Taxonomic status and origin of the Egyptian weasel (Mustela subpalmata) inferred from mitochondrial DNA

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• 作者：Mónica Rodrigues ; Arthur R. Bos ; Richard Hoath ; Patrick J. Schembri…
• 关键词：Mustela subpalmata ; Species status ; Molecular systematics ; Phylogeography ; Mitochondrial DNA ; Weasels
• 刊名：Genetica
• 出版年：2016
• 出版时间：April 2016
• 年：2016
• 卷：144
• 期：2
• 页码：191-202
• 全文大小：1,576 KB
• 参考文献：Abramov AV, Baryshnikov GF (2000) Geographic variation and intraspecific taxonomy of weasel Mustela nivalis (Carnivora, Mustelidae). Zoosyste Ross 8:365–402
  Alatalo RV, Gustafsson L (1988) Genetic component of morphological differentiation in coal tits under competitive release. Evolution 42:200–203CrossRef
  Angerbjörn A (1986) Gigantism in island populations of wood mice (Apodemus) in Europe. Oikos 47:47–56CrossRef
  Avise JC, Ball RM (1990) Principles of genealogical concordance in species concepts and biological taxonomy. Oxf Surv Evol Biol 7:45–67
  Baker PJ, Harris S (2007) Urban mammals: what does the future hold? An analysis of the factors affecting patterns of use of residential gardens in Great Britain. Mamm Rev 37:297–315
  Ballard RD, Stager LE, Master D, Yoerger D, Mindell D, Whitcomb LL, Singh H, Piechota D (2002) Iron age shipwrecks in deep water off Ashkelon, Israel. Am J Archaeol 106:151–168CrossRef
  Bandelt H, Forster P, Röhl A (1999) Median-joining networks for inferring intraspecific phylogenies. Mol Biol Evol 16:37–48PubMed CrossRef
  Bar-Oz G, Dayan T (2001) Weasels from the Hellenistic period of Israel. Isr J Zool 47:271–274CrossRef
  Barrett-Hamilton GEH (1900) Note on the weasel, Putorius (Ictis) nivalis, Linn., and some of its subspecies. J Nat Hist 5:41–50CrossRef
  Bate DMA (1905) On the mammals of Crete. Proc Zool Soc Lond 75:315–323
  Bateman PW, Fleming PA (2012) Big city life: carnivores in urban environments. J Zool 287:1–23CrossRef
  Berry V, Gascuel O (1996) Interpretation of bootstrap trees: threshold of clade selection and induced gain. Mol Biol Evol 13:999–1011CrossRef
  Bradley RD, Baker RJ (2001) A test of the genetic species concept: cytochrome-b sequences and mammals. J Mammal 82:960–973CrossRef
  Costa M, Fernandes C, Birk JDS, Kitchener AC, Santos-Reis M, Bruford W (2013) The genetic legacy of the 19th-century decline of the British polecat: evidence for extensive introgression from feral ferrets. Mol Ecol 22:5130–5147PubMed CrossRef
  Cretzschmar PJ (1826) Atlas zu der Reise im nördlichen Afrika von Eduard Rüppell. Erste Abteilung Zoologie. Senckenbergischen naturforschenden Gesellschaft, Frankfurt am Main
  Cucchi T, Vigne JD (2006) Origin and diffusion of the house mouse in the Mediterranean. Hum Evol 21:95–106CrossRef
  Darriba D, Taboada GL, Doallo R, Posada D (2012) jModelTest 2: more models, new heuristics and parallel computing. Nat Methods 9:772PubMed PubMedCentral CrossRef
  Dayan T (1997) Weasels from the Iron Age of Israel: a biogeographic note. Isr J Zool 43:295–298
  Dayan T, Tchernov E (1988) On the first occurrence of the common weasel (Mustela nivalis) in the fossil record of Israel. Mammalia 52:165–168CrossRef
  Dobson M (1998) Mammal distributions in the western Mediterranean: the role of human intervention. Mamm Rev 28:77–88CrossRef
  Driscoll CA, Menotti-Raymond M, Roca AL, Hupe K, Johnson WE, Geffen E, Harley EH, Delibes M, Dominique P, Kitchner AC, Yamaguchi N, O’Brien J, Macdonald DW (2007) The Near Eastern origin of cat domestication. Science 317:519–523PubMed CrossRef
  East K, Lockie JD (1964) Observations on a family of weasels (Mustela nivalis) bred in captivity. Proc Zool Soc Lond 143:359–363
