  
• ISSN：1423-0380

文摘

The oxidized alpha-1 antitrypsin (ox-A1AT) is one modified form of A1AT, generated via oxidation at its active site by free radicals released from inflammatory cells which subsequently are unable to inhibit protease enzymes. The presence of ox-A1AT in human serum has been used as oxidative stress indicator in many diseases. As oxidative/nitrative damage is one major contributor in opisthorchiasis-driven cholangiocarcinogenesis, we determined A1AT and ox-A1AT expression in human cholangiocarcinoma (CCA) tissue using immunohistochemical staining and measured serum ox-A1AT levels by ELISA. A1AT and ox-A1AT were found to be expressed in the tumor of CCA patients. The group with high expression has a significant poor prognosis. Serum levels of ox-A1AT were also significantly higher in groups of patients with heavy Opisthorchis viverrini infection, advanced periductal fibrosis (APF) and CCA when compared with healthy controls (P-lt;-.001). Odds ratio (OR) analysis implicated high ox-A1AT levels as a risk predictor for APF and CCA (P-lt;-.001; OR--40.5 and 22.0, respectively). In conclusion, as APF may lead to hepatobiliary diseases and an increased risk of CCA development, our results identified ox-A1AT as a potential risk indicator for opisthorchiasis-associated CCA. This marker could now be explored for screening of subjects living in endemic areas where the prevalence of opisthorchiasis still remains high.