Valorization of Palm Oil Industrial Waste as Feedstock for Lipase Production

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• 作者：Erick A. Silveira ; Paulo W. Tardioli…
• 刊名：Applied Biochemistry and Biotechnology
• 出版年：2016
• 出版时间：June 2016
• 年：2016
• 卷：179
• 期：4
• 页码：558-571
• 全文大小：887 KB
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Biotechnology
  Biochemistry
  
• 出版者：Humana Press Inc.
• ISSN：1559-0291
• 卷排序：179

文摘

The use of residues from the industrial processing of palm oil as carbon source and inducer for microbial lipase production can be a way to add value to such residues and to contribute to reduced enzyme costs. The aim of this work was to investigate the feasibility of using palm oil industrial waste as feedstock for lipase production in different cultivation systems. Evaluation was made of lipase production by a selected strain of Aspergillus niger cultivated under solid-state (SSF) and submerged fermentation (SmF). Lipase activity levels up to 15.41 IU/mL were achieved under SSF. The effects of pH and temperature on the lipase activity of the SSF extract were evaluated using statistical design methodology, and maximum activities were obtained between pH 4.0 and 6.5 and at temperatures between 37 and 55 °C. This lipase presented good thermal stability up to 60 °C and higher specificity towards long carbon chain substrates. The results demonstrate the potential application of palm oil industrial residues for lipase production and contribute to the technological advances needed to develop processes for industrial enzymes production.KeywordsLipaseAgro-industrial wastesPalm oil residuesBiorefinerySubmerged fermentationSolid-state fermentation