  Erlinge S (1979) Adaptive significance of sexual dimorphism in weasels. Oikos 33:233–245CrossRef
  Erlinge S (1987) Why do European stoats Mustela erminea not follow Bergmann’s rule? Ecography 10:33–39CrossRef
  Evian SBD (2011) Egypt and the levant in the Iron Age I–IIA: the ceramic evidence. Tel Aviv 38:94–119CrossRef
  Excoffier L, Lischer HE (2010) Arlequin suite ver 3.5: a new series of programs to perform population genetics analyses under Linux and Windows. Mol Ecol Resour 10:564–567PubMed CrossRef
  Excoffier L, Smouse PE, Quattro JM (1992) Analysis of molecular variance inferred from metric distances among DNA haplotypes: application to human mitochondrial DNA restriction data. Genetics 131:479–491PubMed PubMedCentral
  Fernandes CA, Ginja C, Pereira I, Tenreiro R, Bruford MW, Santos-Reis M (2008) Species-specific mitochondrial DNA markers for identification of non-invasive samples from sympatric carnivores in the Iberian Peninsula. Conserv Genet 9:681–690CrossRef
  Finné M, Holmgren K, Sundqvist HS, Weiberg E, Lindblom M (2011) Climate in the eastern Mediterranean, and adjacent regions, during the past 6000 years—a review. J Archaeol Sci 38:3153–3173CrossRef
  Flower SS (1932) 22. Notes on the recent mammals of Egypt, with a list of the species recorded from that kingdom. Proc Zool Soc Lond 102:369–450CrossRef
  Frank F (1985) Zur Evolution und Systematik der kleinen Wiesel (Mustela nivalis Linnaeus, 1766). Z Saugetierkd 50:208–225
  Fraser DJ, Bernatchez L (2001) Adaptive evolutionary conservation: towards a unified concept for defining conservation units. Mol Ecol 10:2741–2752PubMed CrossRef
  Frynta D, Slabova M, Vachova H, Volfova R, Munclinger P (2005) Aggression and commensalism in house mouse: a comparative study across Europe and the near east. Aggress Behav 31:283–293CrossRef
  Fu YX (1997) Statistical tests of neutrality of mutations against population growth, hitchhiking and background selection. Genetics 147:915–925PubMed PubMedCentral
  Garcia-Rodriguez AI, Bowen BW, Domning D, Mignucci-Giannoni AA, Marmontel M, Montoya-Ospina RA, Morales-Vela B, Rudin M, Bonde RK, McGuire PM (1998) Phylogeography of the West Indian manatee (Trichechus manatus): how many populations and how many taxa? Mol Ecol 7:1137–1149PubMed CrossRef
  Gippoliti S, Amori G (2002) Anthropochorous wild mammal taxa and conservation lists. Conserv Biol 6:1162–1164CrossRef
  Golubchik T, Wise MJ, Easteal S, Jermiin LS (2007) Mind the gaps: evidence of bias in estimates of multiple sequence alignments. Mol Biol Evol 24:2433–2442PubMed CrossRef
  Goudsmit J, Brandon-Jones D (2000) Evidence from the baboon catacomb in North Saqqara for a west Mediterranean monkey trade route to Ptolemaic Alexandria. J Egypt Archaeol 86:111–119CrossRef
  Grant PR (1972) Convergent and divergent character displacement. Biol J Linn Soc 4:39–68CrossRef
  Handwerk VJ (1993) Zur Biologie und Ökologie ägyptischer Wiesel, Mustela subpalmata Hemprich und Ehrenberg, 1833. Zool Middle East 9:5–30
  Hanke M, Wink M (1994) Direct DNA sequencing of PCR-amplified vector inserts following enzymatic degradation of primer and dNTPs. Biotechniques 17:858–860PubMed
  Harding LE, Smith FA (2009) Mustela or Vison? Evidence for the taxonomic status of the American mink and a distinct biogeographic radiation of American weasels. Mol Phylogenet Evol 52:632–642PubMed CrossRef
  Hoath R (2009) A field guide to the mammals of Egypt. The American University in Cairo Press, Cairo
  Holder MT, Sukumaran J, Lewis PO (2008) A justification for reporting the majority-rule consensus tree in Bayesian phylogenetics. Syst Biol 57:814–821PubMed CrossRef
  Hu Y, Hu S, Wang W, Wu X, Marshall FB, Chen X, Hou L, Wang C (2014) Earliest evidence for commensal processes of cat domestication. Proc Natl Acad Sci 111:116–120PubMed PubMedCentral CrossRef
  Huelsenbeck JP, Larget B, Alfaro ME (2004) Bayesian phylogenetic model selection using reversible jump Markov chain Monte Carlo. Mol Biol Evol 21:1123–1133PubMed CrossRef
  Kaniewski D, Paulissen E, Van Campo E, Al-Maqdissi M, Bretschneider J, Van Lerberghe K (2008) Middle East coastal ecosystem response to middle-to-late Holocene abrupt climate changes. Proc Natl Acad Sci 105:13941–13946PubMed PubMedCentral CrossRef
  King CM (1989) The advantages and disadvantages of small size to weasels, Mustela species. In: Gittleman JL (ed) Carnivore behavior, ecology, and evolution. Chapman and Hall, London, pp 302–334CrossRef
  Kingdon J (2001) The Kingdon field guide to African mammals. Academic Press, London
  Koepfli KP, Deere KA, Slater GJ, Begg C, Begg K, Grassman L, Lucherini M, Veron G, Wayne RK (2008) Multigene phylogeny of the Mustelidae: resolving relationships, tempo and biogeographic history of a mammalian adaptive radiation. BMC Biol 6:10PubMed PubMedCentral CrossRef
  Kowalski K, Rzebik-Kowalska B (1991) Mammals of algeria. Ossolineum, Wroclaw
  Kurose N, Abramov AV, Masuda R (2005) Comparative phylogeography between the ermine Mustela erminea and the least weasel M. nivalis of Palaearctic and Nearctic regions, based on analysis of mitochondrial DNA control region sequences. Zool Sci 22:1069–1078PubMed CrossRef
  Kurose N, Abramov AV, Masuda R (2008) Molecular phylogeny and taxonomy of the genus Mustela (Mustelidae, Carnivora), inferred from mitochondrial DNA sequences: new perspectives on phylogenetic status of the back-striped weasel and American mink. Mamm Study 33:25–33CrossRef
  Lande R (1980) Sexual dimorphism, sexual selection, and adaptation in polygenic characters. Evolution 34:292–305CrossRef
  Lebarbenchon C, Poitevin F, Montgelard C (2006) Genetic variation of the weasel (Mustela nivalis) in Corsica based on mitochondrial control region sequences. Mamm Biol 71:164–171
  Lebarbenchon C, Poitevin F, Arnal V, Montgelard C (2010) Phylogeography of the weasel (Mustela nivalis) in the western-Palaearctic region: combined effects of glacial events and human movements. Heredity 105:449–462PubMed CrossRef
  Lehmann EV, Nobis G (1979) Subfossile Mauswiesel, Mustela nivalis Linne, 1766 aus Enkomi-Alasia auf Zypern. Bonn Zool Beitr 30:32–38
  Lesica P, Allendorf FW (1995) When are peripheral populations valuable for conservation? Conserv Biol 9:753–760CrossRef
  Lomolino MV (2005) Body size evolution in insular vertebrates: generality of the island rule. J Biogeogr 32:1683–1699CrossRef
  Lomolino MV, Channell R (1995) Splendid isolation: patterns of geographic range collapse in endangered mammals. J Mamm 76:335–347CrossRef
  Luo A, Qiao H, Zhang Y, Shi W, Ho SY, Xu W, Zhang A, Zhu C (2010) Performance of criteria for selecting evolutionary models in phylogenetics: a comprehensive study based on simulated datasets. BMC Evol Biol 10:242PubMed PubMedCentral CrossRef
  Marciszak A, Socha P (2014) Stoat Mustela erminea Linnaeus, 1758 and weasel Mustela nivalis Linnaeus, 1766 in palaeoecological analysis: a case study of Biśnik Cave. Quat Int 339–340:258–265CrossRef
  Masseti M (1995) Quaternary biogeography of the Mustelidae family on the Mediterranean islands. Hystrix 7:17–34
  McDevitt AD, Zub K, Kawałko A, Oliver MK, Herman JS, Wójcik JM (2012) Climate and refugial origin influence the mitochondrial lineage distribution of weasels (Mustela nivalis) in a phylogeographic suture zone. Biol J Linn Soc 106:57–69CrossRef
  McDonald R, Hoffmann M (2008) Mustela subpalmata. The IUCN red list of threatened species. doi:10.​2305/​IUCN.​UK.​2008.​RLTS.​T41660A10504103.​en
  Miller GS (1912) Catalogue of the mammals of Western Europe (Europe exclusive of Russia) in the collection of the British Museum. Trustees of the British Museum, London
  Moors PJ (1975) The food of weasels (Mustela nivalis) on farmland in north-east Scotland. J Zool 177:455–461CrossRef
  Moors PJ (1980) Sexual dimorphism in the body size of mustelids (Carnivora): the roles of food habits and breeding systems. Oikos 34:147–158CrossRef
  Morrison DA (2007) Increasing the efficiency of searches for the maximum likelihood tree in a phylogenetic analysis of up to 150 nucleotide sequences. Syst Biol 56:988–1010PubMed CrossRef
  Muse SV, Gaut BS (1994) A likelihood approach for comparing synonymous and nonsynonymous nucleotide substitution rates, with application to the chloroplast genome. Mol Biol Evol 11:715–724PubMed
  Negbi O (1992) Early Phoenician presence in the Mediterranean islands: a reappraisal. Am J Archaeol 96:599–615CrossRef
  Nei M (1987) Molecular evolutionary genetics. Columbia University Press, New York
  Niethammer J (1973) Das Mauswiesel (Mustela nivalis) in Afghanistan. Bonn Zool Beitr 24:1–6
  Niewiarowski PH, Roosenburg W (1993) Reciprocal transplant reveals sources of variation in growth rates of the lizard Sceloporus undulatus. Ecology 74:1992–2002CrossRef
  Nye TM, Lio P, Gilks WR (2006) A novel algorithm and web-based tool for comparing two alternative phylogenetic trees. Bioinformatics 22:117–119PubMed CrossRef
  O’Brien SJ, Mayr E (1991) Bureaucratic mischief: recognizing endangered species and subspecies. Science 251:1187–1188PubMed CrossRef
  Osborn DJ, Helmy I (1980) The contemporary land mammals of Egypt (including Sinai). Fieldiana Zool 5:1–579
  Pesole G, Gissi C, De Chirico A, Saccone C (1999) Nucleotide substitution rate of mammalian mitochondrial genomes. J Mol Evol 48:427–434PubMed CrossRef
  Pond SLK, Frost SD (2005) Not so different after all: a comparison of methods for detecting amino acid sites under selection. Mol Biol Evol 22:1208–1222CrossRef
  Pond SLK, Frost SDW, Muse SV (2005) HyPhy: hypothesis testing using phylogenies. Bioinformatics 21:676–679PubMed CrossRef
  Poulakakis N, Lymberakis P, Tsigenopoulos CS, Magoulas A, Mylonas M (2005) Phylogenetic relationships and evolutionary history of snake-eyed skink Ablepharus kitaibelii (Sauria: Scincidae). Mol Phylogenet Evol 34:245–256PubMed CrossRef
  Ralls K, Harvey PH (1985) Geographic variation in size and sexual dimorphism of North American weasels. Biol J Linn Soc 25:119–167CrossRef
  Reig S (1997) Biogeographic and evolutionary implications of size variation in North American least weasels (Mustela nivalis). Can J Zool 75:2036–2049CrossRef
  Romer AS (1928) Pleistocene mammals of Algeria: fauna of the Paleolithic station of Mechta-el-Arbi. Logan Mus Bull 1:80–163
  Ronquist F, Teslenko M, van der Mark P, Ayres DL, Darling A, Höhna S, Larget B, Liu L, Suchard MA, Huelsenbeck JP (2012) Mrbayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Syst Biol 61:539–542PubMed PubMedCentral CrossRef
  Ruffino L, Vidal E (2010) Early colonization of Mediterranean islands by Rattus rattus: a review of zooarcheological data. Biol Invasions 12:2389–2394CrossRef
  Sato JJ, Wolsan M, Prevosti FJ, D’Elía G, Begg C, Begg K, Hosoda T, Campbell KL, Suzuki H (2012) Evolutionary and biogeographic history of weasel-like carnivorans (Musteloidea). Mol Phylogenet Evol 63:745–757PubMed CrossRef
  Savage DE, Russell DE (1983) Mammalian paleofaunas of the world. Addison-Wesley, Reading
  Schwarz G (1978) Estimating the dimension of a model. Ann Stat 6:461–464CrossRef
  Setzer HW (1952) Notes on mammals from the Nile Delta region of Egypt. Proc US Natl Mus 102:343–369CrossRef
  Sheffield SR, King CM (1994) Mustela nivalis. Mamm Species 454:1–10CrossRef
  Staubwasser M, Weiss H (2006) Holocene climate and cultural evolution in late prehistoric–early historic West Asia. Quat Res 66:372–387CrossRef
  Stearns SC (1989) The evolutionary significance of phenotypic plasticity. Bioscience 39:436–445CrossRef
  Stöver BC, Müller KF (2010) TreeGraph 2: combining and visualizing evidence from different phylogenetic analyses. BMC Bioinform 11:7CrossRef
  Sukumaran J, Holder MT (2010) DendroPy: a Python library for phylogenetic computing. Bioinformatics 26:1569–1571PubMed CrossRef
  Tajima F (1989) Statistical method for testing the neutral mutation hypothesis by DNA polymorphism. Genetics 123:585–595PubMed PubMedCentral
  Tamar K, Carranza S, Sindaco R, Moravec J, Meiri S (2014) Systematics and phylogeography of Acanthodactylus schreiberi and its relationships with Acanthodactylus boskianus (Reptilia: Squamata: Lacertidae). Zool J Linn Soc 172:720–739CrossRef
  Tamura K, Nei M (1993) Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Mol Biol Evol 10:512–526PubMed
  Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30:2725–2729PubMed PubMedCentral CrossRef
  Thalmann O, Shapiro B, Cui P, Schuenemann VJ, Sawyer SK, Greenfield DL, Germonpré MB, Sablin MV, López-Giráldez F, Domingo-Roura X, Napierala H, Uerpmann H-P, Loponte DM, Acosta AA, Giemsch L, Schmitz RW, Worthington B, Buikstra JE, Druzhkova A, Graphodatsky AS, Ovodov ND, Wahlberg N, Freedman AH, Schweizer RM, Koepfli KP, Leonard JA, Meyer M, Krause J, Pääbo S, Green RE, Wayne RK (2013) Complete mitochondrial genomes of ancient canids suggest a European origin of domestic dogs. Science 342:871–874PubMed CrossRef
  Thomas O (1895) On the long-lost Putorius africanus, Desm., and its occurrence in Malta. Proc Zool Soci Lond 63:128–131
  Thompson JD, Linard B, Lecompte O, Poch O (2011) A comprehensive benchmark study of multiple sequence alignment methods: current challenges and future perspectives. PLoS One 6:e18093PubMed PubMedCentral CrossRef
  Tikhonov A, Cavallini P, Maran T, Kranz A, Herrero J, Giannatos G, Stubbe M, Conroy J, Kryštufek B, Abramov A, Wozencraft C, Reid F, McDonald R (2008) Mustela nivalis. The IUCN red list of threatened species. doi:10.​2305/​IUCN.​UK.​2008.​RLTS.​T14021A4383128.​en
  van Zyll de Jong CG (1992) A morphometric analysis of cranial variation in Holarctic weasels (Mustela nivalis). Z Saugetierkd 57:77–93
  Villesen P (2007) FaBox: an online toolbox for fasta sequences. Mol Ecol Notes 7:965–968CrossRef
  Wallace IM, O’Sullivan O, Higgins DG, Notredame C (2006) M-Coffee: combining multiple sequence alignment methods with T-Coffee. Nucleic Acid Res 34:1692–1699PubMed PubMedCentral CrossRef
  Weinstein JM (1981) The Egyptian empire in Palestine: a reassessment. Bull Am Schools Orient Res 241:1–28CrossRef
  West-Eberhard MJ (1989) Phenotypic plasticity and the origins of diversity. Annu Rev Ecol Syst 20:249–278CrossRef
  Whittaker DJ, Ting N, Melnick DJ (2006) Molecular phylogenetic affinities of the simakobu monkey (Simias concolor). Mol Phylogenet Evol 39:887–892PubMed CrossRef
  Xia X (2013) DAMBE5: a comprehensive software package for data analysis in molecular biology and evolution. Mol Biol Evol 30:1720–1728PubMed PubMedCentral CrossRef
  Xia X, Xie Z, Salemi M, Chen L, Wang Y (2003) An index of substitution saturation and its application. Mol Phylogenet Evol 26:1–7PubMed CrossRef
  Yom-Tov Y (2003) Body sizes of carnivores commensal with humans have increased over the past 50 years. Funct Ecol 17:323–327CrossRef
  Zwickl DJ (2006) Genetic algorithm approaches for the phylogenetic analysis of large biological sequence datasets under the maximum likelihood criterion. Ph.D. dissertation, The University of Texas at Austin
  
• 作者单位：Mónica Rodrigues (1) (2)
  Arthur R. Bos (3) (4)
  Richard Hoath (3)
  Patrick J. Schembri (5)
  Petros Lymberakis (6)
  Michele Cento (7)
  Wissem Ghawar (8)
  Sakir O. Ozkurt (9)
  Margarida Santos-Reis (1)
  Juha Merilä (2)
  Carlos Fernandes (1)
  
  1. CE3C – Centre for Ecology, Evolution and Environmental Changes, Faculdade de Ciências, Universidade de Lisboa, 1749-016, Lisbon, Portugal
  2. Ecological Genetics Research Unit, Department of Biosciences, University of Helsinki, PO Box 65, 00014, Helsinki, Finland
  3. The American University in Cairo, PO Box 74, New Cairo, 11835, Egypt
  4. Naturalis Biodiversity Centre, PO Box 9517, 2300 RA, Leiden, The Netherlands
  5. Department of Biology, University of Malta, Msida, MSD 2080, Malta
  6. Natural History Museum of Crete, University of Crete, 71409, Iráklion, Crete, Greece
  7. SROPU Bird Observation and Protection Station of Rome, 00165, Rome, Italy
  8. Service of Medical Epidemiology, Institut Pasteur de Tunis, Tunis-Belvédère, Tunisia
  9. Faculty of Education, Ahi Evran University, Kırşehir, Turkey
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Life Sciences
  Animal Genetics and Genomics
  Plant Genetics and Genomics
  Human Genetics
  Microbial Genetics and Genomics
  
• 出版者：Springer Netherlands
• ISSN：1573-6857

文摘

The Egyptian weasel (Mustela subpalmata) is a small mustelid with a distribution restricted to the lower Nile Valley and the Nile Delta. Traditionally considered a subspecies of the least weasel (M. nivalis), it is currently recognized as a separate species based on morphology. Here we present the first genetic assessment of the taxonomic status of the Egyptian weasel by comparing mitochondrial DNA (Cytochrome b gene and control region) sequences to those of least weasels from the western Palearctic, with a focus on the Mediterranean region. Our results provide no evidence to support the view that the Egyptian weasel is genetically distinct from the least weasel, as we found that, for both Cytochrome b and control region, haplotypes were shared between the two taxa. Specifically, the Cytochrome b and control region haplotypes detected in the Egyptian weasel were also present in M. nivalis from Turkey and Malta, two populations genetically analysed here for the first time. Our results suggest that the Egyptian weasel is distinct from the least weasel populations currently living in the Maghreb, which were inferred to be the result of an earlier colonization of North Africa, but the genetic data alone do not allow us to determine whether the Egyptian weasel is native or introduced. Nevertheless, the observed genetic patterns, together with the weasel fossil record in Israel and the unique commensal lifestyle of the Egyptian weasel, are consistent with the hypothesis that the Egyptian population is a relict of past range expansion from the Levant into Egypt. We suggest that the large size and characteristic sexual dimorphism of the Egyptian weasel are likely to represent ecotypic variation, but genomic studies are required to clarify the extent of its functional genetic divergence.